Journal
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 63, Issue 11, Pages 1868-1887Publisher
WILEY
DOI: 10.1111/jipb.13161
Keywords
Arabidopsis; CBF target genes; ChIP-seq; cold acclimation
Categories
Funding
- National Natural Sciences Foundation of China [31872658, 32022008, 31921001]
Ask authors/readers for more resources
Cold acclimation in Arabidopsis thaliana triggers significant transcriptional reprogramming through the CBF-dependent pathway, involving CBF transcription factors binding to conserved sequences on target genes. CBF-target genes are enriched in functions related to hormones, light, and circadian rhythm signaling, indicating their role as key integrators of endogenous and external cues.
Cold acclimation in Arabidopsis thaliana triggers a significant transcriptional reprogramming altering the expression patterns of thousands of cold-responsive (COR) genes. Essential to this process is the C-repeat binding factor (CBF)-dependent pathway, involving the activity of AP2/ERF (APETALA2/ethylene-responsive factor)-type CBF transcription factors required for plant cold acclimation. In this study, we performed chromatin immunoprecipitation assays followed by deep sequencing (ChIP-seq) to determine the genome-wide binding sites of the CBF transcription factors. Cold-induced CBF proteins specifically bind to the conserved C-repeat (CRT)/dehydration-responsive elements (CRT/DRE; G/ACCGAC) of their target genes. A Gene Ontology enrichment analysis showed that 1,012 genes are targeted by all three CBFs. Combined with a transcriptional analysis of the cbf1,2,3 triple mutant, we define 146 CBF regulons as direct CBF targets. In addition, the CBF-target genes are significantly enriched in functions associated with hormone, light, and circadian rhythm signaling, suggesting that the CBFs act as key integrators of endogenous and external environmental cues. Our findings not only define the genome-wide binding patterns of the CBFs during the early cold response, but also provide insights into the role of the CBFs in regulating multiple biological processes of plants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available