☆ 4.7 Article

The elucidation of the biodegradation of nitrobenzene and p-nitrophenol of nitroreductase from Antarctic psychrophile Psychrobacter sp. ANT206 under low temperature

JOURNAL OF HAZARDOUS MATERIALS (2021)

Journal

JOURNAL OF HAZARDOUS MATERIALS

Volume 413, Issue -, Pages -

Publisher

ELSEVIER

DOI: 10.1016/j.jhazmat.2021.125377

Keywords

Nitroreductase; Deletion mutant; Psychrobacter sp.; Nitro-aromatic compounds; Metabolomics

Funding

National Natural Science Foundation of China [41876149]
Key Research and Development Program of Shandong Province, China [2019GHY112031]
Natural Science Foundation of Shandong Province, China [ZR2019MD018]

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Abstract

In this study, a deletion mutant strain with well genetic stability of the psntr gene was constructed using the allelic replacement method. The psntr gene was found to be involved in the regulation of tolerance against nitro-aromatic compounds in Psychrobacter from Antarctica. Metabolite analysis by GC-MS identified differences between the wildtype strain and the psntr mutant strain under nitrobenzene and p-nitrophenol stress.

Psychrobacter is one important typical strain in the Antarctic environment. In our previous study, Psychrobacter sp. ANT206 from Antarctica with novel cold-adapted nitroreductase (PsNTR) could biodegrade nitrobenzene and p-nitrophenol in low temperature environment. In this study, the in-frame deletion mutant of psntr (Delta psntr-ANT206) that displayed well genetic stability and kanamycin resistance stability was constructed using allelic replacement method. Additionally, Delta psntr-ANT206 was more sensitive to nitrobenzene and p-nitrophenol in the comparison of heat and hyperosmolarity, suggesting that psntr gene participated in the regulation of the tolerance against nitro-aromatic compounds (NACs). Further analysis was conducted by integrated gas chromatography-mass spectrometry (GC-MS), and several metabolites were identified. Among them, ethylbenzene, L-Alanine, citric acid, aniline, 4-aminophenol and other metabolites were different between the wildtype strain and.psntr-ANT206 under nitrobenzene and p-nitrophenol stress at different time periods under low temperature, respectively. These data could increase the knowledge of the construction of deletion mutant strains and biodegradation mechanism of NACs of typical strains Psychrobacter from Antarctica, which would also provide the basis of the molecular technique on the regulation of bioremediation of the contaminants under low temperature in the future.

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Immobilization of psychrophile Psychrobacter sp. ANT206 onto novel reusable magnetic nanoparticles and its application for nitro-aromatic compounds biodegradation under low temperature

Yifan Wang, Yanhua Hou, Yatong Wang, Ailin Zhang, Quanfu Wang

Summary: In this study, dopamine-functionalized magnetic nanoparticles were synthesized and used to immobilize nitroreductase-producing Psychrobacter sp. ANT206 for the efficient degradation of nitro-aromatic compounds at low temperature. The immobilized cells displayed excellent salinity stability and reusability, making them ideal for large-scale bioremediation of hazardous NACs.

BIODEGRADATION (2022)