Article
Agronomy
Tianfei Peng, Xuemei Liu, Fayi Tian, Hongfei Xu, Fengting Yang, Xuewei Chen, Xiwu Gao, Yuntong Lv, Jianyi Li, Yiou Pan, Qingli Shang
Summary: The study revealed that the upregulation of certain cytochrome P450 genes is associated with spirotetramat resistance in A. gossypii, and some lncRNAs may regulate the expression of these P450 genes to affect resistance. These findings provide a molecular basis for further investigating cotton aphid P450 genes and lncRNA functions in spirotetramat resistance development.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Jing Yang, Buli Fu, Peipan Gong, Chengjia Zhang, Xuegao Wei, Cheng Yin, Mingjiao Huang, Chao He, Tianhua Du, Jinjin Liang, Shaonan Liu, Yao Ji, Hu Xue, Chao Wang, Jinyu Hu, He Du, Rong Zhang, Xin Yang, Youjun Zhang
Summary: In this study, we investigated the role of CYP6CX2 and CYP6CX3 in mediating whitefly thiamethoxam resistance. Our results showed that the mRNA levels of these two genes were up-regulated after exposure to thiamethoxam. Transcriptional levels were overexpressed in resistant strains and positively correlated with resistance levels. Silencing these genes increased whitefly susceptibility to thiamethoxam. These findings provide insights into the role of cytochrome P450 monooxygenases in neonicotinoid resistance and suggest their potential as target genes for pest management.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Melanie Nolden, Mark J. I. Paine, Ralf Nauen
Summary: Pyrethroid resistance in Anopheles funestus is a major threat to the eradication of malaria. This study identified the enzymes CYP6P9a and CYP6P9b as major drivers of pyrethroid resistance in An. funestus, and investigated their interactions with azole-fungicides and pyrethroids. The study found that the mutation V310I in CYP6P9b increased its metabolic activity towards pyrethroids, particularly permethrin. Additionally, the study discovered the first metabolites of permethrin and deltamethrin, which were also suitable substrates for the CYP6P9 variants and were depleted to a higher extent than their parent compounds. Furthermore, it was confirmed that CYP6P9a and CYP6P9b are capable of metabolizing pyrethroids. These findings provide new insights into the molecular mechanisms of pyrethroid resistance in An. funestus.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tianxiang Xiao, Kai Lu
Summary: The common cutworm, Spodoptera litura, has developed resistance to commonly used insecticides. Two P450 enzymes, CYP6AE43 and CYP6AE48, play a crucial role in detoxifying pyrethroids and promoting resistance development in S. litura.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Shuai Wu, Minrong He, Fujin Xia, Xueyi Zhao, Xun Liao, Rongyu Li, Ming Li
Summary: This study found that the development of acetamiprid resistance in Nilaparvata lugens may be related to the overexpression of the P450 gene CYP6ER1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Farman Ullah, Hina Gul, Kaleem Tariq, Muhammad Hafeez, Nicolas Desneux, Dunlun Song
Summary: This study uncovered the role of the CYP6CY14 and CYP6DC1 genes in the development of clothianidin resistance in the cotton-melon aphid. Silencing these genes increased the sensitivity of the aphids to clothianidin, indicating their involvement in detoxification mechanisms. These findings emphasize the importance of these genes in insecticide resistance development.
ENTOMOLOGIA GENERALIS
(2023)
Article
Agronomy
Pei-Rong Li, Yu Shi, Di Ju, Yu-Xi Liu, Wei Wang, Ying-Shi He, Yu-Yun Zhang, Xue-Qing Yang
Summary: The study found that functional redundancy and preference of metabolism by CYP9A genes in the CYP9A subfamily contribute to lambda-cyhalothrin resistance in Cydia pomonella. These genes are highly expressed in the larval midgut and are induced by lambda-cyhalothrin. Knockdown experiments and in vitro assays confirmed the metabolic roles of these genes in lambda-cyhalothrin. These findings reveal the molecular mechanism of P450-mediated lambda-cyhalothrin resistance and its adaptive evolutionary strategy in Cydia pomonella.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Melanie Nolden, Mark J. Paine, Ralf Nauen
Summary: Cytochrome P450 monooxygenases play a crucial role in the oxidative metabolism of xenobiotics in insects and their duplication and upregulation can lead to insecticide resistance. The interaction between P450s and other enzymes like NADPH cytochrome P450 oxidoreductase and cytochrome b5 is complex and can impact metabolic capacity. Recombinant expression of P450s in insect cells with cytochrome b5 can significantly enhance their ability to metabolize certain substrates, but not necessarily insecticides like deltamethrin.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Parasitology
Xixi Li, Shengli Hu, Hongbo Zhang, Haitao Yin, Huan Wang, Dan Zhou, Yan Sun, Lei Ma, Bo Shen, Changliang Zhu
Summary: This study investigated the roles of miR-279-3p and its target gene CYP325BB1 in deltamethrin resistance in Culex pipiens pallens. The findings showed that miR-279-3p suppresses CYP325BB1 expression, reducing deltamethrin resistance and increasing mosquito mortality. These results provide important insights for future mosquito control strategies.
PARASITES & VECTORS
(2021)
Article
Agriculture, Multidisciplinary
Carlos A. G. Rigon, Luan Cutti, Guilherme M. Turra, Enrico Z. Ferreira, Christian Menegaz, Walker Schaidhauer, Franck E. Dayan, Todd A. Gaines, Aldo Merotto
Summary: Herbicide mixtures are commonly used to control a wide range of weeds, including those resistant to specific herbicides. However, the impact of mixtures on the evolution of herbicide resistance through enhanced metabolism is not well understood. In this study, the effects of a fenoxaprop-p-ethyl and imazethapyr mixture on herbicide resistance evolution in Echinochloa crus-galli were evaluated. Results showed that recurrent selection with the mixture led to decreased control and increased resistance to several herbicides. Additionally, it was found that the main contributor to decreased control was fenoxaprop. This study highlights the need for using fully recommended herbicide rates in mixtures to reduce the risk of resistance evolution.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Entomology
Natalie Lissenden, Mara D. Kont, John Essandoh, Hanafy M. Ismail, Thomas S. Churcher, Ben Lambert, Audrey Lenhart, Philip J. McCall, Catherine L. Moyes, Mark J. I. Paine, Giorgio Praulins, David Weetman, Rosemary S. Lees
Summary: Pyrethroids are the main strategy for controlling malaria vectors, but mosquito resistance exists and there are differences in mortality between different pyrethroids. Therefore, rotating between common pyrethroids is not advisable as an insecticide resistance management strategy in areas where resistance exists.
Article
Plant Sciences
Jiankang Xin, Tianmin Che, Xiaolong Huang, Huiqing Yan, Shan Jiang
Summary: In this study, the functions of PpCYP98 in Physcomitrium patens were investigated using transcriptome and metabolome analyses. A total of 5266 differentially expressed genes (DEGs) and 68 differentially abundant secondary metabolites were identified between wild-type and ∆PpCYP98 gametophores. The results showed that PpCYP98 plays vital roles in the pre-lignin synthesis pathway and the synthesis of flavonoids in bryophytes. PpCYP98 also showed systemic effects on metabolisms, including carbohydrate, fatty acid, and hormonal signaling transductions.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Yixi Zhang, Baojun Yang, Zhiming Yang, Lu Kai, Zewen Liu
Summary: Two P450 enzymes, CYP4C62 and CYP6BD12, in Nilaparvata lugens were found to bioactivate chlorpyrifos into its active ingredient chlorpyrifos-oxon in vivo and in vitro. Knockdown of these two genes reduced sensitivity to chlorpyrifos and the formation of chlorpyrifos-oxon in N. lugens. This study revealed a novel mechanism of insecticide resistance due to the reduction in bioactivation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jiang Zhu, Ruinan Qu, Yawei Wang, Ruoyao Ni, Kai Tian, Chan Yang, Mei Li, Michael Kristensen, Xinghui Qiu
Summary: Many insect species have developed various resistance mechanisms in response to insecticide selection. This study focuses on understanding the molecular mechanisms of P450-mediated insecticide resistance using the house fly. The results highlight the common involvement of the CncC pathway in adaptive evolution and provide evidence for parallel evolution in P450-mediated insecticide resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Entomology
Ralf Nauen, Chris Bass, Rene Feyereisen, John Vontas
Summary: Insect cytochrome P450 monooxygenases (P450s) play important physiological roles and are particularly involved in the detoxification of xenobiotics. Recent advances in insect genomics and functional approaches have shed light on the evolution of insect P450s, their regulation mechanisms, and the genetic alterations leading to insecticide resistance. Additionally, studies on the sensitivity of beneficial insects to insecticides have provided valuable knowledge for managing P450-mediated resistance and safeguarding their health.
ANNUAL REVIEW OF ENTOMOLOGY
(2022)
Article
Entomology
Xin-Yeng Leong, Chow-Yang Lee, G. Veera Singham, Alexander Chong Shu-Chien, Richard Naylor, Alexia Naylor, Dini M. Miller, Morgan M. Wilson, David G. Lilly, Stephen L. Doggett
Summary: Modern bed bugs exhibit resistance to multiple insecticide classes, including pyrethroids. The efficacy of pyrethroid-impregnated mattress liners for bed bug management is inconsistent. This study demonstrates that pyrethroid-impregnated mattress liners are unlikely to effectively manage modern bed bug infestations.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Kai Dang, Stephen L. Doggett, Chow-Yang Lee
Summary: This study evaluated the residual performance of two pyrethroid-neonicotinoid mixture formulations on glass and filter paper against eight pyrethroid-resistant strains of tropical bed bugs from Malaysia and Australia. The results showed varying levels of performance for Temprid SC against different strains, while Tandem displayed high performance against all strains. Additionally, the residues of Temprid SC and Tandem showed faster mortality on glass compared to filter paper, and the efficacy of Temprid SC residues declined after one week of aging.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Shu-Ping Tseng, Siavash Taravati, Dong-Hwan Choe, Michael K. Rust, Chow-Yang Lee
Summary: This study used mitochondrial genes and microsatellite markers to analyze the genetic relationship of Formosan subterranean termite infestations in southern California. The results indicate the occurrence of at least three independent introduction events.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Entomology
Chow-Yang Lee, Changlu Wang, Nan-Yao Su
Summary: Bed bugs are important medical and urban pests that preferentially infest low socioeconomic communities. Despite progress in research and control strategies, infestations in poor communities continue to be a reservoir for wider society. This collection of research papers explores bed bug detection, insecticide performance and resistance, nonchemical treatment, and pest management procurement and contracts, aiming to inspire future research on safer, more affordable, and effective control options.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Virology
Chih-Chi Lee, Hung-Wei Hsu, Chun-Yi Lin, Nicolas Gustafson, Kenji Matsuura, Chow-Yang Lee, Chin-Cheng Scotty Yang
Summary: In this study, a transcriptomic database was generated for the yellow crazy ant to identify RNA viruses and characterize their genomes. Multiple virus species were discovered, including the polycipivirus AgrV-3, which showed high genetic diversity and evolved following the quasispecies model. The viral pathogen hotspot of the ant was found in Southeast Asia, consistent with its native range, and multiple virus species were prevalent in field colonies, possibly linked to the ant's social organization.
Article
Entomology
Shawky M. Aboelhadid, Abdel-Azeem S. Abdel-Baki, Sahar M. Gadelhaq, Walid H. Hassan, Lamjed Mansour, Saleh Al-Quraishy, Yoshitaka Kamimura, Chow-Yang Lee, Asmaa A. Kamel
Summary: Based on morphological and molecular data, the earwig samples collected from a bakery in Beni-Suef, Egypt were identified as Marava arachidis. Laboratory findings revealed that this species has the ability to prey on eggs and larvae of the Rhipicephalus annulatus tick. This study provides the first record of M. arachidis in Egypt and demonstrates its predation capability.
Article
Entomology
Jun-Yin Lum, Ming-Chung Chiu, Shu-Ping Tseng, Chin-Cheng Scotty Yang, Chow-Yang Lee
Summary: This study investigated the impact of different intensities of human activity on the distribution of the longlegged ant. The results showed that moderate human activity had a positive effect on the abundance of the ant species, while low and high human activity had less impact.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Cheng-Lung Tsai, Chia-Ning Lu, Hau-You Tzeng, Elliot S. Krafsur, Wu-Chun Tu, Wen-Bin Yeh
Summary: This study used mitochondrial gene analysis to reveal the global genetic structure of the bloodsucking fly, Stomoxys calcitrans. The major divergence occurred 0.32-0.47 million years ago, with subsequent diversifications taking place during 0.13-0.27 million years ago. The study suggests that S. calcitrans originated in the Ethiopian region and has non-monophyletic relationships possibly due to ancestral retention, dispersal with mammals, long-distance migration, and the international livestock industries. The study also highlights the potential veterinary importance of three highly diverged Ethiopian lineages.
MEDICAL AND VETERINARY ENTOMOLOGY
(2023)
Article
Virology
Chih-Ying Kuan, Tsai-Lu Lin, Shan-Chia Ou, Shih-Te Chuang, Jacky Peng-Wen Chan, Ken Maeda, Tetsuya Mizutani, Ming-Pin Wu, Fan Lee, Fang-Tse Chan, Chao-Chin Chang, Rui-Ling Liang, Sue-Fung Yang, Tsung-Ching Liu, Wu-Chun Tu, Hau-You Tzeng, Chia-Jung Lee, Chuen-Fu Lin, Hsu-Hsun Lee, Jhih-Hua Wu, Hsiao-Chien Lo, Kuan-Chieh Tseng, Wei-Li Hsu, Chi-Chung Chou
Summary: Since the discovery of SFTSV in China in 2009, it has quickly spread to other Asian countries. In 2020, Taiwan reported its first discovery of SFTSV in animals and humans. This study found a wide distribution and high prevalence of SFTSV in domestic animals and ticks in Taiwan. Sheep had the highest viral RNA prevalence, followed by beef cattle, goats, and dairy cows. The prevalence in ticks was similar to that in ruminants, but wild animals showed a much lower prevalence. The geographic distribution of positivity was fairly even across northern, central, southern, and eastern Taiwan.
Review
Entomology
John So, Dong-Hwan Choe, Michael K. Rust, John T. Trumble, Chow-Yang Lee
Summary: Selenium is an essential trace element for many organisms but exhibits toxic effects at higher doses. Previous studies have shown negative impacts of selenium exposure on various insect orders, but no clear patterns of toxicity between orders or similarities between species within families. Additional research is needed to determine the potential effects of selenium on beneficial insects and its compatibility with biological control agents. This review explores selenium as a potential insecticide and future research directions.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Benning Le, Hoeun Park, Kathleen Campbell, Michael K. Rust, Chow-Yang Lee, Dong-Hwan Choe
Summary: Due to their mutualistic relationship with plant pests, the Argentine ant is considered a major pest in subtropical fruit orchards and vineyards. Liquid baiting using hydrogel materials has been demonstrated as an effective method to suppress the Argentine ant populations. In this study, boric acid incorporated in a calcium alginate hydrogel effectively killed Argentine ant workers, and the preservative potassium sorbate did not impact the efficacy of boric acid.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Michael K. Rust, Chow-Yang Lee, Ho Eun Park, Kathleen Campbell, Dong-Hwan Choe, Mary Sorensen, Andrew Sutherland, Casey Hubble, Beatriz Nobua-Behrmann, John Kabashima, Shu-Ping Tseng, Linda Post
Summary: The western yellowjacket is an important seasonal pest in the western United States. This study aimed to determine the potential of using the isoxazoline fluralaner as a bait toxicant. Microsatellite genotyping revealed at least 27 different colonies foraging at a single monitoring site. Some colonies disappeared after baiting, and new colonies were detected. Minced chicken and hydrogel baits containing fluralaner significantly reduced foraging yellowjackets. Multiple bait applications will be necessary for long-term control.
Article
Entomology
Shu-Ping Tseng, Lori J. Nelson, Casey W. Hubble, Andrew M. Sutherland, Michael Haverty, Chow-Yang Lee
Summary: Subterranean termites in the Reticulitermes genus are economically important wood-destroying pests in the western United States. However, there is uncertainty regarding the taxonomy and biology of these species. Through genetic analysis, it has been found that there may be at least 5 species of Reticulitermes in California and that the termites in Arizona belong to multiple clades.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Rungarun Tisgratog, Chanikarn Panyafeang, Shao-Hung Lee, Michael K. Rust, Chow-Yang Lee
Summary: We investigated the insecticide resistance profiles of field populations of the German cockroach in central regions of Thailand. The study found resistance to fipronil, deltamethrin, and imidacloprid in the field strains. Synergism studies suggested the involvement of detoxification pathways in the resistance. Field-collected strains also showed resistance to several gel baits.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Shao-Hung Lee, Dong-Hwan Choe, Michael K. Rust, Chow-Yang Lee
Summary: This study investigated the oral toxicity of sucralose on German cockroaches and found that different strains of cockroaches have different tolerances to sucralose. Dehydrated cockroaches were more susceptible to sucralose toxicity, and the treatment also had an impact on gut bacterial composition.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
