Article
Materials Science, Multidisciplinary
Cristina Gila-Vilchez, Mari Carmen Manas-Torres, Oscar Dario Garcia-Garcia, Alfredo Escribano-Huesca, Laura Rodriguez-Arco, Victor Carriel, Ismael Rodriguez, Miguel Alaminos, Modesto Torcuato Lopez-Lopez, Luis Alvarez de Cienfuegos
Summary: Fibrin hydrogels made from human plasma fibrinogen have excellent biocompatibility and biodegradability and are widely used in regenerative medicine. By combining fibrinogen with Fmoc-FF and Fmoc-RGD, we have developed composite hydrogels that have improved mechanical properties compared to pure fibrin gels. These composite gels show excellent biocompatibility in vitro and do not cause any inflammatory response or tissue damage in vivo, making them potential vehicles for drug or growth factor release.
ACS APPLIED POLYMER MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
David Muallah, Jan Matschke, Matthias Kappler, Lysann Michaela Kroschwald, Gunter Lauer, Alexander W. Eckert
Summary: Xerostomia, or dry mouth, is mainly caused by hypofunction of the salivary glands due to various factors. Current treatment methods are not very effective and regenerative medicine using dental pulp stem cells shows promise. Dental pulp stem cells can differentiate into different cell types and have immunomodulatory effects. However, more clinical studies are needed to fully understand their potential for salivary gland regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Engineering, Biomedical
Motaharesadat Hosseini, Karl R. Koehler, Abbas Shafiee
Summary: Current models for generating human skin organs lack appendages, posing a challenge for engineering skin with all components. Researchers are striving to regenerate skin organs with appendages using bioengineering approaches.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Dentistry, Oral Surgery & Medicine
B. Chang, C. Ma, J. Feng, K. K. H. Svoboda, X. Liu
Summary: Researchers explored the effects of biophysical factors on single DPSC polarization using a bioinspired platform, and found that nanofibrous architecture, microisland area, tubular size, and gravity are involved in regulating DPSC polarization. The study showed that nanofibrous architecture, tubular size, and appropriate microisland area are essential for initiating DPSC polarization.
JOURNAL OF DENTAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Priti Gupta, Shilpa Sharma, Shagufta Jabin, Sapana Jadoun
Summary: Regenerative medicine and tissue engineering have great potential in addressing the limitations of traditional medical approaches. Chitosan, as a naturally derived biocompatible biomaterial, has played a significant role in tissue regeneration. It has unique properties and can be applied in various fields, such as agriculture, nutraceuticals, biomedicine, and food. Chitosan is also an important material for developing next-generation hydrogels and bio-scaffolds. Furthermore, chitosan can be easily modified to enhance its mechanical strength, biodegradability, and controlled release of bioactive molecules. Blending chitosan with other polymers or incorporating nanoparticles expands its possibilities in tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Pharmacology & Pharmacy
Mohammad Samiei, Elaheh Dalir Abdolahinia, Marziyeh Fathi, Jaleh Barar, Yadollah Omidi
Summary: In this study, chitosan-based hydrogels were used to improve the proliferation and differentiation of dental pulp stem cells (DPSCs), with CS/GN hydrogel showing better features in enhancing DPSCs viability and osteogenic differentiation.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Julia K. Bar, Anna Lis-Nawara, Piotr Grzegorz Grelewski
Summary: The secretome derived from dental pulp stem cells and human exfoliated deciduous teeth has therapeutic potential in regenerating bone, cartilage, and nerve tissue. By secreting bioactive factors, these cells can modulate processes such as neuroprotection, anti-inflammatory, antiapoptotic, and angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Qi Feng, Dingguo Li, Qingtao Li, Xiaodong Cao, Hua Dong
Summary: This mini-review summarizes the fabrication strategies, unique characteristics, and applications of microgel assembly in tissue engineering and regenerative medicine, with a focus on 3D printing. The challenges for further applications of microgel assembly are also discussed.
BIOACTIVE MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Cristina Gila-Vilchez, Mari Carmen Manas-Torres, Oscar Dario Garcia-Garcia, Alfredo Escribano-Huesca, Laura Rodriguez-Arco, Victor Carriel, Ismael Rodriguez, Miguel Alaminos, Modesto Torcuato Lopez-Lopez, Luis Alvarez de Cienfuegos
Summary: Fibrin hydrogels made by self-assembly of fibrinogen from human plasma are highly biocompatible and biodegradable. However, they have limited mechanical properties and are expensive to obtain. To address these issues, composite hydrogels made of fibrinogen, Fmoc-FF, and Fmoc-RGD were prepared. These composite gels exhibited improved mechanical properties compared to pure fibrin gels and showed excellent biocompatibility ex vivo. In vivo experiments showed that these gels do not cause inflammation or tissue damage and are completely resorbed, making them suitable for cell, drug, or growth factor release vehicles.
ACS APPLIED POLYMER MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Ella Ohlsson, Kerstin M. Galler, Matthias Widbiller
Summary: Efforts in tissue engineering to repair damaged dental pulp tissue have shown positive results, but clinical measures cannot distinguish between true regeneration and repair. Therefore, research models are crucial in understanding treatment outcomes and advancing dental pulp regeneration. This review provides information on various in vitro and in vivo models, discusses the differences between monolayer and three-dimensional cell cultures, presents a new in vivo transplantation model for dental pulp regeneration, and introduces different animal models used for in vivo research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Iriczalli Cruz-Maya, Rosaria Altobelli, Marco Antonio Alvarez-Perez, Vincenzo Guarino
Summary: There is growing interest in using micro-sized hydrogels to mimic cell niche structure and selected functionalities for tissue regeneration. In this study, bioactive composite microgels were optimized through electrohydrodynamic atomization (EHDA) for dentin-pulp complex regeneration. The mineralized microgels were successfully used to support in vitro osteogenesis, serving as an interesting model for studying dental tissue regeneration.
Review
Biochemistry & Molecular Biology
Nikita Voloshin, Pyotr Tyurin-Kuzmin, Maxim Karagyaur, Zhanna Akopyan, Konstantin Kulebyakin
Summary: Immortalized cells are increasingly used in practical medicine due to their advantages, such as the ability to produce larger amounts of cells and use them for longer periods of time. However, they also have drawbacks, like the possibility of malignant transformation or major phenotype change. This review discusses ways to overcome these barriers and highlights specific fields of application where these concerns are minimal.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Zhuo Xie, Zongshan Shen, Peimeng Zhan, Jiayu Yang, Qiting Huang, Shuheng Huang, Lingling Chen, Zhengmei Lin
Summary: Treatment of pulpal and periapical diseases faces challenges, but significant progress has been made in achieving true pulp tissue regeneration through technologies such as stem cells, biomaterials, and growth factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Mai Takeshita-Umehara, Reiko Tokuyama-Toda, Yusuke Takebe, Chika Terada-Ito, Susumu Tadokoro, Akemi Inoue, Kohei Ijichi, Toshio Yudo, Kazuhito Satomura
Summary: Dental pulp stem cells (DPSCs) are highly valuable for regenerative medicine due to their proliferative potential and multipotency. A new cryopreservation method (NCM) was developed to collect DPSCs by freezing the tissue and collecting the cells after thawing. An improved method was developed to efficiently collect and preserve DPSCs by culturing dental pulp tissue with cell culture inserts, resulting in vertical cell migration and localization near the membrane. The underlying molecular mechanism involves the predominance of SDF1 in cell migration.
Review
Biochemistry & Molecular Biology
Victor J. Costela-Ruiz, Lucia Melguizo-Rodriguez, Chiara Bellotti, Rebeca Illescas-Montes, Deborah Stanco, Carla Renata Arciola, Enrico Lucarelli
Summary: The success of regenerative medicine depends on the appropriate selection of mesenchymal stem cell (MSC) sources, as they influence the regenerative milieu. MSCs from bone marrow, adipose tissue, and dental tissue are all potential candidates for oral and dental regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Maria Clara Bertolini Botelho, Luciano Tavares Angelo Cintra, Cristiane Cantiga da Silva, Giseli Mitsuy Kayahara, Rosani Belzunces Pereira, Mylena Fernanda Oliveira Santos, Glauco Issamu Miyahara, Eder Ricardo Biasoli, Sandra Helena Penha Oliveira, Daniel Galera Bernabe
Summary: This study evaluated the influence of early life stress on the severity of apical periodontitis in Wistar rats. The results showed that early life stress exacerbates the inflammatory process and increases periapical bone resorption.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Alexandre Henrique dos Reis-Prado, Sabrina de Castro Oliveira, Juliana Goto, Gerluza Aparecida Borges Silva, Luciano Tavares Angelo Cintra, Ricardo Alves de Mesquita, Raphael Escorsim Szawka, Antonio Paulino Ribeiro-Sobrinho, Francine Benetti
Summary: EDTA irrigation was found to improve the formation of newly formed intracanal connective tissue after REP in immature rat molars. However, EDTA did not have an influence on cementum-like tissue formation, apical closure, inflammatory infiltrate, and maturation of collagen fibers.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Bianca Elvira Belardi, Maria Sara de Lima Coutinho Mattera, Thais Veronica Saori Tsosura, Rodrigo Martins Dos Santos, Cristiane Cantiga-Silva, Luciano Tavares Angelo Cintra, Fernando Yamamoto Chiba, Lara Teschi Bravo, Nubia Ramos Carvalho, Ana Carolina Nascimento Carnevali, Renan Akira Fujii de Oliveira, Edilson Ervolino, Doris Hissako Matsushita
Summary: Maternal periodontal disease and apical periodontitis are associated with insulin resistance, alterations in insulin signaling, and inflammatory pathways in the gastrocnemius muscle of adult offspring, leading to worsened health.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ana Maria Veiga Vasques, Ana Claudia Rodrigues da Silva, Carlos Roberto Emerenciano Bueno, Marina Tolomei Sandoval Cury, Edilson Ervolino, Luciano Tavares Angelo Cintra, Eloi Dezan Junior
Summary: The aim of this study was to evaluate the effects of cigarette smoke inhalation on inflammation, pro-inflammatory mediators and haematological parameters in rats with induced apical periodontitis. The results showed that cigarette smoke inhalation aggravated the inflammatory response in rats with apical periodontitis, causing increased levels of pro-inflammatory cytokines and changes in haematological parameters.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Henrique R. Matheus, Henrique Hadad, Joao L. G. C. Monteiro, Toru Takusagawa, Fugui Zhang, Qingsong Ye, Yan He, Ivy A. Rosales, Youssef Jounaidi, Mark A. Randolph, Fernando P. S. Guastaldi
Summary: This study investigated the effects of Gelatin-methacryloyl (GelMA) hydrogel scaffold loaded with dental pulp stem cells (DPSCs) and vascular endothelial growth factor (VEGF) on the healing of skin wounds in rats. The results showed that the GelMA+DPSCs and GelMA+DPSCs+VEGF groups exhibited complete epithelialization of the wounds at 2 weeks, and the epidermis in these groups was comparable to the empty defect and GelMA groups at 4 weeks. Furthermore, the GelMA+DPSCs+VEGF group demonstrated a decrease in cellularity, an increase in collagen fiber density, and higher expression of the KER 10 gene compared to the other groups. The expression of Col1 A1 and TGFb-1 did not show statistically significant differences over time and among the groups. In conclusion, GelMA scaffolds loaded with DPSCs and VEGF accelerated the re-epithelialization of skin wounds.
JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Juliano Milanezi de Almeida, Daniela Pereira de Sa, Elisa Mara de Abreu Furquim, Henrique Rinaldi Matheus
Summary: This case report describes a modified one-stage technique for treating soft tissue defects around dental implants in the esthetic zone. The technique successfully covered the exposed implant and improved the soft tissue phenotype.
CLINICAL ADVANCES IN PERIODONTICS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Renato de Toledo Leonardo, Carlos Garcia Puente, Fabio Luiz Camargo Villela Berbert, Gisele Faria, Celso Kenji Nishiyama, Fernando Accorsi Orosco, Gladiston Wiliam Lobo Rodrigues, Ana Paula Fernandes Ribeiro, Luciano Tavares Angelo Cintra, Martin Trope
Summary: This study aimed to evaluate the antimicrobial efficacy of laser ablation (LA) therapy with indocyanine green (ICG) in root canal treatment. Results showed that LA therapy with ICG significantly increased microbial reduction in root canals regardless of instrumentation sizes or the irrigation solution used.
JOURNAL OF ENDODONTICS
(2023)
Article
Engineering, Biomedical
Henrico Badaoui Strazzi-Sahyon, Henrique Augusto Banci, Fernanda Santos de Melo, Lucas Silva Soares, Rubens Spin-Neto, Marco Antonio Hungaro Duarte, Luciano Tavares Angelo Cintra, Paulo Henrique dos Santos, Gustavo Sivieri-Araujo
Summary: This in vitro study evaluated the influence of antimicrobial photodynamic therapy (aPDT), using methylene blue (MB) as photosensitizer (PS) and calcium hydroxide (CH) as intracanal medication on adhesive bond strength, sealing, and integrity of the luting interface of glass-fiber posts to different thirds of endodontically treated root canal dentin. The results showed that aPDT combined with calcium hydroxide can achieve satisfactory adhesive bond strength, sealing, and integrity of the adhesive interface at any intra-radicular depth.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Bethania Camargo Pinheiro, Tiago Novaes Pinheiro, Milena Gomes Melo Leite, Roberto Brandao Garcia, Norberti Bernardineli, Flavio Duarte Faria, Luciano Tavares Angelo Cintra, Clovis Monteiro Bramante
Summary: The effect of ELVAX polymer subgingival implants with echistatin peptide on incisor reimplanted tooth in rats was investigated. The study involved forty-two male Wistar rats divided into a group treated with echistatin (E group) and a control group (C group). The results showed that the presence of inflammatory resorption was significantly higher in the C group at 30 and 60 min extra-alveolar time and the 15-day postoperative period compared to the E group. Dental ankylosis was more prevalent in the E group at 30 min extra-alveolar time and the 15-day postoperative period. However, in the 60 min extra-alveolar time and 60-day postoperative period, dental ankylosis was more prevalent in the C group. The use of ELVAX subgingival implants with echistatin showed therapeutic potential in preventing resorption after replantation of maxillary incisors in rats.
Article
Multidisciplinary Sciences
Juliano Milanezi de Almeida, Nathalia Januario de Araujo, Henrique Rinaldi Matheus, Elisa Mara de Abreu Furquim, Bianca Rafaeli Piovezan, Luiz Guilherme Fiorin, Edilson Ervolino
Summary: This study aimed to evaluate the effectiveness of hand debridement (HD) versus ultrasonic dental scaler (UDS) for the treatment of experimental periodontitis (EP) in rats. The results showed that HD resulted in higher percentage of bone in the furcation (PBF) and lower expression of tartrate-resistant acid phosphatase (TRAP) compared to UDS. HD also increased the expression of osteocalcin (OCN) over time.
Article
Dentistry, Oral Surgery & Medicine
Henrique Hadad, Henrique Rinaldi Matheus, Jason Evan Chen, Youssef Jounaidi, Fernando Pozzi Semeghini Guastaldi
Summary: Recent studies have shown that bisphosphonates not only affect osteoclasts but also impact osteoblasts. This study aimed to evaluate the effects of different concentrations of Zoledronic acid (ZA) on the osteogenic differentiation of human Bone Marrow Stem Cells (hBMSCs) in vitro. The results showed that high concentrations of ZA can impair cell metabolism, mineralization, alkaline phosphatase activity, and downregulate RUNX2 expression.
JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Fred Lucas Pinto Oliveira, Henrique Rinaldi Matheus, Edilson Ervolino, Vivian Cristina Noronha Novaes, Bianca Rafaeli Piovezan, Elisa Mara de Abreu Furquim, Luiz Guilherme Fiorin, Juliano Milanezi de Almeida
Summary: Adjunctive therapy with local sodium alendronate prevents alveolar bone loss and improves the histopathological features of the periodontal tissues following scaling and root planing in male rats with experimental periodontitis receiving anticancer chemotherapy with 5-Fluorouracil.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ana Claudia Rodrigues da Silva, Ana Maria Veiga Vasques, Carlos Roberto Emerenciano Bueno, Edilson Ervolino, Luciano Tavares Angelo Cintra, Claudia Cristina Biguetti, Mariza Akemi Matsumoto, Eloi Dezan-Junior
Summary: This study evaluated the effects of cigarette smoke inhalation on apical periodontitis in rats. The results showed that cigarette smoke worsened the condition and led to a pro-inflammatory immune response.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Claudia Azucena Palafox- Sanchez, Alvaro Cruz, Diana Celeste Salazar-Camarena, Luis Gerardo Gascon, Luciano Tavares Angelo Cintra, Jose Francisco Munoz-Vale, Samuel Garcia-Arelano, Carlos Estrela, Paula Annahi Menchaca-Tapia
Summary: This study investigated the changes in systemic cytokine levels in patients with acute apical abscesses (AAA), and found that patients with AAA had increased serum levels of TNF-a, IL-6, and IL-10. Antibiotic treatment was associated with a decrease in IL-6 and IL-10 levels, while TNF-a levels decreased after antibiotic and endodontic treatment.
JOURNAL OF ENDODONTICS
(2023)
Review
Geriatrics & Gerontology
Sara Pai, Henrique R. Matheus, Fernando P. S. Guastaldi
Summary: Periodontitis, resulting from dysbiosis of the oral microbiome, is a common condition affecting a large percentage of older adults in the United States. It is associated with various systemic inflammatory disorders and comorbidities, some of which overlap with immunotherapy-associated toxicities. The impact of periodontal disease on the response rates and tolerance to cancer immunotherapy remains uncertain. Further research is needed on the influence of other microorganisms causing periodontal disease. Addressing periodontitis in elderly cancer patients could have implications for the effectiveness and tolerability of immunotherapy.
LANCET HEALTHY LONGEVITY
(2023)