Article
Chemistry, Physical
Yingfeng Wang, Maoli Yin, Zhipeng Ma, Yang Wang, Wei Li, Huang Hu, Xin Hong
Summary: In this study, N-halamines were introduced into mesoporous silica to enhance its surface area retention rate and water treatment properties. The modified mesoporous silica showed effective antibacterial properties against E. coli and S. aureus, and exhibited good adsorption abilities for organic dyes. These findings suggest that the modified mesoporous silica has great potential for applications in wastewater purification.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Chemistry, Physical
Mengnan Yu, Mingming Liu, Liping Zhang, Min Li, Yuanyuan Hou, Dong Wang, Shaohai Fu
Summary: In this study, a lubricant-grafted slippery surface (LGSS) was prepared by grafting vinyl-terminated polydimethylsiloxane (Vi-PDMS) onto sulfhydryl-modified hollow mesoporous silica (SH-HMS) through a thiol-ene click reaction, exhibiting excellent antifouling and self-repairing properties. This newly developed strategy for constructing liquid-repellent coatings has the potential to advance interfacial antifouling materials.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Analytical
Ying Wang, Ji-Kai Chen, Ling-Xiao Xiong, Bang-Jin Wang, Sheng-Ming Xie, Jun-Hui Zhang, Li-Ming Yuan
Summary: This study demonstrates the first immobilization of a chiral porous organic cage on thiol-functionalized silica to prepare novel chiral stationary phases for high-performance liquid chromatography. The prepared CSPs exhibited excellent enantioselectivity for the resolution of various racemates. The importance of the cationic imidazolium spacer for chiral separation was also demonstrated. The chiral POCs showed good chiral recognition complementarity with the two most popular commercial chiral columns.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
You -Ping Zhang, Ling-Xiao Xiong, Ying Wang, Kuan Li, Bang-Jin Wang, Sheng-Ming Xie, Jun -Hui Zhang, Li -Ming Yuan
Summary: Polyimine macrocycles are a new class of organic macrocycles with cyclic structures and well-defined molecular cavities. They have attracted considerable research interest in molecular recognition and separation. In this study, a chiral polyimine macrocycle was bonded on thiol-functionalized silica gel using thiol-ene click chemistry to prepare a chiral stationary phase for high-performance liquid chromatography. The fabricated column showed excellent chiral separation capability under both normal-phase and reversed-phase conditions, and exhibited good selectivity towards positional isomers. The study indicates the promising potential of this type of chiral macrocycles for enantioseparation in HPLC.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Inorganic & Nuclear
Joanna K. Szymkowiak, Christopher M. Walters, Wadood Y. Hamad, Mark J. MacLachlan
Summary: In this paper, we functionalize chiral nematic mesoporous materials with different functional groups on the surface by incorporating vinyl groups and using the thiol-ene click reaction. The materials exhibit tunable hydrophobicity, enabling the detection of mixtures of organic solvents in water and promising ion sequestration potential.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Kezi Cheng, Alex Chortos, Jennifer A. Lewis, David R. Clarke
Summary: Covalent adaptive networks combine the advantages of cross-linked elastomers and dynamic bonding in a single system. A simple one-pot method to prepare thiol-ene elastomers that exhibit reversible photoinduced switching from an elastomeric gel to fluid state is demonstrated. This behavior can be generalized to different composition and chemistries and allows for self-healing, remolding, and reversible adhesion.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Polymer Science
D. A. Lokteva, Yu N. Kononevich, M. N. Temnikov, P. A. Nezhnyy, E. E. Kim, D. A. Khanin, G. G. Nikiforova, A. M. Muzafarov
Summary: In this study, a series of new cross-linked polysiloxane polymers were successfully prepared by UV-induced hydrothiolation click-reaction using functionalized cyclo- and oligosiloxane precursors. The physico-mechanical properties of these polymers were investigated, showing that their mechanical properties and thermal stability can be fine-tuned by changing the structure of the initial vinyl precursor.
Article
Nanoscience & Nanotechnology
Tingting Zhang, Sha Liu, Xiaozhi Zhang, Jingde Gao, Hong Yu, Qian Ye, Shujuan Liu, Weimin Liu
Summary: A facile and efficient postsynthetic strategy was proposed to modify two-dimensional COFs by covalently bonding commercial lubricating molecules, resulting in DDP@TD-COF with exceptional dispersion stability and friction reduction abilities. Addition of a trace amount of DDP@TD-COF significantly decreases friction coefficient, reduces wear volume loss, and enhances load carrying ability, showing promising potential for practical applications as nanoadditives.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Polymer Science
Baoliang Wang, Lili Xing, Tieling Xing, Guoqiang Chen
Summary: A superhydrophobic fabric was synthesized by modifying the fiber's surface with dopamine-containing hydroxyl functional groups. This fabric has a compact and regular micro-nano rough structure based on POSS and mercaptans, providing stable fastness and durability, as well as high resistance to various environmental factors. It can be used for self-cleaning, oil-water separation, and has wide applications in the coating industry.
Article
Nanoscience & Nanotechnology
Kavya Ganabady, Nicola Contessi Negrini, Jacob C. Scherba, Brandon M. Nitschke, Morgan R. Alexander, Kyle H. Vining, Melissa A. Grunlan, David J. Mooney, Adam D. Celiz
Summary: In this study, we employed a high-throughput screening strategy to rapidly identify a biocompatible bone adhesive with high bond strength. Through thiol-ene click chemistry, a library of polymers was synthesized and a copolymer (1-S2-FT01) composed of TATATO and PETMP was identified as the most suitable candidate. This copolymer exhibited favorable mechanical properties, supported hMSC adhesion, adhered to bone tissue with similar strength to commercially available bone glues, and facilitated osteogenic differentiation of hMSCs and attachment of polymer scaffolds to bone tissue, making it an ideal alternative bone adhesive for orthopedic surgery.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Materials Science, Multidisciplinary
Hang Shen, Siyi Li, Huagui Zhang
Summary: In this study, urushiol-urethane prepolymers were synthesized and used as the foundation for transparent and thick lacquer film preparation through a fast and green thiol-ene click reaction. The resulting films exhibit excellent properties such as flexibility, transparency, and renewable characteristics. The cross-linking density, modulus, and hydrophobicity of the films can also be adjusted. Furthermore, the method can be applied to the preparation of other materials.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Polymer Science
Yuhan Li, Ziyu Liu, Xiaoran Liu, Feng Gao, Jue Cheng, Junying Zhang
Summary: Polyurethane (PU) is a commonly used polymer material, but its hydrophilic nature poses challenges to its application in flexible electronic displays. In this study, fluorinated chain extenders were introduced into PU to reduce surface energy and improve surface roughness. This resulted in excellent hydrophobicity and oleophobicity, as well as enhanced mechanical properties through physical cross-linking points.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Engineering, Environmental
Peixin Tang, Gang Sun
Summary: Daylight-activated detoxifying nanofibrous membranes efficiently detoxify 1,3-dichloropropene (1,3-D) through a thiol-ene click reaction under daylight irradiation. The detoxification process is initiated and sustained by light-induced cascade chemical attacks of thiyl radicals, showing rapid and massive detoxification effects. These membranes also demonstrate a detoxification tailing effect in the absence of light sources, making them effective for personal protective equipment.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Engineering, Environmental
Puyou Jia, Meghan E. Lamm, Ye Sha, Yufeng Ma, Leman Buzoglu Kurnaz, Yonghong Zhou
Summary: A green and sustainable strategy is developed for the synthesis of methacrylic monomers and polymer networks using biobased eugenol. The properties of these biobased polymer networks can be tuned by changing the types of eugenol-based monomers and thiol crosslinkers. These networks can be chemically degraded into carboxylic acids and thermally degraded into CO and CO2, offering a pathway to degradable sustainable polymers and materials.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Rui-Xue Liang, Qi-Yu Ma, Tuan-Xiu Xiang, You-Ping Zhang, Ya-Nan Gong, Bin Huang, Bang-Jin Wang, Sheng-Ming Xie, Jun-Hui Zhang, Li-Ming Yuan
Summary: A chiral pillar[3]trianglimine was developed and bonded to thiolated silica to create a novel chiral stationary phase for enantioseparation in high-performance liquid chromatography. The performance of the fabricated chiral stationary phase was evaluated by separating various racemic compounds, and it showed good separation complementarity compared to commonly used chiral columns. The new chiral stationary phase exhibited good repeatability, stability, and column-to-column reproducibility, making it a promising candidate for chiral separation in high-performance liquid chromatography.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Endocrinology & Metabolism
Rosemarie Krug, Laura Beier, Michael Laemmerhofer, Manfred Hallschmid
Summary: This study investigates the cognitive impact of estradiol and insulin in male individuals. The results show that estradiol enhances creativity and verbal memory in young men, while insulin's cognitive impact in young men is limited.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Chemistry, Analytical
Peng Li, Meinrad Gawaz, Madhumita Chatterjee, Michael Laemmerhofer
Summary: In this study, an improved method for detecting inositol phosphates was established using a labeling methylation strategy. This method was successfully applied to biological samples, providing efficient separation and quantification of inositol phosphate metabolites.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Gioacchino Luca Losacco, Heather Wang, Imad A. Haidar Ahmad, Jimmy DaSilva, Alexey A. Makarov, Ian Mangion, Francesco Gasparrini, Michael Laemmerhofer, Daniel W. Armstrong, Erik L. Regalado
Summary: Enantioselective chromatography, especially with the development of a multicolumn UHPLC screening workflow, has shown high efficiency in enantioseparations with minimal manual intervention and high success rate. This approach lays the foundation for streamlined development of ultrafast enantioseparations applied to high-throughput enantiopurity analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Matthias Olfert, Stefanie Baeurer, Marc Wolter, Stephan Buckenmaier, Edmundo Brito-de la Fuente, Michael Laemmerhofer
Summary: This research developed a comprehensive 2D-LC method coupled with inline DAD-UV and ESI-QTOF-MS/MS detection for separating conjugated polyunsaturated fatty acid isomers and structurally related compounds. By combining chiral and reversed-phase columns, advanced structural annotation of unknown compounds and differentiation of positional isomers of oxidized lipids can be achieved.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Min Su, Kristian Serafimov, Peng Li, Cornelius Knappe, Michael Laemmerhofer
Summary: The chromatographic behavior of mixed-mode and hydrophilic interaction liquid chromatography (HILIC) was assessed in this study for the separation of metabolites from glycolysis and pentose phosphate pathways. Optimization of chromatographic conditions allowed for selective targeted analysis of glycolytic phosphorylated carbohydrate metabolites using liquid chromatography with tandem mass spectrometry (MS/MS). The separation of pentose phosphate isomers was achieved using mixed-mode HILIC/SAX and HILIC BEH Amide columns. HILIC-MS/MS showed challenges in separating hexose monophosphate metabolites, but the BEH Amide column provided the best results for this separation.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Christian Geibel, Matthias Olfert, Cornelius Knappe, Kristian Serafimov, Michael Laemmerhofer
Summary: This work presents the application of targeted UHPLC-tandem mass spectrometry methods for the chiral separation of anteiso- methyl branched fatty acids (aiFAs). The methods involve precolumn derivatization and chiral separation. The study not only determines the absolute configuration of fatty acids, but also utilizes polysaccharide columns for structural elucidation of natural compounds.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Feiyang Li, Shenkai Chen, Sylwia Studzinska, Michael Laemmerhofer
Summary: Ion-pairing reversed-phase liquid chromatography (IP-RPLC) is commonly used for the analysis of nucleic acid related compounds. However, the high concentration of ion-pairing reagent in IP-RPLC is not suitable for coupling with mass spectrometry (MS). This study explored the use of a PBT-bonded silica-based stationary phase for the separation of Patisiran without the need for ion-pairing reagents. The method allowed for MS and MS/MS characterization without contamination or related issues.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Medicinal
Teodor Dimitrov, Athina Anastasia Moschopoulou, Lennart Seidel, Thales Kronenberger, Mark Kudolo, Antti Poso, Christian Geibel, Pascal Woelffing, Daniel Dauch, Lars Zender, Dieter Schollmeyer, Juergen Bajorath, Michael Forster, Stefan Laufer
Summary: The ATM kinase is an important regulator of the cellular response to DNA double-strand breaks and is considered a promising target in cancer treatment. This study introduces a new class of specific benzimidazole-based ATM inhibitors with high potency against the isolated enzyme and favorable selectivity within related kinases. These inhibitors show strong enzymatic and cellular activities, as well as promising pharmacokinetic properties and selectivities within the PIKK and PI3K families.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Ulrich Woiwode, Adrian Sievers-Engler, Michael Laemmerhofer
Summary: In this work, new polymeric chiral stationary phases were prepared and compared with brush-type chiral stationary phases. The polymeric CSPs showed similar enantioselectivity but lower chromatographic efficiencies compared to the brush phases. Oxidation of the polymeric CSPs reduced retention times and allowed for lower counterion concentrations in liquid chromatography-electrospray ionization-mass spectrometry applications. In addition, cryogenic cooling of the column enabled baseline separation of estradiol.
Article
Biochemical Research Methods
Xiaoqing Fu, Nourhane Hafza, Friedrich Goetz, Michael Laemmerhofer
Summary: In this study, seven commercially available UHPLC columns were systematically evaluated for their retention and isomer selectivity of branched chain fatty acids (BCFAs). It was found that IG-U column showed the best isomer selectivity for short and medium-chain BCFA isomers, while RP C18 column had good selectivity for long-chain BCFA isomers. Furthermore, an untargeted isomer-selective BCFA profiling method was developed using UHPLC-ESI-MS/MS without pre-column derivatization.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Feiyang Li, Ryan Karongo, Despoina Mavridou, Jeannie Horak, Adrian Sievers-Engler, Michael Laemmerhofer
Summary: Enantioselective amino acid analysis is an important technique in pharmaceutical, biomedical, and food sciences. However, simultaneously analyzing all chiral proteinogenic amino acids using a single method with one column and one condition is still challenging. In this study, an enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS) assay using Chiralpak QN-AX as a chiral column was developed, enabling efficient separation of D- and L-amino acids.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Feiyang Li, Shenkai Chen, Sylwia Studzinska, Michael Laemmerhofer
Summary: With the increasing use of nucleic acid-based pharmaceuticals, efficient analytical methods are needed for synthetic oligonucleotides. Two-dimensional chromatography techniques, including orthogonal chromatographic methods, are becoming more relevant due to the complexity of the oligonucleotides. In this study, a new polybutylene terephthalate-based stationary phase was tested for the analysis of siRNA, showing higher orthogonality and improved resolution for peak purity evaluation.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Medicinal
Sebastian Vaas, Markus O. Zimmermann, Dieter Schollmeyer, Jason Stahlecker, Marc U. Engelhardt, Janosch Rheinganz, Bernhard Drotleff, Matthias Olfert, Michael La''mmerhofer, Markus Kramer, Thilo Stehle, Frank M. Boeckler
Summary: This article highlights the usefulness of CF2X (X = Cl, Br, or I) moieties as an orthogonal principle to the established (hetero)arylhalides. The chemical/metabolic stability, logP/solubility, and crystal structures of compounds bearing CF2X groups are studied. The interactions, conformational flexibilities, and XB donor capacities of CF2X moieties are analyzed. Furthermore, the implementation of CF2X acetamides in molecular design and their binding modes in JNK3 are investigated.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ryan Karongo, Jeannie Horak, Michael Laemmerhofer
Summary: This work presents a comprehensive achiral x chiral two-dimensional liquid chromatography separation method for enantioselective amino acid analysis. It utilizes flow splitting and a multi-detector approach for volumetric flow reduction and efficient ionization. The method overcomes limitations of chiral columns and can be used for complex sample mixtures. It has been successfully applied to control the stereointegrity of the therapeutic peptide octreotide.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Kristian Serafimov, Yuesra Aydin, Michael Laemmerhofer
Summary: In this study, a fast-targeted liquid chromatography-tandem mass spectrometry method was proposed for accurate analysis of metabolites from the glutathione pathway. The addition of N-Ethylmaleimide reagent to the extraction solvent quickly stabilized the thiol-redox state. This method demonstrated high throughput analysis, sensitivity, and accuracy.
JOURNAL OF SEPARATION SCIENCE
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)