Preparation of chitosan-modified magnetic Schiff base network composite nanospheres for effective enrichment and detection of hippuric acid and 4-methyl hippuric acid

Chitosan-modified magnetic Schiffbase network composite nanospheres (Fe3O4 @SNW@Chitosan) were prepared for the enrichment and detection of hippuric acid (HA) and 4-methyl hippuric acid (4-MHA) via magnetic solid phase extraction (MSPE) connected with HPLC. The SNW was one of the covalent organic framework, which constructed through covalent bonds, shown comprising solvent stability, low density and accessible pores. The obtained Fe3O4 @SNW@Chitosan has many merits as a magnetic sorbent, including a hydrophilic surface, uniform pore size, unique ordered channel structure, and superparamagnetism. The favourable linearity of this MSPE-HPLC method was in the range of 1-1000 mu g L-1, and LODs of HA and 4-MHA were 0.3 mu g L- 1 and 0.2 mu g L-1, respectively. The recoveries in urine samples were range from 95.3 to 109.0 % with the RSD less than 9.6 %. When employed for the enrichment of HA and 4-MHA, Fe3O4@SNW@Chitosan exhibited great potential as a candidate for preconcentration. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

The Chitosan-modified magnetic Schiffbase network composite nanospheres Fe3O4 @SNW@Chitosan were successfully used for the enrichment and detection of HA and 4-MHA via MSPE-HPLC method, showing favorable linearity and low LODs. The application in urine samples demonstrated excellent recoveries and stability of the method.