Article
Biochemistry & Molecular Biology
Dmitry K. Sokolov, Oleg B. Shevelev, Anna S. Khotskina, Alexandra Y. Tsidulko, Anastasia V. Strokotova, Galina M. Kazanskaya, Alexander M. Volkov, Evgenii E. Kliver, Svetlana V. Aidagulova, Evgenii L. Zavjalov, Elvira V. Grigorieva
Summary: Glioblastoma (GB) is an aggressive cancer with a high chance of recurrence, and the effects of chemotherapy drugs on heparan sulfate (HS) in brain tissue are still unknown.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
John R. Couchman
Summary: Cell surface proteoglycans, such as syndecans, play crucial roles in regulating cell behavior, including interactions with extracellular matrix components and mediating proliferation, adhesion, and migration. In tumors, changes in the levels and distribution of syndecan-1 can have implications for prognosis, with loss of membrane staining and abnormal cytoplasmic or nuclear staining often indicating poor outcomes. Targeting syndecan-1 with antibody-toxin conjugates may hold promise for clinical applications in both myeloma and some carcinomas.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ayumu Mubuchi, Shoo Katsumoto, Makoto Tsuboi, Hideaki Ishikawa, Yoshihiro Nomura, Kyohei Higashi, Shinji Miyata
Summary: Chondroitin sulfate and heparan sulfate are sulfated glycosaminoglycan chains with different levels of sulfation and disaccharide compositions. The sulfated glycosaminoglycans from Perna canaliculus show potential as biomaterials for studying the structure-function relationships.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Imlirenla Pongener, Conor O'Shea, Hannah Wootton, Michael Watkinson, Gavin J. Miller
Summary: This review focuses on the chemical approaches and methodology improvements for the synthesis of heparin and heparan sulfate since 2015, including advances in accessing the pentasaccharide anticoagulant and targets for heparan sulfate synthesis. It also discusses key building block synthesis, oligosaccharide construction, chemical sulfation techniques, and technological improvements to traditional solution-phase synthesis approaches.
Article
Medicine, Research & Experimental
Mausam Kalita, Javier Villanueva-Meyer, Yuki Ohkawa, Chakrapani Kalyanaraman, Katharine Chen, Esraa Mohamed, Matthew F. L. Parker, Matthew P. Jacobson, Joanna J. Phillips, Michael J. Evans, David M. Wilson
Summary: Aberrant biosynthesis of GAGs in glioma contributes to malignant growth, and disrupting GAGs with small molecule inhibitors shows potential therapeutic benefits in blocking rogue signaling and proliferation in glioblastoma.
MOLECULAR PHARMACEUTICS
(2021)
Article
Microbiology
Carla Martin, Helena Ordiales, Francisco Vazquez, Marta Pevida, David Rodriguez, Jesus Merayo, Fernando Vazquez, Beatriz Garcia, Luis M. Quiros
Summary: This study found that cell surface glycosaminoglycans (GAGs) are involved in the adhesion of acne-related bacteria to skin cells, with different bacteria affecting different cell types. Bacterial adherence also led to changes in gene expression in skin cells, affecting the structure of GAG chains.
Review
Oncology
Catarina Marques, Celso A. Reis, Romain R. Vives, Ana Magalhaes
Summary: Heparan Sulfate Proteoglycans (HSPGs) play important roles in orchestrating cellular events in physiology and pathology by modulating cell signaling networks and cell interactions. The structural features of HS chains are crucial for the functions of HSPGs, influencing protein interactions and signaling. The tightly controlled biosynthetic pathway of HS leads to structural diversity, which is differently regulated in various tissues, developmental stages, and pathologies such as cancer.
FRONTIERS IN ONCOLOGY
(2021)
Review
Polymer Science
Qingchi Wang, Lianli Chi
Summary: This review investigates the structural alterations and roles of GAGs/PGs in various diseases, and discusses their potential in disease diagnosis, monitoring, prognosis, and drug development.
Article
Biochemistry & Molecular Biology
Helena Crijns, Lowie Adyns, Eva Ganseman, Seppe Cambier, Eline Vandekerckhove, Noemie Poertner, Lotte Vanbrabant, Sofie Struyf, Tanja Gerlza, Andreas Kungl, Paul Proost
Summary: This study investigated the interactions between glycosaminoglycans (GAGs) and proteins, as well as the structural requirements for binding. Through peptide design and experiments, it was found that the affinity and specificity of peptides for GAGs can be tuned by adapting their amino acid sequence and the number of GAG-binding motifs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Gabor Toth, Domonkos Pal, Simon Sugar, Ilona Kovalszky, Katalin Dezso, Gitta Schlosser, Laszlo Drahos, Lilla Turiak
Summary: This study investigated the content and sulfation pattern of chondroitin sulfate (CS) and heparan sulfate (HS) in human hepatocellular carcinoma and cirrhotic liver tissues, considering the etiology of the diseases. Major differences were observed in the total abundance and sulfation pattern of CS and HS chains, providing important insights into the molecular mechanisms of chronic liver diseases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Clinical Neurology
Natalia Perez-Lopez, Carla Martin, Beatriz Garcia, Maria Pilar Solis-Hernandez, David Rodriguez, Ignacio Alcalde, Jesus Merayo, Ivan Fernandez-Vega, Luis M. Quiros
Summary: The saccharide chains of heparan sulfate play a crucial role in the pathogenesis of Alzheimer's disease, with differential transcription of biosynthetic genes observed in different brain regions and stages of AD pathology. The study revealed more alterations in gene transcription in moderate AD patients compared to mild cases, with fewer changes in genes related to early stages of synthesis in severe patients and an overexpression of late-stage genes. These alterations correlated with progressive brain atrophy, particularly in the cerebellum, and were consistent with immunohistochemical detection of certain heparan sulfate epitopes.
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ryuichi Mashima, Torayuki Okuyama, Mari Ohira
Summary: Heparan sulfate (HS) plays a crucial role in various biological functions such as neurology, skeletal development, immunology, and tumor metastasis. The biosynthesis of HS involves several steps including linkage, polymerization, and modification. HS degradation is predominantly mediated by lysosomes. Mucopolysaccharidosis is a genetic disorder associated with HS metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Maria C. Z. Meneghetti, Paula Deboni, Carlos M. V. Palomino, Luiz P. Braga, Renan P. Cavalheiro, Gustavo M. Viana, Edwin A. Yates, Helena B. Nader, Marcelo A. Lima
Summary: Heparan sulfate (HS), a polysaccharide found on the cell surface and extracellular matrix, is synthesized in the Golgi apparatus through a non-template driven process. Changes in the localization of HS-modifying enzymes in the Golgi correlate with changes in the structure of HS, rather than protein expression levels. Understanding the dynamics of HS-modifying enzymes through vesicular trafficking in the ER-Golgi pathway is crucial for a comprehensive understanding of HS biosynthesis.
CARBOHYDRATE POLYMERS
(2021)
Review
Biochemistry & Molecular Biology
Anastasia V. Strokotova, Elvira V. Grigorieva
Summary: Glucocorticoids are commonly used to treat various diseases but can cause side effects. Recent studies suggest that the toxic effects of glucocorticoids on key extracellular components in the brain may be an underlying mechanism for these side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Brigida Bochicchio, Giselle C. Yeo, Pearl Lee, Deniz Emul, Antonietta Pepe, Antonio Laezza, Nicola Ciarfaglia, Daniela Quaglino, Anthony S. Weiss
Summary: This study identifies novel cell-adhesive sites in the tropoelastin N-terminal region between domains 12 and 16, which interact with alpha V and alpha 5 beta 1 integrin receptors to facilitate cell attachment and spreading. The findings suggest that domains 12-16 of tropoelastin direct initial cell attachment through cell-surface heparan sulfate glycosaminoglycans, followed by integrin-promoted attachment and spreading, advancing our understanding of elastin matrix biology.
Article
Biochemical Research Methods
Margaret Downs, Manveen K. Sethi, Rekha Raghunathan, Matthew D. Layne, Joseph Zaia
Summary: In this study, the matrisome proteins in Parkinson's disease (PD) were analyzed and significant differences were found in the expression of collagen proteins compared to control samples. Histological analysis supported these findings and showed similar trends in collagen abundance. The hydroxyprolination of collagen was less variable in PD compared to controls, and glycoproteomic changes were observed. Network topology-based analysis revealed the involvement of angiogenesis-related proteins in PD. Further studies with a larger cohort are needed to validate these results.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Gustavo J. Cavallero, Joseph Zaia
Summary: The combination of HILIC and cIM-MS proved to be an effective method for separating HS oligosaccharide isomers, including positional isomers and sulfate positions. This approach provides high-quality data for the discovery of new structural motifs for HS binding proteins and for targeted quality control analysis of commercial HS products.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Deborah Chang, Joshua Klein, William E. Hackett, Mary Rachel Nalehua, Xiu-Feng Wan, Joseph Zaia
Summary: Glycosylation plays a critical role in the function and evolution of influenza A virus hemagglutinin (HA). The use of data-independent acquisition mass spectrometry allows for quantitative measurement of glycosylation changes and comparison between different viral expression systems and variants.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Cell Biology
Marissa L. Maciej-Hulme, James Melrose, Brooke L. Farrugia
Summary: Chondroitin sulfate (CS) is a glycosaminoglycan attached to the proteins of various proteoglycans. This review explores the role of CS and associated proteoglycans in arthritis and Duchenne muscular dystrophy (DMD), highlighting their potential as biomarkers and therapeutic targets for these pathologies.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biochemical Research Methods
Joseph Zaia
Summary: The 2022 Nobel Prize in Chemistry was awarded for the development of biorthogonal chemical ligation reactions, also known as click chemistry, in biomedicine. This concept has significantly advanced sensing and diagnosis, chemical biology, materials chemistry, and drug discovery and delivery. In the field of proteomics, the incorporation of click tags into proteins has revolutionized the selective enrichment of protein complexes and the study of protein networks, as well as the enrichment of protein post-translational modifications. This feature article summarizes the impact of biorthogonal click chemistry on proteomics.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Marissa L. Maciej-Hulme, Anaelle C. N. Leprince, Andre Lavin, Scott E. Guimond, Jeremy E. Turnbull, Julien Pelletier, Edwin A. Yates, Andrew K. Powell, Mark A. Skidmore
Summary: The fine structure of heparan sulfate plays a crucial role in cell signaling processes, infection, and other biochemical processes. An ultra-high sensitivity method utilizing reverse-phase HPLC and laser-induced fluorescence detection is described, which greatly improves the detection of HS disaccharides by six orders of magnitude. This method allows for the analysis of HS composition in minute tissue samples.
ANALYTICAL METHODS
(2023)
Article
Biochemical Research Methods
Tin Long Wong, Brian P. Mooney, Gustavo J. Cavallero, Minhui Guan, Lei Li, Joseph Zaia, Xiu-Feng Wan
Summary: In this study, a glycoproteomic approach using the timsTOF Pro mass spectrometer was developed to analyze site-specific glycosylation in influenza glycoproteins. The method proved to be effective in quantifying glycopeptides and identifying low-abundance glycopeptides. The results showed variations in glycan types and quantities between different influenza viruses.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jenna R. James, Johnathan Curd, Jennifer C. Ashworth, Mays Abuhantash, Martin Grundy, Claire H. Seedhouse, Kenton P. Arkill, Amanda J. Wright, Catherine L. R. Merry, Alexander Thompson
Summary: A 3D model of the bone marrow niche has been developed using a synthetic peptide hydrogel (SAPH) to screen FDA-approved drugs for acute myeloid leukemia (AML). The optimized stiffness of SAPH affects AML cell proliferation and Salinomycin shows efficacy in AML patient samples. Vidofludimus is not sensitive to AML cells in the hydrogel models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Marjolein Garsen, Baranca Buijsers, Marloes Sol, Lena Gockeln, Ramon Sonneveld, Toin H. van Kuppevelt, Mark de Graaf, Jacob van den Born, Jan A. A. M. Kamps, Daniel H. van Raalte, Rutger W. van der Meer, Hildo J. Lamb, Jan-Luuk Hillebrands, Ton J. Rabelink, Marissa L. Maciej-Hulme, Guido Krenning, Tom Nijenhuis, Johan van der Vlag
Summary: PPAR y agonists can ameliorate proteinuria by inhibiting the expression of glomerular HPSE, providing a potential mechanism for the renoprotective effects of these drugs.
Article
Biochemistry & Molecular Biology
Marissa L. L. Maciej-Hulme, Jasper J. J. Van Gemst, Patience Sanderson, Angelique L. W. M. M. Rops, Jo H. H. Berden, Bart Smeets, I. Jonathan Amster, Ton J. J. Rabelink, Johan van der Vlag
Summary: Proliferative forms of glomerulonephritis are characterized by leukocyte influx, albuminuria, and loss of kidney function. The glycocalyx, comprised of heparan sulfate, plays a crucial role in glomerular inflammation. Exogenous glomerular glycocalyx and low-molecular-weight heparin reduce proteinuria and granulocyte/macrophage influx in experimental glomerulonephritis, as well as prohibit granulocyte adhesion to glomerular endothelial cells. The development of HS-based therapeutics for inflammatory glomerular diseases is justified by these findings.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Editorial Material
Biochemical Research Methods
Shoumita Dasgupta, Joseph Zaia
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Editorial Material
Biochemical Research Methods
Joseph Zaia
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Baranca Buijsers, Marissa Maciej-Hulme, Maaike Jacobs, Marinka Bakker-van Bebber, Mark de Graaf, Rustem Salmenov, Naomi Parr, Ton J. J. Rabelink, Tom Nijenhuis, Johan van der Vlag
Summary: The study explored the potential protective effects of unstimulated HSglx, LPS-stimulated HSglx, fucoidan, and sulodexide in experimental glomerulonephritis. The findings revealed that these compounds could attenuate proteinuria and modulate inflammatory responses in glomerular diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Juan Wei, Dimitris Papanastasiou, Mariangela Kosmopoulou, Athanasios Smyrnakis, Pengyu Hong, Nafisa Tursumamat, Joshua A. A. Klein, Chaoshuang Xia, Yang Tang, Joseph Zaia, Catherine E. E. Costello, Cheng Lin
Summary: Comprehensive de novo glycan sequencing is challenging due to the diversity and complexity of glycans. This study demonstrates the effectiveness of electron excitation dissociation (EED) mass spectrometry for glycan analysis, showing high sensitivity, fragmentation efficiency, and analysis speed. A novel EED MS2-guided MS3 approach is developed, allowing accurate determination of glycan topology and linkage configurations.
Article
Biochemistry & Molecular Biology
Guinevere S. M. Lageveen-Kammeijer, Erdmann Rapp, Deborah Chang, Pauline M. Rudd, Carsten Kettner, Joseph Zaia
Summary: The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative to standardize the reporting of glycoanalytical methods and to assess their reproducibility. They have published several reporting guidelines that describe the information that should be provided for glycoanalytical methods, and have now presented the first version of reporting guidelines for glyco(proteo)mics analysis by capillary electrophoresis (CE).