G protein gamma 7 suppresses progression of lung adenocarcinoma by inhibiting E2F transcription factor 1

G protein gamma 7 (GNG7) has been found to be aberrantly expressed in some kinds of malignant tumors. In this study, we mainly discuss the antitumor role of it in lung adenocarcinoma (LUAD) cells. Protein levels of GNG7 in LUAD tissues were measured by western blot and immunohistochemical analysis. Cell proliferation, invasion and migration were detected by CCK-8 assay, 5-ethynyl-2 '-deoxyuridine (EdU), and Transwell assay. In our study, GNG7 was down-regulated in LUAD, which significantly correlated with survival of LUAD patients. Functional experiments revealed that GNG7 significantly inhibited LUAD cell proliferation, migration, and invasion in vitro and E2F1 overexpression reversed these properties. GNG7 suppressed xenograft tumorigenesis in nude mice models in vivo. In conclusion, GNG7 functions as a tumor suppressor in LUAD cells through inhibiting E2F1. (c) 2021 Published by Elsevier B.V.

Automated summary

The G protein gamma 7 (GNG7) has been found to be aberrantly expressed in certain malignant tumors, with a study focusing on its antitumor role in lung adenocarcinoma (LUAD) cells. Down-regulation of GNG7 in LUAD tissues was found to correlate with patient survival. Functional experiments demonstrated that GNG7 acts as a tumor suppressor in LUAD cells by inhibiting E2F1 and suppressing cell proliferation, migration, and invasion.