Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 183, Issue -, Pages 1715-1722Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.05.120
Keywords
Astragalus polysaccharide; Myelosuppression; Protective effect; Hematopoietic stem cell; FOS
Funding
- HKSAR Innovation and Technology Fund (ITF), Tier 3 [ITS/311/09]
- General Research Fund [12100615, 22100014, 12100818]
- UGC Research Matching Grant Scheme [2019-1-10, 2019-1-14, 2019-2-06]
- Health Medical Research Fund [11122531, 14150521, 17182681]
- National Natural Science Foundation of China [81473341]
- Science and Technology Project of Shenzhen [JCYJ20160531193812867]
- Key-Area Research and Development Program of Guangdong Province [2020B1111110007]
- Hong Kong Baptist University [RC-Start-up Grants] [MPCF-001-2016/2017, RC-IRMS/14-15/06, FRG2/17-18/060, FRG2/16-17/002]
- Vincent & Lily Woo Foundation
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Radix Astragali polysaccharide (RAP) directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression, demonstrating a significant protective effect in hematopoiesis.
Radix Astragali polysaccharide RAP has been reported to play a crucial role in hematopoiesis without a clear mechanism. In this study, RAP's effects to enhance the recovery of cyclophosphamide (Cy)-suppressed bone marrow and blood cells is confirmed in vivo first. Confocal micrographs demonstrated the interesting direct binding of FITC-RAP to hematopoietic stem cells (HSC) in bone marrow. RAP protects both mice and human HSC in terms of cell morphology, proliferation, and apoptosis. RNA-sequencing and shRNA approaches revealed FOS to be a key regulator in RAP's protection. These evidences provide an unreported mechanism that RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression. (c) 2021 Elsevier B.V. All rights reserved.
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