Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties

In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated. Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography. Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of -> 2)-alpha-D-Manp-(1 ->, -> 3)-alpha-D-Manp-(1 -> and with branches containing alpha-D-Manp-(1 ->, EPS-2 had a backbone composed of -> 6)-beta-D-Glcp-(1 ->, and with branches containing -> 2)-alpha-L-Fucp-(1 ->, -> 3)alpha-D-Glcp-(1 ->, -> 2)-alpha-D-Glcp-(1 ->, beta-D-Glcp-(1 ->, and alpha-D-Glcp-(1 ->. Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity. Also, it has a good ability to anti-biofilms. Overall, the analysis of all data showed EPS from P. acidilactici MT41-11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.

Automated summary

This study investigated the chemical structure and bioactive properties of EPS isolated from Pediococcus acidilactici MT41-11 from camel milk. The EPS showed multiple bioactive properties, such as stimulating Lactobacillus growth, high DPPH free radical scavenging activity, and good anti-biofilm ability. Overall, the EPS has potential applications as an antioxidant, anti-biofilm agent, and a prebiotic for the health food or medicine industry.