☆ 4.7 Article

Elevated linc00936 or silenced microRNA-425-3p inhibits immune escape of gastric cancer cells via elevation of ZC3H12A

INTERNATIONAL IMMUNOPHARMACOLOGY (2021)

Journal

INTERNATIONAL IMMUNOPHARMACOLOGY

Volume 95, Issue -, Pages -

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ELSEVIER

DOI: 10.1016/j.intimp.2021.107559

Keywords

Gastric cancer; Long intergenic noncoding RNA00936; MicroRNA-425-3p; Immune escape; Cytotoxicity; Migration; Apoptosis; Monocyte chemotactic protein-induced protein 1

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Abstract

This study identified the important role of the linc00936/miR-425-3p/ZC3H12A axis in regulating immune escape of GC cells. Up-regulating linc00936 or silencing miR-425-3p can inhibit immune escape of GC cells and enhance the cytotoxicity of CIK cells.

Objective: Gastric cancer (GC) is a malignant tumor originated from gastric mucosa. Without effective therapy, this study was to investigate the mechanism of long intergenic noncoding RNA 00936 (linc00936)/microRNA425-3p (miR-425-3p)/monocyte chemotactic protein-induced protein 1 (ZC3H12A) axis mediating immune escape of GC cells. Methods: Peripheral blood samples, GC tissues and adjacent tissues were collected. The levels of CD3+, CD4+, and CD8+ in peripheral blood were detected. The expression levels of linc00936, miR-425-3p and ZC3H12A in GC tissues and cells were detected. The correlation between the expression of linc00936 in the tissues and the levels of CD3+, CD4+ and CD8+ in the peripheral blood of GC patients was analyzed. Cytokine-induced killer (CIK) cells were induced, and co-incubated with GC cells. BGC-823 and MKN-45 cells were screened and transfected with linc00936- or miR-425-3p-related oligonucleotides to figure out their roles in immune escape, migration, apoptosis and the cytotoxicity of CIK cells in GC cells. Results: Elevated miR-425-3p and reduced linc00936, and ZC3H12A expression levels were found in GC tissues and cells. Linc00936 expression was positively correlated with CD3+ and CD4+, and negatively correlated with CD8+ in peripheral blood of patients with GC. Up-regulating linc00936 or down-regulating miR-425-3p inhibited immune escape, migration, promoted apoptosis of GC cells, as well induced CIK cell cytotoxicity to GC cells. Down-regulated linc00936 or elevated miR-425-3p facilitated immune escape, migration, depressed apoptosis of GC cells, and reduced the cytotoxicity of CIK cells to GC cells. Conclusion: The study concludes that up-regulated linc00936 or silenced miR-425-3p inhibits immune escape of GC cells via elevation of ZC3H12A.

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Dysfunction of the ST7-AS1/miR-301b-3p/BTG1 ceRNA network promotes immune escape of triple-negative breast cancer

Yong Li, Wenge Xin, Fang Liu, Fengjuan Li, Chengmin Yang, Changmin Liu, Jiaxin Liu

Summary: This study explores the role of a newly constructed lncRNA-miRNA-mRNA ceRNA network in immune escape in triple-negative breast cancer (TNBC). The ceRNA regulatory network ST7-AS1/miR-301b-3p/BTG1, related to the prognosis of TNBC patients, was identified. In vivo experiments confirmed down-regulated expression of ST7-AS1 and BTG1, and up-regulated expression of miR-301b-3p in orthotopic transplanted tumor tissues of mice. It was found that ST7-AS1 might promote BTG1 expression by competitively binding to miR-301b-3p, thereby restricting immune escape in TNBC.

INTERNATIONAL IMMUNOPHARMACOLOGY (2023)