Article
Microbiology
Yuwei Zhang, Stephen L. W. On
Summary: Yersinia enterocolitica and Y. pseudotuberculosis are important causes of enteric illness worldwide. The use of cold enrichment methods for their detection is time-consuming, and there is limited evidence to support the claim that key pathogenicity markers are lost at higher temperatures. A study found that an enrichment step at 37 degrees C did not result in the loss of important markers, suggesting that this method could be used for more rapid detection of these pathogens.
Editorial Material
Hematology
Lee M. Greenberger, Gwen L. Nichols
Summary: In this issue of Blood, Mittelman et al(1) and Pagano et al(2) demonstrate that patients with hematologic malignancies who have been fully or partially vaccinated are at a higher risk of poor clinical outcomes after COVID-19 infection compared to matched controls or the general population.
Article
Cell Biology
Teodora Oltean, Lorena Itati Ibanez, Tatyana Divert, Tine Ysenbaert, Hannelore Van Eeckhoutte, Vera Goossens, Michael Schotsaert, Ken Bracke, Bert Schepens, Jonathan Maelfait, Nozomi Takahashi, Xavier Saelens, Peter Vandenabeele
Summary: RIPK3 partially protects against disease caused by influenza A virus infection in mice. Active vaccination with M2e viral antigen provides protection against IAV, but this protection is lost in Ripk3(-/-) mice.
CELL DEATH & DISEASE
(2022)
Letter
Medical Laboratory Technology
Alexandra R. Rapp, John O. Ogunbileje, Diane Djouodo-Nemzou, Anthony O. Okorodudu
Summary: Individuals vaccinated for COVID19 should have IgG antibodies in response to the specific antigen targeted in vaccine development, often the spike protein. Developing testing algorithms for anti-spike and anti-nucleocapsid antibodies can help differentiate infection from vaccination. Clear communication about specific SARS-CoV-2 antibodies in antibody tests is crucial to avoid confusion in interpreting serologic testing.
CLINICA CHIMICA ACTA
(2021)
Review
Immunology
Cinzia Milito, Valentina Soccodato, Giulia Collalti, Alison Lanciarotta, Ilaria Bertozzi, Marcello Rattazzi, Riccardo Scarpa, Francesco Cinetto
Summary: PADs, the most common PIDs, can be classified into different types, and vaccines play important roles in treatment, diagnosis, and prevention, although they may be unsafe or less effective in some cases. EMA-approved COVID-19 vaccines are recommended for PAD patients, but specific data on safety and efficacy are lacking.
Review
Biotechnology & Applied Microbiology
Paula Matos de Brito, Andreia Saruga, Miguel Cardoso, Joao Goncalves
Summary: Antibodies are essential components of the adaptive immune system with rapidly growing therapeutic applications. Various platforms in higher eukaryotic cells have been developed to overcome limitations and replicate antibody diversification mechanisms. The latest strategies aim to mimic both naive and affinity-maturated antibody repertoires.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Immunology
Nicole L. Messina, Mariana G. Sperotto, Marco A. M. Puga, Patricia V. da Silva, Roberto D. de Oliveira, Cecilia L. Moore, Laure F. Pittet, Tenaya Jamieson, Margareth Dalcolmo, Glauce dos Santos, Bruno Jardim, Marcus V. G. Lacerda, Nigel Curtis, Julio Croda, BRACE Trial Consortium Grp
Summary: This study compared antibody responses to CoronaVac (Sinovac) and ChAdOx1-S (AstraZeneca-Oxford) vaccines in 874 healthcare workers in Brazil. The results showed that ChAdOx1-S induced a stronger anti-spike IgG response than CoronaVac. Recent BCG vaccination did not affect the antibody responses to either vaccine.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Keerthana Chithanathan, Monika Jurgenson, Mithu Guha, Ling Yan, Tamara Zarkovskaja, Martin Pook, Nathaniel Magilnick, Mark P. Boldin, Ana Rebane, Li Tian, Alexander Zharkovsky
Summary: This study investigated the effect of miR-146b deficiency on LPS-induced neuroinflammation. The results showed that miR-146b deficiency attenuates LPS-induced neuroinflammation, as evidenced by reduced sickness behavior, decreased inflammatory status of microglia, and loss of morphological signs of microglial activation. The upregulation of miR-146a in Mir146b-/- mice was found to be caused by overexpression of the miR-146a transcription inducer Irf7.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Medicine, General & Internal
J-S Yen, I-K Wang, T-H Yen
Summary: Uremia affects all parts of the immune system, making dialysis patients more susceptible to COVID-19 infection. Different factors such as vaccine types, doses and individual characteristics can lead to varying response rates in dialysis patients. Vaccination against COVID-19 in this population is crucial for global public health.
QJM-AN INTERNATIONAL JOURNAL OF MEDICINE
(2021)
Article
Immunology
Alok R. Amraotkar, Adrienne M. Bushau-Sprinkle, Rachel J. Keith, Krystal T. Hamorsky, Kenneth E. Palmer, Hong Gao, Shesh N. Rai, Aruni Bhatnagar
Summary: The presence of chronic health conditions, especially chronic kidney disease and autoimmune disease, increases the likelihood of seronegative status among individuals who are fully vaccinated to SARS-CoV-2. The absence of any chronic health conditions is protective and increases the likelihood of a positive serological response. The risk of seronegative status increases with an increase in the number of comorbidities and the use of CHC-related medications.
Article
Immunology
Kristel Ramirez Valdez, Benjamin Nzau, Daniel Dorey-Robinson, Michael Jarman, James Nyagwange, John C. Schwartz, Graham Freimanis, Angela W. Steyn, George M. Warimwe, Liam J. Morrison, William Mwangi, Bryan Charleston, Marie Bonnet-Di Placido, John A. Hammond
Summary: Studying the antibody response to infection or vaccination is crucial for developing more effective vaccines and treatments. The use of high-throughput antibody sequencing technologies and immunoinformatic tools now allows for fast and detailed analysis of antibody repertoires in any species. This article presents a suite of customizable methods for studying the cattle antibody response, including flow cytometry, single cell sorting, amplification, and sequencing. These methods, when combined with annotation tools, provide a powerful toolkit for studying antibody responses with high resolution and precision.
Article
Immunology
Cas LeMaster, Eric S. Geanes, Elizabeth R. Fraley, Rangaraj Selvarangan, Todd Bradley
Summary: The Omicron variant of SARS-CoV-2 has shown immune escape, causing infections even among vaccinated individuals or those with prior COVID-19. This study found that the Pfizer COVID-19 mRNA vaccine resulted in decreased binding and neutralizing antibody responses to the Omicron variant. However, individuals who had recovered from COVID-19 before vaccination showed higher levels and avidity of antibodies against the Omicron variant compared to those who were only vaccinated. This suggests that prior COVID-19 infection can generate a stronger immune response to the Omicron variant, and repeated exposure through infection or vaccination may be necessary to improve immunity to emerging variants.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Helen R. Wagstaffe, Elizabeth A. Clutterbuck, Viki Bockstal, Jeroen N. Stoop, Kerstin Luhn, Macaya Douoguih, Georgi Shukarev, Matthew D. Snape, Andrew J. Pollard, Eleanor M. Riley, Martin R. Goodier
Summary: This study highlights the importance of antibody concentration and NK cell differentiation status in promoting Fc-mediated NK cell activation after Ebola virus vaccination, suggesting a potential role for antibody-mediated NK cell activation in vaccine-induced immune responses.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Anette Tarp Hansen, Anna Soderstrom, Charlotte Svaerke Jorgensen, Carsten Schade Larsen, Mikkel Steen Petersen, Jens Magnus Bernth Jensen
Summary: The study found that only 17% of adult patients with suspected primary immunodeficiency diseases fully complied with the AAAAI guidelines for diagnostic vaccination, indicating the guidelines are difficult to follow. Furthermore, the arbitrary choices of serotype-specific antibodies for interpretation heavily influenced the data interpretation, revealing an inherent flaw in the guidelines. The proposed revised protocol of diagnostic vaccination, subsequent antibody measurements, and data interpretation using Z-scores provided more robust clinical results compared to interpretation according to the AAAAI guidelines.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Dominique J. Barbeau, Judith M. Martin, Emily Carney, Emily Dougherty, Joshua D. Doyle, Terence S. Dermody, Alejandro Hoberman, John Williams, Marian G. Michaels, John F. Alcorn, W. Paul Duprex, Anita K. McElroy
Summary: This study observed the immune responses elicited by the SARS-CoV-2 vaccines BNT162b2, mRNA-1273, and Ad26.COV2.S. The results showed that mRNA-based vaccines elicited higher magnitude humoral responses, with mRNA-1273 inducing the most durable response, but all humoral responses decreased over time. Neutralizing antibodies against the Delta variant were lower for all three vaccines. The T cell response initially induced by mRNA-1273 was the most significant, but declined after six months. Therefore, declining immunity over time emphasizes the importance of booster doses.
Article
Fisheries
Ilhan Aydin, Rafet Cagri Ozturk, Ercan Kucuk, Hamza Polat, Ilhan Altinok
Summary: This study compared the hatchery and growth performance of diploid and triploid turbot at 16 degrees C and 21 degrees C. Different water temperatures during rearing had significant effects on the weight and length of turbot, with triploids showing differences in growth performance.
Article
Fisheries
Mustafa Ture, Ilhan Altinok, Ayse Cebeci, Nihal Caliskan
AQUACULTURE RESEARCH
(2022)
Article
Ecology
Rafet Cagri Ozturk, Ali Muzaffer Feyzioglu, Ilhan Altinok
Summary: This study describes the microbial community compositions of coastal surface waters of the Western Antarctic Peninsula (WAP), identifying 157 bacteria species and two Archaea taxa. The prevalence of Proteobacteria and Bacteroidetes was observed among the bacterial phyla. Pseudoalteromonadaceae was the dominant family, representing almost 94% of the generated sequences.
Article
Fisheries
lhan Aydin, Rafet Cagri Oztuerk, Yahya Terzi, Ercan Kuecuek, Hamza Polat, Ilhan Altinok
Summary: This study investigated skeletal development and malformations in diploid and cold shock-induced triploid turbot during the early life stages. The results showed no difference in skeletal development between ploidy groups, with vertebral elements mostly remaining cartilaginous until 15 days post-hatch. Cold shock-induced triploid turbot had no disadvantage in terms of development and remained a feasible alternative for the turbot aquaculture industry. The most frequently encountered malformation was of the neural arch or spine, with significantly higher frequencies in diploid turbot in the caudal region.
Article
Fisheries
Ilhan Altinok, Rafet Cagri Ozturk, Mustafa Ture
Summary: The whole genome sequence of Lactococcus garvieae Lg-per, originally isolated from rainbow trout in Turkey, was analyzed. The study revealed that Lg-per was actually Lactococcus petauri, not L. garvieae as previously believed. The 16S rRNA-based PCR detection method was found to be inadequate for identifying Lactococcus strains. This is the first report of L. petauri in Turkey and highlights the importance of using whole genome sequencing for analyzing L. garvieae isolates in other countries.
JOURNAL OF FISH DISEASES
(2022)
Article
Agriculture, Multidisciplinary
I Yandi, R. C. Ozturk, Y. Terzi, S. Kayis, I Altinok
Summary: This study evaluated the effects of including black soldier fly prepupae in rainbow trout fingerling diets. The results showed that replacing 50% of fish meal with black soldier fly meal had no adverse effects on growth performance, liver characteristics, feed utilization, or fillet quality. Therefore, black soldier fly meal can safely be used in rainbow trout fingerling diets.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Review
Biodiversity Conservation
Myriam Heuertz, Silvia B. Carvalho, Juan Galindo, Baruch Rinkevich, Piotr Robakowski, Tsipe Aavik, Ilhan Altinok, Julia M. I. Barth, Helena Cotrim, Roos Goessen, Santiago C. Gonzalez-Martinez, Tine Grebenc, Sean Hoban, Alexander Kopatz, Barry J. McMahon, Ilga Porth, Joost A. M. Raeymaekers, Sabrina Traeger, Alejandro Valdecantos, Adriana Vella, Cristiano Vernesi, Pauline Garnier-Gere
Summary: Conserving biodiversity at genetic and community levels is crucial for the provision of ecosystem services. However, the application of genomics in biodiversity conservation and ecosystem service management is currently limited, primarily focusing on flagship species. To bridge this gap, a framework is proposed to guide genomics applications in management, including population supplementation, assisted migration, delimitation of conservation areas, and solving conservation trade-offs. Effective implementation of genomics-informed management actions requires collaboration between scientists and ecosystem service managers along with standardized workflows and training for managers.
BIOLOGICAL CONSERVATION
(2023)
Article
Fisheries
Rafet Cagri Ozturk, Ilhan Yandi, Yahya Terzi, Ilhan Altinok
Summary: This study investigated the effects of direct black soldier fly (BSF) feeding on the growth, health, and filet quality of rainbow trout. It was found that feeding dried BSF led to a reduction in growth, decreased feed intake, and constipation in the fish. The amino acid content of BSF prepupae was higher than that of commercial diet, and some fatty acid contents were similar to or higher than commercial diet. Feeding dried BSF once a day resulted in improvements in certain fatty acid levels in the fish.
TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
I. Yandi, R. C. Ozturk, M. Kocabas, I. Z. Kurtoglu, I. Altinok
Summary: This study investigated the effect of different organic waste substrates on the nutritional content of black soldier fly prepupae (BSFP). It found that BSFP reared on fruit and vegetable waste had lower protein and lipid content, but higher carbohydrate content compared to those reared on chicken waste meal and a mixture of the two. Additionally, BSFP reared on the mixture had higher levels of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids. These findings suggest that the use of certain organic waste substrates can enhance the nutritional value of BSFP and their potential as a sustainable protein source in aquafeeds.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Article
Fisheries
Sarker Mohammed Ibrahim Khalil, Chiara Bulfon, Marco Galeotti, Pier Luigi Acutis, Ilhan Altinok, Charalampos Kotzamanidis, Ana Isabel Vela, Lucio Fariano, Marino Prearo, Silvia Colussi, Donatella Volpatti
Summary: This study provides preliminary knowledge on the immunological relationship between rainbow trout and L. garvieae, suggesting that specific immune parameters could serve as reliable indicators of the fish's innate ability to fight infection.
JOURNAL OF FISH DISEASES
(2023)
Article
Microbiology
Nadia Stoppani, Silvia Colussi, Paolo Pastorino, Marino Prearo, Simona Sciuto, Ilhan Altinok, Rafet Cagri Ozturk, Mustafa Ture, Ana Isabel Vela, Maria del Mar Blanco, Charalampos Kotzamanidis, Konstantina Bitchava, Andigoni Malousi, Lucio Fariano, Donatella Volpatti, Pier Luigi Acutis, Jose Francisco Fernandez-Garayzabal
Summary: The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate L. garvieae from L. petauri for accurate discrimination between these two species. Seven SNPs were identified that separate L. garvieae from L. petauri based on the ITS region sequence. The 16S-23S rRNA ITS region can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak.
Review
Fisheries
Raja Aadil Hussain Bhat, Ilhan Altinok
Summary: Antimicrobial resistance in aquaculture poses a significant threat to public health and the environment. Understanding the mechanisms driving AMR and the patterns of antibiotic usage is crucial for developing risk management strategies. Alternative approaches can be adopted to prevent and control fish diseases without negatively impacting ecosystems and public health.
TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
(2023)
Article
Microbiology
Ayse Cebeci, Mustafa Ture, Melike Alemdag, Ilhan Altinok
Summary: In this study, a bacteriophage specific to Aeromonas hydrophila was isolated from domestic wastewater. The phage was found to be icosahedral with a diameter of 60 nm. Based on the analysis of its genome sequence, the phage belongs to the genus Lahexavirus and does not contain antimicrobial resistance or virulence genes. Overall, the Lahexavirus APT65 phage may have potential as a therapeutic agent to tackle antimicrobial resistance in aquaculture.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2023)
Article
Fisheries
Ana Isabel Vela, Maria del Mar Blanco, Silvia Colussi, Charalampos Kotzamanidis, Marino Prearo, Ilhan Altinok, Pier Luigi Acutis, Donatella Volpatti, Patricia Alba, Fabiola Feltrin, Angela Ianzano, Lucas Dominguez, Jose Francisco Fernandez-Garayzabal
Summary: Lactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. This study reveals that both Lactococcus garvieae and Lactococcus petauri are implicated in the etiology of lactococcosis, and that L. petauri plays a significant role in the epidemiology of this disease. Additionally, some biochemical differences between the two species may serve as phenotypic markers for their presumptive recognition.
