Article
Engineering, Environmental
Weicheng Zhou, Xiaoyu Li, Yuming Wang, Jinglong Wang, Jinli Zhang, Hui Wei, Chengrong Peng, Zhicong Wang, Genbao Li, Dunhai Li
Summary: Exposure to 2-MIB affects developmental parameters, locomotive behavior, oxidative stress, apoptosis, and transcriptome of zebrafish. However, it does not cause changes in mortality, hatchability, and malformation rate. Zebrafish larvae show increased body length in a dose-dependent manner, accompanied by alterations in GH/IGF and HPT axis genes. Additionally, 2-MIB induces increased swimming activity in zebrafish larvae.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Ping Ding, Chongdan Xiang, Xintong Li, Haibo Chen, Xiaoxia Shi, Xin Li, Chushan Huang, Yunjiang Yu, Jianying Qi, Adela Jing Li, Lijuan Zhang, Guocheng Hu
Summary: Microplastics (MPs) are pervasive environmental contaminants that induce neurotoxicity in various organisms. This study revealed that photoaged polystyrene (P-PS) caused more severe neurotoxic effects compared to virgin polystyrene (V-PS) in zebrafish. Exposure to P-PS altered antioxidant enzyme activity, neurotransmitter levels, and behavior in larval zebrafish. Furthermore, the expression of genes related to neurotransmission and oxidative stress was significantly affected by PS exposure. These findings provide new insights into the potential risks of MPs in the environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Sathisaran Umamaheswari, Sheela Priyadarshinee, Monojit Bhattacharjee, Krishna Kadirvelu, Mathan Ramesh
Summary: The study shows that polystyrene microplastics induce various toxic effects in zebrafish, including disrupting the antioxidant defense system, causing histopathological lesions, and altering gene expression patterns.
Article
Environmental Sciences
Ziyue Shi, Xuefang Liang, Yaqian Zhao, Wang Liu, Christopher J. Martyniuk
Summary: This study evaluated the potential neurotoxicity of three synthetic phenolic antioxidants (SPAs) on zebrafish larvae. The results showed that different SPAs had different effects on the behavior and gene expression of zebrafish, possibly through modulation of signaling pathways such as dopamine, serotonin, and GABA, leading to neurotoxicity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Qiong Zhang, Shukai Zheng, Xiaoling Shi, Congying Luo, Wenlong Huang, Henghui Lin, Jiajun Peng, Wei Tan, Kusheng Wu
Summary: As a substitute for PBDEs, TPhP is widely used in daily products and living surroundings, but information on its neurodevelopmental toxicity is lacking. This study investigated the effects of TPhP exposure on zebrafish neural development and the underlying molecular mechanisms. Results showed that TPhP exposure reduced survival rate, hatchability, heart rate, body length, and caused malformations in zebrafish. It also led to abnormal locomotor behaviors and impaired panic avoidance reflex. TPhP induced oxidative stress, activated mitochondrial signaling pathways, and affected apoptosis, resulting in visual impairment.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Marine & Freshwater Biology
Rui-ying Guo, Jing Xiang, Li -jun Wang, Er-chao Li, Ji-liang Zhang
Summary: In this study, the effects of tributyltin (TBT) on the fish circadian rhythm system were investigated using zebrafish. It was found that TBT exposure disrupted the locomotor activity rhythms of zebrafish and caused changes in the histological structure of the eyes and decreased melanin concentrations. Pathway analyses revealed that differentially expressed genes were enriched in metabolism pathways. Moreover, TBT exposure resulted in decreased melanin and disruption of retinoic acid, affecting the reception of phototransduction and interfering with the circadian rhythm in fish.
AQUATIC TOXICOLOGY
(2022)
Article
Environmental Sciences
Yumiao Zhou, Qiang Kong, Zhihao Lin, Jinyue Ma, Huanxin Zhang
Summary: The study reveals that B[a]P has an impact on the locomotor behavior of zebrafish, with different effects at different concentrations. Through RNA sequencing analysis, it was found that the affected genes are mainly involved in cardiomyocyte signaling pathways and locomotory behavior.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Biology
Viacheslav V. Krylov, Evgeny I. Izvekov, Vera V. Pavlova, Natalia A. Pankova, Elena A. Osipova
Summary: This review discusses the circadian behavior rhythms of zebrafish, focusing on factors influencing these rhythms including ontogeny, environmental factors, and the modulation of physiological state. The complex interactions among different influences are highlighted, emphasizing the importance of considering them in behavioral studies.
BIOLOGICAL REVIEWS
(2021)
Article
Environmental Sciences
Menglan Su, Rongkai Bao, Yaqing Wu, Bo Gao, Peng Xiao, Wenhua Li
Summary: The developmental toxicity and hepatotoxicity of diafenthiuron in zebrafish were investigated in this study. Results showed that diafenthiuron exposure significantly shortened the body lengths of zebrafish larvae and decreased superoxide dismutase activity. It also downregulated the spatiotemporal expression of pituitary development marker genes and liver-specific marker genes. These findings provide evidence of the detrimental effects of diafenthiuron on aquatic organisms and are important for environmental risk assessment.
Article
Biochemistry & Molecular Biology
Luis Felix, Carolina Lobato-Freitas, Sandra M. Monteiro, Carlos Venancio
Summary: The study found that 24-epibrassinolide (24-EPI) can mitigate the teratogenic effects of caffeine during early zebrafish development, particularly reducing tail curvature. However, caffeine exposure resulted in locomotor deficits and disrupted anxiety responses. Coexposure to caffeine and the highest 24-EPI concentrations showed less pronounced behavioral deficits.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Environmental Sciences
Zhiyuan Meng, Zhichao Wang, Xiaojun Chen, Yueyi Song, Miaomiao Teng, Tianle Fan, Yang Zheng, Jiajia Cui, Wangjin Xu
Summary: Flubendiamide has bioaccumulation and toxic effects on zebrafish, causing liver damage and apoptosis, with further experiments aiding in the assessment of its safety to aquatic organisms.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Javad Seyedi, Mohammad Behzadi Tayemeh, Milad Esmaeilbeigi, Hamid Salari Joo, Elnaz Keshtkar Langeroudi, Ashkan Banan, Seyed Ali Johari, Mohammad Javad Jami
Summary: This research evaluated the antioxidant capability of quercetin against silver nanoparticles toxicity towards the lipid contents of ovarian, nervous, and hepatic systems as well as skeletal muscles. The findings suggest that unsaturated acyl chains, particularly HUFAs, in the liver tissue and oocyte cell are highly susceptible to peroxidation or degeneration by AgNPs. The alteration in HUFAs and PUFAs of the liver and oocyte could impact on maternal and offspring health and consequently alter long-term population dynamics of aquatic animals.
ENVIRONMENTAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Xiao Y. Li, Ying J. Qin, Yue Wang, Tao Huang, Yuan H. Zhao, Xiao H. Wang, Christopher J. Martyniuk, Bing Yan
Summary: This study evaluated the potential toxicity of four commonly used strobilurin fungicides on larval zebrafish, finding that pyraclostrobin and trifloxystrobin induced mitochondrial dysfunction and hypoactive responses in zebrafish. However, azoxystrobin and kresoxim-methyl had no significant effects on mitochondrial function or locomotion in zebrafish. The findings suggest a potential for adverse effects in aquatic organisms due to widespread detection of strobilurins in aquatic environments.
Article
Environmental Sciences
Giuseppe De Marco, Gea Oliveri Conti, Alessia Giannetto, Tiziana Cappello, Mariachiara Galati, Carmelo Iaria, Eloise Pulvirenti, Fabiano Capparucci, Angela Mauceri, Margherita Ferrante, Maria Maisano
Summary: In recent years, there has been increasing concern regarding the presence and accumulation of plastic debris in the environment, as well as the impact of microplastics (MPs) on biota. This study conducted a fish embryo toxicity test on zebrafish exposed to polystyrene MPs, revealing that the MPs had a moderate delay on hatching and caused deformities and compromised visual structure in larvae development. Additionally, increased gene transcription involved in oxidative stress and cellular detoxification were observed in the MPs-exposed zebrafish larvae.
ENVIRONMENTAL RESEARCH
(2022)
Article
Marine & Freshwater Biology
Teresa Capriello, Sandra M. Monteiro, Luis M. Felix, Aldo Donizetti, Vincenza Aliperti, Ida Ferrandino
Summary: The study revealed that aluminium significantly impacted the development and cell death of zebrafish, while also altering the oxidative state of larvae. At the highest concentration, aluminium toxicity led to decreased activity levels of certain antioxidant enzymes.
AQUATIC TOXICOLOGY
(2021)
Article
Environmental Sciences
Teresa Capriello, Gianluca Di Meglio, Anna De Maio, Rosaria Scudiero, Anna Rita Bianchi, Marco Trifuoggi, Maria Toscanesi, Antonella Giarra, Ida Ferrandino
Summary: Aluminium is a non-essential metal to human metabolism, but it is considered an etiological factor in neurodegenerative diseases. Research using zebrafish as an animal model suggests that aluminium can affect the morphology of brain tissue and contribute to the dysregulation of genes related to Parkinson's disease.
Article
Environmental Sciences
Luis Felix, Paulo Carreira, Francisco Peixoto
Summary: This study investigates the biological effects of naturally weathered microplastics (MPs) on zebrafish, revealing anxiety-like behavior and mitochondrial dysfunction caused by the MPs. It also emphasizes the potential cumulative impact of these particles on aquatic ecosystems.
Review
Fisheries
Luis Felix, Raquel Vieira, Sandra M. Monteiro, Carlos Venancio
Summary: The use of anaesthetic agents, including natural substances such as clove oil, as alternatives to the commonly used synthetic anaesthetic MS-222, has been investigated for fish anaesthesia. A meta-analysis of 30 English reports found that 10 different monoterpenes showed high effectiveness in inducing anaesthesia and recovery in fish. The findings also revealed an inverse linear association between anaesthesia induction and recovery time and water bath temperature.
FISH AND FISHERIES
(2023)
Article
Biochemistry & Molecular Biology
Francisco Pereira, Adriana Pereira, Sandra M. Monteiro, Carlos Venancio, Luis Felix
Summary: This study aimed to evaluate the antioxidant effects of a natural plant hormone, 24-epibrassinolide, against the teratogenic effects of nicotine on zebrafish embryos. The results showed that 24-epibrassinolide reduced nicotine-induced deformities in zebrafish embryos, while improving oxidative stress and metabolic changes. It also alleviated nicotine-induced cognitive dysfunction.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anna Rita Bianchi, Alessandra La Pietra, Valeria Guerretti, Anna De Maio, Teresa Capriello, Ida Ferrandino
Summary: By studying the brains of adult zebrafish exposed to aluminum, we found hyperactivation of poly(ADPribosyl)ation and changes in the synthesis and degradation of poly(ADP-ribose). The highest activity levels of poly(ADP-ribose) synthesis and degradation were observed after 10 and 15 days of exposure. We hypothesize that the activation of poly(ADP-ribose) polymerase (PARP) is related to aluminum-induced DNA damage, while the activation of poly(ADPR) glycohydrolase (PARG) is necessary to avoid the accumulation of poly(ADP-ribose).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Environmental Sciences
Germano A. B. Lanzarin, Luis M. Felix, Antonio Fontainhas-Fernandes, Sandra Mariza Monteiro, Carlos Venancio
Summary: A critical review was conducted to investigate the toxicity of glyphosate and glyphosate-based herbicides on zebrafish. The results showed that both substances can cause damage to zebrafish, compromising their survival, reproduction, and maintenance. These findings may be applicable to other fish species and aquatic ecosystems.
Article
Agriculture, Dairy & Animal Science
Sara Jorge, Luis Felix, Benjamin Costas, Ana M. M. Valentim
Summary: Proper housing conditions are important for fish welfare. This study found that environmentally enriched housing conditions had minor effects on zebrafish behavior, but no significant influence on body length and stress hormone levels. It suggests that housing conditions should be considered in future research.
Article
Biochemistry & Molecular Biology
Germano A. B. Lanzarin, Luis M. Felix, Sandra M. Monteiro, Jorge M. Ferreira, Paula A. Oliveira, Carlos Venancio
Summary: This study investigated the anti-inflammatory, antioxidant, and anti-apoptotic effects of the plant-based products Thymol (THY) and 24-epibrassinolide (24-EPI). Using zebrafish larvae as a model, the researchers found that both compounds reduced neutrophil recruitment, exhibited antioxidant effects by reducing reactive oxygen species (ROS) production, and displayed anti-apoptotic effects. Furthermore, the compounds also decreased the levels of nitric oxide (NO). These findings suggest that THY and 24-EPI have potential as anti-inflammatory and antioxidant agents.
Article
Agriculture, Dairy & Animal Science
Mariana Caipira Lei, Luis Felix, Ricardo Cardoso, Sandra Mariza Monteiro, Severiano Silva, Carlos Venancio
Summary: This study investigates the use of saliva and eye infrared thermography as non-invasive methods to detect transport-induced stress in calves. The results show that transport increases salivary cortisol, oxidative stress parameters, and eye temperatures, indicating that saliva and infrared thermography are effective in assessing cows' stress status.
Article
Environmental Sciences
Ondina Ribeiro, Claudia Ribeiro, Luis Felix, Isabel Gaivao, Joao Soares Carrola
Summary: This study evaluated the potential effects of 3-MMC on zebrafish embryonic development, behavior, and DNA integrity. The results showed that at low concentrations, 3-MMC did not have expected harmful effects on critical development stages of fish.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)