Journal
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Volume 55, Issue 12, Pages 8180-8190Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.1c01132
Keywords
lipidomics; cell culture; water quality; environmental monitoring; organelles
Categories
Funding
- U.S. EPA Great Lakes National Program Office
- NRC Research Associateship award at the U.S. EPA Center for Environmental Measurement and Modeling
- U.S. Department of Energy
- U.S. EPA
- U.S. EPA Chemical Safety for Sustainability Research Program
- USGS Toxic Substances Hydrology Program
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This study utilized a zebrafish liver cell-based lipidomics approach to assess the ecotoxicological effects of complex contaminant mixtures in impacted stream waters, revealing lipid changes as potential indicators for environmental monitoring and risk assessments.
Surface waters often contain a variety of chemical contaminants potentially capable of producing adverse outcomes in both humans and wildlife due to impacts from industrial, urban, and agricultural activity. Here, we report the results of a zebrafish liver (ZFL) cell-based lipidomics approach to assess the potential ecotoxicological effects of complex contaminant mixtures using water collected from eight impacted streams across the United States mainland and Puerto Rico. We initially characterized the ZFL lipidome using high resolution mass spectrometry, resulting in the annotation of 508 lipid species covering 27 classes. We then identified lipid changes induced by all streamwater samples (nonspecific stress indicators) as well as those unique to water samples taken from specific streams. Subcellular impacts were classified based on organelle-specific lipid changes, including increased lipid saturation (endoplasmic reticulum stress), elevated bis(monoacylglycero)phosphate (lysosomal overload), decreased ubiquinone (mitochondrial dysfunction), and elevated ether lipids (peroxisomal stress). Finally, we demonstrate how these results can uniquely inform environmental monitoring and risk assessments of surface waters.
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