Article
Cell Biology
Kulbhushan Sharma, Nagham T. Asp, Sean P. Harrison, Richard Siller, Saphira F. Baumgarten, Swapnil Gupta, Maria E. Chollet, Elisabeth Andersen, Gareth J. Sullivan, Anne Simonsen
Summary: This study investigates the role of autophagy in early events of lineage commitment using human embryonic and induced pluripotent stem cells. They found that modulation of autophagy affects SOX2 levels and lineage commitment, with autophagy promoting mesendoderm formation and inhibiting neuroectoderm formation. The results suggest that autophagy-mediated SOX2 turnover is a key factor in determining lineage commitment.
Article
Cell Biology
Arindam Ray, Kajal Kamat, Maneesha S. Inamdar
Summary: Mitochondria are dynamic organelles that play a crucial role in blood stem and progenitor cell biology. The protein Asrij/OCIAD1 regulates mitochondrial morphology, energy metabolism, and cell differentiation in both human embryonic stem cells and Drosophila hematopoiesis. Studies in both model systems suggest a conserved role for Asrij in linking mitochondrial dynamics and progenitor differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Claire Fielding, Andres Garcia-Garcia, Claudia Korn, Stephen Gadomski, Zijian Fang, Juan L. Reguera, Jose A. Perez-Simon, Berthold Gottgens, Simon Mendez-Ferrer
Summary: Cholinergic signals in the bone marrow preserve haematopoietic stem cell quiescence and self-renewal under proliferative stress.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Maria Figueres-Onate, Rebeca Sanchez-Gonzalez, Laura Lopez-Mascaraque
Summary: Understanding the strategies used to study cell lineages in the brain and their role in deciphering functional clonal relationships among neural cells; future perspectives will focus on studying cell heterogeneity and the origins of different cell lineages.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Yiran Meng, Joana Carrelha, Roy Drissen, Xiying Ren, Bowen Zhang, Adriana Gambardella, Simona Valletta, Supat Thongjuea, Sten Eirik Jacobsen, Claus Nerlov
Summary: The lymphoid fate in haematopoietic stem cells is determined through epigenetic programming rather than transcriptional control, which also influences the differentiation pathways of platelets with distinct kinetics.
NATURE CELL BIOLOGY
(2023)
Article
Cell Biology
Jun Shen, Yaoyao Zhu, Shuo Zhang, Shuzhen Lyu, Cuicui Lyu, Zicen Feng, Dixie L. Hoyle, Zack Z. Wang, Tao Cheng
Summary: Objectives Vitronectin (VTN) has been shown to promote efficient haematopoietic differentiation of human pluripotent stem cells (hPSCs) by promoting the fate determination of endothelial-haematopoietic lineage during mesoderm development. Signals through alpha v beta 3 and alpha v beta 5 integrins are required for VTN-promoted haematopoietic differentiation. Knockdown experiments confirmed that alpha v beta 3 and alpha v beta 5 act synergistically for early haematopoietic differentiation.
CELL PROLIFERATION
(2021)
Article
Multidisciplinary Sciences
Xuan Zheng, Max A. Betjes, Pascal Ender, Yvonne J. Goos, Guizela Huelsz-Prince, Hans Clevers, Jeroen S. van Zon, Sander J. Tans
Summary: This study developed a new tool called TypeTracker, which identifies cell fates by AI-enabled cell tracking and propagates end point fates back along the branched lineage trees. The research found that cells commit to their new type early, when still deep inside the crypt, contrary to the conventional belief that cells differentiate as they move up the crypt-villus axis. This study raises new questions about the underlying commitment and sorting mechanisms.
Article
Multidisciplinary Sciences
Zhoufeng Wang, Zhe Li, Kun Zhou, Chengdi Wang, Lili Jiang, Li Zhang, Ying Yang, Wenxin Luo, Wenliang Qiao, Gang Wang, Yinyun Ni, Shuiping Dai, Tingting Guo, Guiyi Ji, Minjie Xu, Yiying Liu, Zhixi Su, Guowei Che, Weimin Li
Summary: This study analyzed the composition and progression of LUAD in patient samples using single-cell RNA-seq and multiplex imaging, revealing a potential transcriptional divergence from alveolar type 2 cells. The research identified a group of cells resembling AT2 cells in LUAD progression and detected upregulated genes related to energy metabolism and ribosome synthesis in early stages of LUAD. Potential biomarkers including MDK and TIMP1 were identified for understanding LUAD pathogenesis, shedding light on the transcriptional signatures of distinct histologic stages of LUAD progression that may facilitate early diagnosis.
NATURE COMMUNICATIONS
(2021)
Review
Cell & Tissue Engineering
Nan Hu, Linqing Zou, Cheng Wang, Guoqi Song
Summary: RUNX1T1 plays important roles in acute myeloid leukemia, neuronal development, intestinal development, adipogenesis, angiogenesis, and carcinogenesis, affecting cell fate through transcriptional regulation and signaling pathways.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Lemonia Chatzeli, Ignacio Bordeu, Seungmin Han, Sara Bisetto, Zahra Waheed, Bon-Kyoung Koo, Maria P. Alcolea, Benjamin D. Simons
Summary: The development of the mouse salivary gland involves a process of branching morphogenesis, in which progenitor cells transition from a multipotent state to unipotent states via two distinct bipotent states. Notch signaling and Kras activation play important roles in promoting specific lineages. These findings provide insights into the mechanisms underlying lineage segregation and ductal patterning.
DEVELOPMENTAL CELL
(2023)
Article
Multidisciplinary Sciences
SeungHye Han, Minho Lee, Youngjin Shin, Regina Giovanni, Ram P. Chakrabarty, Mariana M. Herrerias, Laura A. Dada, Annette S. Flozak, Paul A. Reyfman, Basil Khuder, Colleen R. Reczek, Lin Gao, Jose Lopez-Barneo, Cara J. Gottardi, G. R. Scott Budinger, Navdeep S. Chandel
Summary: Alveolar epithelial type 1 (AT1) cells are necessary for gas exchange, while alveolar epithelial type 2 (AT2) cells play a role in lung development and repair. In this study, deleting a specific subunit of the mitochondrial electron transport chain complex I in lung epithelial cells led to abnormal alveolar development and death. Restoring NAD(+) regeneration was able to correct the developmental defects and prevent lethality. These findings highlight the importance of mitochondrial complex I-dependent NAD(+) regeneration in directing cell fate during lung development.
Article
Multidisciplinary Sciences
Xia Chen, Peiliang Wang, Hui Qiu, Yonglin Zhu, Xingwu Zhang, Yaxuan Zhang, Fuyu Duan, Shuangyuan Ding, Jianying Guo, Yue Huang, Jie Na
Summary: This study provides a comprehensive analysis of the epigenetic landscape of human pluripotent stem cell hematopoietic differentiation and identifies JUNB as an essential transcription factor for hemogenic endothelium development.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Shou-Wen Wang, Michael J. Herriges, Kilian Hurley, Darrell N. Kotton, Allon M. Klein
Summary: The goal of single-cell genome-wide profiling is to reconstruct dynamic transitions during cell differentiation, disease onset, and drug response. In this study, a robust computational approach called CoSpar was developed to infer cell dynamics from single-cell transcriptomics integrated with lineage tracing. CoSpar identified previously undiscovered early fate biases and predicted key factors involved in fate choice.
NATURE BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Bin Xue, Dehua Tang, Xin Wu, Zhengyu Xu, Jie Gu, Yueying Han, Zhenshu Zhu, Meng Qin, Xiaoping Zou, Wei Wang, Yi Cao
Summary: This study developed a method to control the mechanical homogeneity of hydrogels and found that the mechanical homogeneity is crucial for controlling the differentiation or stemness maintenance of human embryonic stem cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Genetics & Heredity
Giuliana Ferrari, Adrian J. Thrasher, Alessandro Aiuti
Summary: Haematopoietic stem and progenitor cell (HSPC) gene therapy has shown to be effective in treating monogenic disorders of the blood system, including primary immunodeficiencies and beta-thalassaemia. Additionally, site-specific genome modification of HSPCs using gene editing techniques has great clinical promise.
NATURE REVIEWS GENETICS
(2021)
Review
Hematology
Sam Watcham, Iwo Kucinski, Berthold Gottgens
Article
Cardiac & Cardiovascular Systems
Viktoria Kalna, Youwen Yang, Claire R. Peghaire, Karen Frudd, Rebecca Hannah, Aarti Shah, Lourdes Osuna Almagro, Joseph J. Boyle, Berthold Gottgens, Jorge Ferrer, Anna M. Randi, Graeme M. Birdsey
CIRCULATION RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Julio Sainz de Aja, Sergio Menchero, Isabel Rollan, Antonio Barral, Maria Tiana, Wajid Jawaid, Itziar Cossio, Alba Alvarez, Gonzalo Carreno-Tarragona, Claudio Badia-Careaga, Jennifer Nichols, Berthold Gottgens, Joan Isern, Miguel Manzanares
Article
Hematology
Fiona K. Hamey, Berthold Gottgens
EXPERIMENTAL HEMATOLOGY
(2019)
Article
Cell Biology
Tsuyoshi Fukushima, Yosuke Tanaka, Fiona K. Hamey, Chih-Hsiang Chang, Toshihiko Oki, Shuhei Asada, Yasutaka Hayashi, Takeshi Fujino, Taishi Yonezawa, Reina Takeda, Kimihito Cojin Kawabata, Tomofusa Fukuyama, Terumasa Umemoto, Keiyo Takubo, Hitoshi Takizawa, Susumu Goyama, Yasushi Ishihama, Hiroaki Honda, Berthold Gottgens, Toshio Kitamura
Article
Cell Biology
Fang Dong, Sha Hao, Sen Zhang, Caiying Zhu, Hui Cheng, Zining Yang, Fiona K. Hamey, Xiaofang Wang, Ai Gao, Fengjiao Wang, Yun Gao, Ji Dong, Chenchen Wang, Jinyong Wang, Yu Lan, Bing Liu, Hideo Ema, Fuchou Tang, Berthold Gottgens, Ping Zhu, Tao Cheng
NATURE CELL BIOLOGY
(2020)
Review
Multidisciplinary Sciences
Jong-Eun Park, Laura Jardine, Berthold Gottgens, Sarah A. Teichmann, Muzlifah Haniffa
Article
Allergy
Fiona K. Hamey, Winnie W. Y. Lau, Iwo Kucinski, Xiaonan Wang, Evangelia Diamanti, Nicola K. Wilson, Berthold Gottgens, Joakim S. Dahlin
Summary: The study explored the differentiation pathways of basophils and mast cells at the single-cell level in mice using multicolor flow cytometry, high-coverage single-cell RNA sequencing, and cell fate assays. The results provided a detailed map of differentiation and revealed gene expression changes during the process. Flow cytometry and transcriptional profiling successfully predicted the bipotent phenotype of a previously uncharacterized population of peritoneal basophil-mast cell progenitors.
Article
Cell Biology
Luke T. G. Harland, Claire S. Simon, Anna D. Senft, Ita Costello, Lucas Greder, Ivan Imaz-Rosshandler, Berthold Gottgens, John C. Marioni, Elizabeth K. Bikoff, Catherine Porcher, Marella F. T. R. de Bruijn, Elizabeth J. Robertson
Summary: The study shows that the T-box transcription factor Eomes influences chromatin accessibility at SCL-bound enhancers in the extra-embryonic mesoderm, regulating erythropoiesis and Runx1 expression. Eomes governs the haemogenic competency of ExM and is essential for the normal development of haemogenic endothelium. The findings suggest that haemogenic competence is established earlier during embryonic development than previously thought, with Eomes playing a crucial role in the transcriptional hierarchy of early blood formation.
NATURE CELL BIOLOGY
(2021)
Article
Oncology
Xiaonan Wang, Carlotta Peticone, Ekaterini Kotsopoulou, Berthold Gottgens, Fernando J. Calero-Nieto
Summary: This study used single-cell transcriptomic analysis to characterize the molecular features of CAR T cell activation, revealing several subpopulations of cells in CAR products with reproducible cellular composition across donors. Targeted data interrogation also showed that a small proportion of antigen-responding CAR-expressing cells exhibited exhaustion signatures with known markers and previously unassociated genes.
Correction
Cell Biology
Luke T. G. Harland, Claire S. Simon, Anna D. Senft, Ita Costello, Lucas Greder, Ivan Imaz-Rosshandler, Berthold Gottgens, John C. Marioni, Elizabeth K. Bikoff, Catherine Porcher, Marella F. T. R. de Bruijn, Elizabeth J. Robertson
NATURE CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Pawel Roszak, Jung-Ok Heo, Bernhard Blob, Koichi Toyokura, Yuki Sugiyama, Maria Angels de Luis Balaguer, Winnie W. Y. Lau, Fiona Hamey, Jacopo Cirrone, Ewelina Madej, Alida M. Bouatta, Xin Wang, Marjorie Guichard, Robertas Ursache, Hugo Tavares, Kevin Verstaen, Jos Wendrich, Charles W. Melnyk, Yoshihisa Oda, Dennis Shasha, Sebastian E. Ahnert, Yvan Saeys, Bert De Rybel, Renze Heidstra, Ben Scheres, Guido Grossmann, Ari Pekka Mahonen, Philipp Denninger, Berthold Gottgens, Rosangela Sozzani, Kenneth D. Birnbaum, Yrjo Helariutta
Summary: In plant meristems, tissue-wide maturation gradients in coordination with specialized cell networks establish developmental phases for indeterminate growth. Single-cell transcriptomics was used to reconstruct the developmental trajectory of protophloem in Arabidopsis thaliana roots, revealing the role of PHLOEM EARLY DNA-BINDING-WITH-ONE-FINGER (PEAR) transcription factors in lineage bifurcation and differentiation.
Article
Multidisciplinary Sciences
Daniel Prins, Hyun Jung Park, Sam Watcham, Juan Li, Michele Vacca, Hugo P. Bastos, Alexander Gerbaulet, Antonio Vidal-Puig, Berthold Gottgens, Anthony R. Green
Article
Biochemistry & Molecular Biology
Iwo Kucinski, Nicola K. Wilson, Rebecca Hannah, Sarah J. Kinston, Pierre Cauchy, Aurelie Lenaerts, Rudolf Grosschedl, Berthold Goettgens
Article
Biotechnology & Applied Microbiology
F. Alexander Wolf, Fiona K. Hamey, Mireya Plass, Jordi Solana, Joakim S. Dahlin, Berthold Gottgens, Nikolaus Rajewsky, Lukas Simon, Fabian J. Theis