Journal
FEBS LETTERS
Volume 590, Issue 3, Pages 376-386Publisher
WILEY
DOI: 10.1002/1873-3468.12066
Keywords
conjugation; F-plasmid; Hydrogen-deuterium exchange; thioredoxin fold; type IV secretion
Funding
- Natural Sciences & Engineering Council of Canada (NSERC)
- Canadian Foundation for Innovation (CFI) and York University
- NSERC and York University
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Conjugative DNA transfer by the F-plasmid is achieved through a type IV secretion system (T4SS) encoded within the plasmid's transfer region; TraF is one of several F-T4SS proteins essential for F-pilus assembly. In order to identify regions of the protein important for TraF function, a series of deletion mutants were assessed for their ability to recover conjugative transfer in a traF knockout. Interestingly, modification of any region of TraF abolishes pilus synthesis, resulting in a loss of rescue of conjugative function. Dynamic analysis of TraF by time-resolved hydrogen-deuterium exchange revealed that the C-terminal region containing the predicted thioredoxin-like domain is quite structured, while the N-terminal region, predicted to interact with TraH in the intact F-T4SS, was more dynamic.
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