Article
Microbiology
Francisco Olivenca, Alexandra Nunes, Rita Macedo, David Pires, Catia Silveiro, Elsa Anes, Maria Miragaia, Joao Paulo Gomes, Maria Joao Catalao
Summary: This study reveals the differential phenotypes of M. tuberculosis to beta-lactam antibiotics and finds that highly drug-resistant sublineages are more susceptible to beta-lactams. This provides an important strategy for directing beta-lactams to treat specific sublineages of M. tuberculosis infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yesha Patel, Vijay Soni, Kyu Y. Rhee, John D. Helmann
Summary: Bacteria adapt to stress through mutations in RNA polymerase core subunits, leading to beneficial changes in transcription. Mutations in the rifampicin resistance-determining region (RRDR) of rpoB reduce antibiotic binding and can have pleiotropic effects on bacterial fitness.
Review
Microbiology
Lekshmi Narendrakumar, Medha Chakraborty, Shashi Kumari, Deepjyoti Paul, Bhabatosh Das
Summary: Beta-lactam antibiotics are widely used antimicrobial agents, but their misuse has led to the emergence of resistance in clinically important bacterial pathogens. This review summarizes the success stories of beta-lactamase inhibitors and prospective beta-lactam potentiators, and discusses the challenges in translating these potentiation strategies from bench to bedside. Other mechanisms to reduce global antimicrobial resistance burden are also explored.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Francisco Olivenca, Claudia Ferreira, Alexandra Nunes, Catia Silveiro, Madalena Pimentel, Joao Paulo Gomes, Maria Joao Catalao
Summary: Beta-lactams have been traditionally excluded from tuberculosis therapy due to the intrinsic resistance of Mycobacterium tuberculosis to this antibiotic class. However, recent studies have shown promising results of beta-lactam/beta-lactamase inhibitor combinations in clinical strains. Further research on the mechanisms of action and resistance of these antibiotics and other inhibitors of peptidoglycan synthesis is crucial to determine their place in alternative regimens against drug-resistant strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Annie H. Lee, Rachit Gupta, Hong Nhi Nguyen, Isabella R. Schmitz, Deborah A. Siegele, Pushkar P. Lele
Summary: Difficult-to-eradicate bacterial infections can be treated by sequential exposure to different antibiotics. However, this approach may lead to the adaptation and tolerance of bacterial cells. In this study, we investigated the motility of Escherichia coli cells that adapted to lethal doses of antibiotics. We found that cells with high proton motive force (PMF) were able to swim rapidly in the presence of ampicillin, suggesting that adaptive cells may employ different mechanisms to survive antibiotic stress.
Article
Chemistry, Medicinal
Rachel Berndsen, Taylor Cunningham, Lauren Kaelin, Makayla Callender, W. Dexter Boldog, Brianna Viering, Ashley King, Najwa Labban, Julie A. Pollock, Heather B. Miller, Meghan S. Blackledge
Summary: Antibiotic-resistant infections are a global concern causing millions of deaths annually. Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-acquired infections and is increasingly responsible for difficult-to-treat community-acquired infections. Antibiotic adjuvants, small molecules that enhance antibiotic efficacy through non-toxic mechanisms, show promise as novel therapeutics. Screening for low-molecular-weight compounds identified brominated carbazoles as lead compounds that potentiate beta-lactam antibiotics against medically relevant strains of MRSA by dampening the transcription of key resistance genes. These lead compounds represent novel scaffolds for the development of antibiotic adjuvants.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Infectious Diseases
Caterina Aurilio, Pasquale Sansone, Manlio Barbarisi, Vincenzo Pota, Luca Gregorio Giaccari, Francesco Coppolino, Alfonso Barbarisi, Maria Beatrice Passavanti, Maria Caterina Pace
Summary: Carbapenem antibiotics are highly effective for the treatment of infections caused by resistant bacteria. The widespread use of these antibiotics has resulted in carbapenem resistance, which can occur through both intrinsic and acquired mechanisms.
Article
Microbiology
Alba Ruedas-Lopez, Isaac Alonso-Garcia, Cristina Lasarte-Monterrubio, Paula Guijarro-Sanchez, Eva Gato, Juan Carlos Vazquez-Ucha, Juan Andres Vallejo, Pablo Arturo Fraile-Ribot, Begona Fernandez-Perez, David Velasco, Jose Maria Gutierrez-Urbon, Marina Oviano, Alejandro Beceiro, Concepcion Gonzalez-Bello, Antonio Oliver, Jorge Arca-Suarez, German Bou
Summary: This study aimed to analyze the resistance mechanisms of ceftolozane-tazobactam and ceftazidime-avibactam in multidrug-resistant or extensively drug-resistant P. aeruginosa isolates. The findings revealed that most of the resistant isolates were recovered after treatment with ceftolozane-tazobactam or ceftazidime-avibactam. Chromosomal mutations and specific gene substitutions were identified as the main resistance mechanisms. The study emphasized the potential selection of MDR/XDR P. aeruginosa strains producing certain variants or metallo-beta-lactamases by cephalosporin/beta-lactamase inhibitors, and encouraged judicious use of these agents.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Infectious Diseases
Davide Carcione, Claudia Siracusa, Adela Sulejmani, Valerio Leoni, Jari Intra
Summary: Beta-lactams are important antibiotics, but increasing resistance, especially among Gram-negative bacteria, poses a major threat to public health. Combining beta-lactam antibiotics with beta-lactamase inhibitors can help overcome resistance, but some beta-lactamases are resistant to existing inhibitors, leading to a need for new and more efficient inhibitors.
Article
Microbiology
Christopher Campbell, Claire Fingleton, Merve S. Zeden, Emilio Bueno, Laura A. Gallagher, Dhananjay Shinde, Jongsam Ahn, Heather M. Olson, Thomas L. Fillmore, Joshua N. Adkins, Fareha Razvi, Kenneth W. Bayles, Paul D. Fey, Vinai C. Thomas, Felipe Cava, Geremy C. Clair, James P. 'Gara
Summary: Mutations in the TCA cycle gene sucC increased susceptibility to beta-lactam antibiotics in MRSA, accompanied by accumulation of succinyl-CoA and perturbation of lysine succinylation in the proteome. Interference with the MRSA succinylome may help overcome beta-lactam resistance through controlling cell wall phenotypes.
Article
Infectious Diseases
Aura Falco, Miguel angel Villaquiran-Muriel, Jose David Gallo Perez, Alejandra Mondragon-Quiguanas, Carlos Aranaga, Adriana Correa, Michal Zloch
Summary: This study evaluated the antibiotic resistance of Vibrio metschnikovii and Vibrio injensis isolates from landfill leachates in southwestern Colombia. The results showed susceptibility to ciprofloxacin and imipenem, but resistance to beta-lactams and aminoglycosides. Genomic sequencing revealed genes associated with virulence in one isolate.
Article
Infectious Diseases
Jasmine R. Anderson, Nghi B. Lam, Jazmyne L. Jackson, Sean M. Dorenkott, Taylor Ticer, Emir Maldosevic, Amanda Velez, Megan R. Camden, Terri N. Ellis
Summary: Bacterial exposure to sub-MIC concentrations of cephalothin resulted in the rapid evolution of resistance without acquiring beta-lactamase gene. The resistance exceeded 125 μg/mL and was associated with genetic changes in the RNA polymerase subunit, regulator and sugar transferase. The study demonstrates the significant impact of low sub-MIC concentrations of antibiotics on bacterial evolution of resistance and the possibility of achieving beta-lactam resistance through specific mutations.
Article
Microbiology
Miranda J. Wallace, Sophonie Jean, Meghan A. Wallace, Carey-Ann D. Burnham, Gautam Dantas
Summary: This study demonstrates species-specific patterns in antimicrobial resistance within the Bacteroides fragilis group (BFG) by using comparative genomics, validating new strategies for species-level organism identification and phenotypic resistance prediction in a routine clinical laboratory setting. The research emphasizes the importance of understanding and managing anaerobic infections, as well as the need for further investigations into the molecular basis for species-specific resistance patterns.
Article
Microbiology
Gregory S. Canfield, Anushila Chatterjee, Juliel Espinosa, Mihnea R. Mangalea, Emma K. Sheriff, Micah Keidan, Sara W. McBride, Bruce D. McCollister, Howard C. Hang, Breck A. Duerkop
Summary: Enterococcus faecium, a commensal of the human intestine, has evolved into a hospital-adapted, multidrug-resistant pathogen. Bacteriophages, natural predators of bacteria, show potential as therapeutics against MDR E. faecium infections, despite the unknown molecular events governing their interactions. While phage resistance may emerge, it is shown that lytic phages could effectively synergize with antibiotics to slow or inhibit E. faecium growth.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Maria Montaner, Silvia Lopez-Arguello, Antonio Oliver, Bartolome Moya
Summary: The lack of effective treatment options against Pseudomonas aeruginosa is a major contributor to the silent pandemic. This study provides insights into the mechanisms of action of beta-lactam antibiotics and beta-lactamase inhibitors in P. aeruginosa PAO1, aiding in the design of optimized therapies.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Ekaterina V. Filippova, Misty L. Kuhn, Jerzy Osipiuk, Olga Kiryukhina, Andrzej Joachimiak, Miguel A. Ballicora, Wayne F. Anderson
JOURNAL OF MOLECULAR BIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Ekaterina V. Filippova, Steven Weigand, Jerzy Osipiuk, Olga Kiryukhina, Andrzej Joachimiak, Wayne F. Anderson
JOURNAL OF MOLECULAR BIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Ivan I. Vorontsov, George Minasov, Olga Kiryukhina, Joseph S. Brunzelle, Ludmilla Shuvalova, Wayne F. Anderson
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Multidisciplinary Sciences
Linda Hu, Ekaterina Filippova, Joseph Dang, Sergii Pshenychnyi, Jiapeng Ruan, Olga Kiryukhina, Wayne F. Anderson, Misty L. Kuhn, Alan J. Wolfe
Article
Biochemical Research Methods
Ekaterina V. Filippova, Steven Weigand, Olga Kiryukhina, Alan J. Wolfe, Wayne F. Anderson
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2019)
Article
Biochemical Research Methods
John T. Lazar, Ludmilla Shuvalova, Monica Rosas-Lemus, Olga Kiryukhina, Karla J. F. Satchell, George Minasov
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2019)
Correction
Biochemistry & Molecular Biology
Ekaterina V. Filippova, Misty L. Kuhn, Jerzy Osipiuk, Olga Kiryukhina, Andrzej Joachimiak, Miguel A. Ballicora, Wayne F. Anderson
JOURNAL OF MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Monica Rosas-Lemus, George Minasov, Ludmilla Shuvalova, Zdzislaw Wawrzak, Olga Kiryukhina, Nathan Mih, Lukasz Jaroszewski, Bernhard Palsson, Adam Godzik, Karla J. F. Satchell
Article
Multidisciplinary Sciences
Jonathan P. Allen, Egon A. Ozer, George Minasov, Ludmilla Shuvalova, Olga Kiryukhina, Karla J. F. Satchell, Alan R. Hauser
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Monica Rosas-Lemus, George Minasov, Ludmilla Shuvalova, Nicole L. Inniss, Olga Kiryukhina, Joseph Brunzelle, Karla J. F. Satchell
Article
Multidisciplinary Sciences
Steven Weigand, Ekaterina V. Filippova, Olga Kiryukhina, Wayne F. Anderson
Article
Biochemistry & Molecular Biology
Ivan I. Vorontsov, George Minasov, Joseph S. Brunzelle, Ludmilla Shuvalova, Olga Kiryukhina, Frank R. Collart, Wayne F. Anderson
Article
Biochemistry & Molecular Biology
RA Jungmann, O Kiryukhina
JOURNAL OF BIOLOGICAL CHEMISTRY
(2005)