Article
Chemistry, Multidisciplinary
Mariana Sarai Silva-Lopez, Cesar Arturo Ilizaliturri Hernandez, Hugo Ricardo Navarro Contreras, Angel Gabriel Rodriguez Vazquez, Alejandra Ortiz-Dosal, Eleazar Samuel Kolosovas-Machuca
Summary: This study evaluated the ability of Raman spectroscopy technique to discriminate between healthy and premalignant/malignant cervical cells. The results showed increased intensity in Raman spectra of premalignant/malignant cells. The classification efficiency and sensitivity suggest that Raman spectroscopy could be helpful in the screening and diagnosis of premalignant lesions and cervical cancer.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Analytical
Varsha Karunakaran, Valliamma N. Saritha, Adukkadan N. Ramya, Vishnu Priya Murali, Kozhiparambil G. Raghu, Kunjuraman Sujathan, Kaustabh Kumar Maiti
Summary: Ultrasensitive detection of cancer biomarkers via single-cell analysis through Raman imaging shows promise for early diagnosis. This study focuses on using a SERS-tag for the simultaneous detection of p16/K-i67 in squamous cell carcinoma of the cervix, with accurate recognition in different grades of exfoliated cells. The newly developed Raman image-guided SERS imaging offers a practical approach for clinical applications in the detection of cervical cancer.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yeran Bai, Zhongyue Guo, Fatima C. Pereira, Michael Wagner, Ji-Xin Cheng
Summary: A mid-infrared photothermal-fluorescence in situ hybridization (MIP-FISH) platform is developed for simultaneous identification and metabolic analysis of microbes with single-cell resolution and high throughput. By improving MIP imaging, isotopically labeled compounds incorporated into proteins of individual bacterial cells can be sensitively detected, while FISH labeling with rRNA-targeted probes enables the identification of the analyzed cells. In proof-of-concept experiments, MIP-FISH successfully discriminates and identifies labeled bacterial cells within a complex human gut microbiome sample based on the clear spectral red shift in the protein amide I region caused by incorporation of 13C atoms from 13C-labeled glucose. This study opens up new opportunities for single-cell structure-function analyses in microbiology.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Bhupali Deka, Dambarudhar Mohanta, Abhijit Saha
Summary: This study reports on the formation of fractal-like structures with 2D layer support derived from exfoliated WS2 stacks in a cellulose environment. The morphological features and surface topography of the nanocomposite films were studied using high resolution scanning electron microscopy and atomic force microscopy. The development of termite-like, fractal patterns in the nanocomposites can be observed. The results have implications in advanced sensing, antifouling, photo-catalysis, and energy storage.
SURFACES AND INTERFACES
(2022)
Article
Spectroscopy
Zhenping Kang, Yizhe Li, Jie Liu, Cheng Chen, Wei Wu, Chen Chen, Xiaoyi Lv, Fei Liang
Summary: In this study, a 1D hierarchical convolutional neural network (H-CNN) was proposed to accurately identify different stages of cervical cancer using Raman spectroscopy. The H-CNN outperformed traditional methods in terms of recognition accuracy, stability, and sensitivity, with a macro-accuracy of 94.91%. The hierarchical classification method proposed in this study can diagnose patients more accurately and lays the foundation for further research on Raman spectroscopy as a clinical diagnostic method for cervical cancer.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Henriett Halasz, Zoltan Szatmari, Krisztina Kovacs, Miklos Koppan, Szilard Papp, Edina Szabo-Meleg, David Szatmari
Summary: The ionic environment within the nucleoplasm might diverge from the conditions found in the cytoplasm, potentially playing a role in the cellular stress response. The interactions of nuclear actin and actin-binding proteins with apoptosis factors may differ in the nucleoplasm and cytoplasm. The presence of JMY protein in both the cytoplasm and nucleoplasm suggests its involvement in cytoskeletal remodeling and motility regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Computer Science, Information Systems
Xinyu Huang, Lei Zhang, Liwei Liu, Yang Qin, Qiang Fu, Qiong Wu, Rong Yang, Jun-Peng Lv, Zhenhua Ni, Lei Liu, Wei Ji, Yeliang Wang, Xingjiang Zhou, Yuan Huang
Summary: The study reveals a covalent-like quasi-bonding at the interface of the gold film and MoS2 crystal, leading to the suppression of low-frequency Raman modes on the gold film. This interaction presents a special pinning-effect for low-frequency rigid vibration, providing new inspiration for studying interactions between layered materials and solid surfaces.
SCIENCE CHINA-INFORMATION SCIENCES
(2021)
Article
Biochemical Research Methods
Damien Traynor, Isha Behl, Declan O'Dea, Franck Bonnier, Siobhan Nicholson, Finbar O'Connell, Aoife Maguire, Stephen Flint, Sheila Galvin, Claire M. Healy, Cara M. Martin, John J. O'Leary, Alison Malkin, Hugh J. Byrne, Fiona M. Lyng
Summary: The protocol presented in this study outlines the use of Raman spectroscopy for cancer cytopathology diagnosis, covering detailed instructions for sample preparation, data acquisition, preprocessing, and analysis.
Article
Materials Science, Multidisciplinary
Michael Fickert, Mhamed Assebban, Josep Canet-Ferrer, Gonzalo Abellan
Summary: The article introduces a method for preparing antimonene nanosheets and delves into their physical properties and potential applications through studies on thermal response and Raman spectral analysis.
Article
Chemistry, Multidisciplinary
Awsaf Alsulami, Majed Alharbi, Fadhel Alsaffar, Olaiyan Alolaiyan, Ghadeer Aljalham, Shahad Albawardi, Sarah Alsaggaf, Faisal Alamri, Thamer A. Tabbakh, Moh R. Amer
Summary: Recent reports indicate promising results of thermal and thermoelectric properties in emerging 2D materials, particularly Zirconium-based chalcogenides like ZrS2, ZrSe2, ZrS3, and ZrSe3. This study investigated the thermal properties of these materials using confocal Raman spectroscopy. The Raman signatures of exfoliated ZrX2 (where X = S or Se) showed two distinctive modes, aligned with existing literature for 2D-ZrX2 and identical to exfoliated ZrX3 nanosheets for quasi 1D-ZrX2. The study also measured the temperature dependence of Raman modes, revealing a blueshift for A1g mode in 2D-ZrS2. Additionally, phonon dynamics under optical heating were measured, showing lower interfacial thermal conductance for quasi 1D-ZrX2 due to phonon confinement. These exceptional thermal properties make Zirconium-based materials ideal for thermoelectric device applications and future thermal management strategies.
Article
Biochemistry & Molecular Biology
Ewa Pieta, Karolina Chrabaszcz, Katarzyna Pogoda, Klaudia Suchy, Czeslawa Paluszkiewicz, Wojciech M. Kwiatek
Summary: Despite a lack of treatment options for recurring or persistent cervical cancers, adaptogens have emerged as promising drug sources due to their ability to target multiple molecular processes. This study uses high-definition vibrational spectroscopy methods to evaluate the effectiveness of the adaptogenic drug withaferin A (WFA) in inhibiting cervical cancer cells. The results demonstrate the potential of WFA in inhibiting cancer cell proliferation, providing comprehensive information on the sensitivity of cells to adaptogenic drugs and the nature of adaptogen-induced changes in cancer cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Chemistry, Multidisciplinary
Mariusz Radtke, Christian Hess
Summary: In this study, an affordable synthesis method and preparation of a 2D SnS2 anode material were reported. The material exhibits high cycling durability and its metalation mechanism was investigated using operando Raman spectroelectrochemistry. The results showed that the material has good performance in alkali metal batteries and different mechanisms were observed for the de-/intercalation of lithium, sodium, and potassium.
FRONTIERS IN CHEMISTRY
(2022)
Article
Energy & Fuels
Oktaviardi Bityasmawan Abdillah, Octia Floweri, Muhammad Alief Irham, Akfiny Hasdi Aimon, Takashi Ogi, Ferry Iskandar
Summary: In this study, the structure of electro-exfoliated graphene (EG) was regulated using ultrasonication to improve its electrochemical properties as a supercapacitor electrode. The results showed that ultrasonication treatment increased the exfoliation degree and lateral size of EG sheets, leading to enhanced specific capacitance and cycling performance of the electrode.
Article
Engineering, Electrical & Electronic
Rahul, Sunil K. Arora
Summary: Raman measurements were conducted on exfoliated WSe2 nanosheets supported on silicon substrate in the temperature range from 81 K to 433 K. An analytical model incorporating volume and temperature effects quantitatively revealed the nonlinear temperature dependence of the A(1g) Raman mode. Results showed that the first-order temperature coefficient of the A(1g) mode was -0.00695 +/- 5.719 x 10(-4) cm(-1) K-1, indicating that anharmonic effects such as thermal expansion and three-phonon scattering govern the vibrational characteristics of transition-metal dichalcogenides. These findings are expected to enhance understanding of the anharmonic behavior of phonons in WSe2 nanosheets for potential optoelectronic applications.
JOURNAL OF ELECTRONIC MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Cora Bartus Pravda, Timea Hegedus, Eliezer Fernando Oliveira, Daniel Berkesi, Akos Szamosvolgyi, Zoltan Konya, Robert Vajtai, Akos Kukovecz
Summary: The study demonstrates that using hexagonal boron nitride nanosheets to cover phosphorene can effectively prevent the oxidation sensitivity of phosphorene under ambient conditions, with real-time monitoring of the oxidation process using Raman spectroscopy. The experimental results show that the charge distribution of hexagonal boron nitride can prevent the oxidation reaction and maintain the stability of phosphorene.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Oncology
Damien Traynor, Shiyamala Duraipandian, Ramya Bhatia, Kate Cuschieri, Prerna Tewari, Padraig Kearney, Tom D'Arcy, John J. O'Leary, Cara M. Martin, Fiona M. Lyng
Summary: Raman spectroscopy has shown potential clinical utility for detecting cervical precancer, providing a rapid, label-free, and nondestructive measurement of the biochemical fingerprint of cells, tissues, and biofluids.
Article
Oncology
Barbara Tanno, Flavia Novelli, Simona Leonardi, Caterina Merla, Gabriele Babini, Paola Giardullo, Munira Kadhim, Damien Traynor, Dinesh K. R. Medipally, Aidan D. Meade, Fiona M. Lyng, Soile Tapio, Luca Marchetti, Anna Saran, Simonetta Pazzaglia, Mariateresa Mancuso
Summary: Radiation exposure can lead to heart disease in mice, even when only a portion of the body is irradiated. The study suggests that miRNAs released from irradiated cells may play a role in damaging unirradiated cardiac cells. Understanding the effects of radiation exposure on non-cancer diseases is crucial for developing preventive measures.
Article
Biochemistry & Molecular Biology
Ciara Slattery, Khanh Nguyen, Laura Shields, Isabel Vega-Carrascal, Sean Singleton, Fiona M. Lyng, Brendan McClean, Aidan D. Meade
Summary: In this study, Fourier transform Infrared microspectroscopic imaging was used to analyze an in-vitro model of radiotherapeutic treatment for prostate cancer. The study found that spectral analysis can accurately differentiate different irradiation types and provide a comprehensive set of spectral markers to differentiate irradiation mode and cell fate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Endocrinology & Metabolism
Nitin Patil, Orla Howe, Paul Cahill, Hugh J. Byme
Summary: This paper thoroughly discusses the medical relevance of the cellular glycolytic pathway and explores the current state of monitoring and modelling its dynamics. The potential of label-free vibrational microspectroscopic techniques to overcome the limitations of current approaches is presented.
MOLECULAR METABOLISM
(2022)
Article
Marine & Freshwater Biology
Gabriela H. Da Silva, Lidiane Silva Franqui, Marcelo A. De Farias, Vera Lucia S. S. De Castro, Hugh J. Byrne, Diego S. T. Martinez, Regina T. R. Monteiro, Alan Casey
Summary: This study investigated the cytotoxicity, protein corona formation, and cellular internalisation of TiO2_MWCNT on RTG-2 cells for the first time. The results showed that this nanohybrid had no toxicity effects on RTG-2 cells within 24 hours. Spectral and microscopic analysis revealed that TiO2_MWCNT can form complexes through adsorption of proteins and be internalised by cells.
AQUATIC TOXICOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Rubina Shaikh, Amuthachelvi Daniel, Fiona M. Lyng
Summary: This review focuses on recent advances in using Raman spectroscopy for cervical cancer detection and discusses the need for new methods due to the limitations of current screening techniques. The potential of Raman spectroscopy combined with statistical analysis has been shown in detecting cervical cancer. The review examines research using exfoliated cells, biofluid samples, and tissue for Raman spectroscopic approaches.
Article
Biochemistry & Molecular Biology
Shane Forde, Giulianna Vozza, David J. Brayden, Hugh J. Byrne, Jesus M. Frias, Sinead M. Ryan
Summary: In order to address the challenges of oral administration of SeMet, researchers encapsulated it in zein-coated nanoparticles made from chitosan using an ionic gelation formulation. The SeMet-NPs showed high stability and minimal cytotoxicity, as well as antioxidant properties. Additionally, they exhibited increased intestinal permeability compared to free SeMet. These findings suggest the potential of SeMet-NPs for further development as an oral supplement.
Article
Chemistry, Multidisciplinary
Furong Tian, Luis Felipe das Chagas e Silva de Carvalho, Alan Casey, Marcelo Saito Nogueira, Hugh J. Byrne
Summary: In recent years, the disease burden of hyperuricemia has been increasing, especially in high-income countries and the economically developing world with a Western lifestyle. Improved methods of uric acid and hypoxanthine detection using surface-enhanced Raman spectroscopy (SERS) and high-performance liquid chromatography (HPLC) were demonstrated by analyzing three different bodily fluids. Gold nanostars were used for quantitative detection of uric acid and hypoxanthine concentrations. The results show strong correlations between HPLC and SERS, and the potential of vibrational spectroscopy in clinical applications was highlighted.
Article
Pharmacology & Pharmacy
Suha Elderderi, Franck Bonnier, Xavier Perse, Hugh J. Byrne, Florent Yvergnaux, Igor Chourpa, Abdalla A. Elbashir, Emilie Munnier
Summary: Raman spectroscopy is a useful technique for quantifying complex cosmetic products without the need for extensive pre-analytical processing. This study demonstrates the effectiveness of Raman spectroscopy coupled with partial least squares regression (PLSR) in quantifying Alginate nanoencapsulated Piperonyl Esters (ANC-PE) in a hydrogel. By dividing the samples into a training set and a test set, the accuracy of the prediction model was evaluated, with a root mean square error of cross-validation (RMSECV) and prediction (RMSEP) of 0.142% and 0.148% (w/w PE), respectively. These results highlight the potential of Raman spectroscopy for rapid and label-free quantification of active cosmetic ingredients in complex formulations, promising future applications in analytical quality control (AQC).
Article
Chemistry, Analytical
Maria Joao Oliveira, Soraia Caetano, Ana Dalot, Filipe Sabino, Tomas R. Calmeiro, Elvira Fortunato, Rodrigo Martins, Eulalia Pereira, Miguel Prudencio, Hugh J. Byrne, Ricardo Franco, Hugo Aguas
Summary: Early and accurate detection of pathogenic microorganisms is crucial for diagnosis and patient outcomes. The combination of a polystyrene-based microfluidic device and Surface-Enhanced Raman Spectroscopy shows excellent sensitivity and specificity in detecting malaria. The system can be easily adapted for other pathogens and has the potential for early diagnosis of infectious diseases.
Article
Chemistry, Analytical
Valentina Notarstefano, Alessia Belloni, Paolo Mariani, Giulia Orilisi, Giovanna Orsini, Elisabetta Giorgini, Hugh J. J. Byrne
Summary: In this study, Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) and Principal Component Analysis (PCA) were used to analyze Raman MicroSpectroscopy (RMS) data of Oral Squamous Carcinoma Cells (OSCC) exposed to the chemotherapeutic agent cisplatin. The results showed that RMS coupled with multivariate analysis can quantitatively monitor the progression of cellular responses to different drugs, providing potential applications for label-free, in vitro, pre-clinical screening.
Review
Endocrinology & Metabolism
Zohreh Mirveis, Orla Howe, Paul Cahill, Nitin Patil, Hugh J. J. Byrne
Summary: In recent years, the analysis of the glutamine metabolic pathway has been a major focus in metabolomics research due to its significance in cell biosynthesis and bioenergetics, especially in cancer cell survival. However, monitoring the kinetics of this pathway in a living cell in real-time and holistically remains a challenge.
Article
Chemistry, Analytical
Jade F. Monaghan, Daniel Cullen, Claire Wynne, Fiona M. Lyng, Aidan D. Meade
Summary: This study evaluated the impact of pre-analytical procedures on the spectral integrity of lymphocytes analyzed using FTIR and Raman spectroscopy. The findings showed that the freeze-thaw cycle, in vitro culture inter-operator variability, and the time to lymphocyte isolation can affect the spectral signature of lymphocytes. However, the lack of standardized protocols for sample preparation, storage, and processing hinders the integration of these techniques into clinical workflows.
Article
Materials Science, Multidisciplinary
Aimee Sheehan, Tatsiana Mikulchyk, Catherine S. P. De Castro, Safakath Karuthedath, Wejdan Althobaiti, Metodej Dvoracek, Hugh J. Sabad-e-Gul, Hugh J. Byrne, Frederic Laquai, Izabela Naydenova, Mikhail A. Filatov
Summary: A series of new heavy-atom-free photosensitizers were designed and applied for holographic recording in a photopolymerizable material. The photoinduced electron transfer and spin-orbit charge transfer intersystem crossing were studied, and highly efficient photosensitization was observed.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
