Journal
CHINESE CHEMICAL LETTERS
Volume 33, Issue 3, Pages 1373-1376Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2021.08.024
Keywords
Single-cell pipette; Microfluidic chip; Single-cell capture; ETAAS; ICP-MS; CdTe QDs
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Funding
- National Natural Science Foundation of China [21727811, 21922402]
- Fundamental Research Funds for the Central Universities [N2005003, N2105017]
- Liaoning Revitalization Talents Program [XLYC1802016]
- Scientific Research Funding Project of the Education Department of Liaoning [LJKZ0007]
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Accurate single-cell capture is crucial for single-cell biological and chemical analysis. This study presents a modular single-cell pipette microfluidic chip that enables efficient and precise capture of single viable cells and compatibility with various analytical instruments. The chip holds great potential for applications in single-cell research and analysis.
Accurate single-cell capture is a crucial step for single cell biological and chemical analysis. Conventional single-cell capturing often confront operational complexity, limited efficiency, cell damage, large scale but low accuracy, incompetence in the acquirement of nano-upgraded single-cell liquid. Flow cytometry has been widely used in large-scale single-cell detection, while precise single-cell isolation relies on both a precision operating platform and a microscope, which is not only extremely inefficient, but also not conducive to couple with modern analytical instruments. Herein, we develop a modular single-cell pipette (mSCP) microfluidic chip with high efficiency and strong applicability for accurate direct capture of single viable cell from cell suspensions into nanoliter droplets (30-1000 nL). The mSCP is used as a sampling platform for the detection of CdTe quantum dots in single cells with electrothermal atomic absorption spectrometry (ETAAS) for the first time. It also ensures precise single-cell sampling and detection by inductively coupled plasma mass spectrometry (ICP-MS). (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
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