Article
Biophysics
Yaqian Chen, Jiaqi Ma, Xuechi Yin, Ziai Deng, Xiaojing Liu, Di Yang, Lei Zhao, Jing Sun, Jianlong Wang, Daohong Zhang
Summary: An immersible amplification dip-stick immunoassay (DSIA) using multifunctional nanocomposites was developed for the sensitive detection of Salmonella typhimurium (S. typhi) and Escherichia coli O157:H7 (E. coli O157:H7). The reporter molecule, CoFe2O4 (CFO), possessed superior catalase-like activity and magnetic properties. It enabled a simple and highly sensitive detection with a dual-visible mode. The DSIA achieved matrix-interference-free detection with a dynamic range of 102-108 CFU/mL and a low assay limit of 102 CFU/mL, and showed reasonable recovery rates for contamination monitoring in milk and beef samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Jiye Li, Jie Wang
Summary: This study demonstrates the use of extinction spectrum to quickly transform the signal of MoS2-NS aggregation and reveals the size-dependent extinction response. Additionally, the size-dependent optical extinction behavior of MoS2-NSs with aptamer-induced dispersion is studied to improve target identification ability. This approach has been successfully employed for label-free detection of Escherichia coli O157:H7.
Article
Chemistry, Analytical
Yuzhi Li, Fei Gao, Chang Lu, Marie-Laure Fauconnier, Jinkai Zheng
Summary: The novel organic and inorganic hybrid Au/Fe3+ nanoclusters exhibited excellent sensitivity and specificity in detecting Escherichia coli O157:H7, making them suitable for food safety monitoring. By combining advanced nanomaterials with biosensing technology, the biosensor achieved sensitive and quantitative detection of the target bacteria.
Article
Agriculture, Dairy & Animal Science
Yuwei Ren, Pingping Gao, Yang Song, Xinyan Yang, Tao Yang, Sihan Chen, Shiqian Fu, Xue Qin, Meili Shao, Chaoxin Man, Yujun Jiang
Summary: A highly sensitive method was developed in this study, combining aptamer-exonuclease III-assisted amplification with lateral flow assay based on gold nanoparticles for visual detection of E. coli O157:H7.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Ting Zheng, Xianming Li, Ya-Ni Xie, Bin Yang, Peng Wu
Summary: This study proposes a dual-gene RPA-LFA method to selectively detect E. coli O157:H7 by recognizing target amplicons and eliminating false positives. The assay shows high sensitivity and specificity for the detection of this pathogen.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Gan Zhang, Hong Hu, Shengliang Deng, Xiaoyue Xiao, Yonghua Xiong, Juan Peng, Weihua Lai
Summary: In this study, bimetallic Ag-Au urchin-like hollow nanospheres (BUHNPs) were developed through a simple and rapid synthesis method. BUHNPs exhibited excellent performance in terms of colorimetric signal brightness, photothermal signal strength, and antibody coupling efficiency. The colorimetric and photothermal LFIA (BUHNPs-CM-LFIA and BUHNPsPT-LFIA) based on BUHNPs were used for the sensitive detection of a pathogenic bacterium (Escherichia coli O157:H7). The detection limit values of the colorimetric and photothermal quantitative analysis were approximately 4-fold and 18-fold lower than that of AuNPs-LFIA, respectively. This study suggests that a dual-readout LFIA with BUHNPs as a promising signal reporter can improve the detection performance of LFIA and establish a more accurate and sensitive detection platform.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Abdus Sobhan, Fei Jia, Lisa Cooney Kelso, Sonatan Kumar Biswas, Kasiviswanathan Muthukumarappan, Changyong Cao, Lin Wei, Yanbin Li
Summary: An activated biochar-based immunosensor was developed to rapidly detect E. coli O157:H7 cells without incubation. The sensor coated with activated biochar and functionalized with antibodies showed enhanced impedance amplitude compared to the control electrode. The immunosensor was capable of detecting E. coli O157:H7 cells with a limit of detection of 4 log CFU/mL.
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biophysics
Dongli Sun, Tingting Fan, Feng Liu, Fangxiu Wang, Dan Gao, Jin-Ming Lin
Summary: A microfluidic chemiluminescence biosensor based on a multiple signal amplification strategy was developed for rapid and ultrasensitive detection of E. coli O157:H7. The biosensor showed excellent recovery rate and selectivity, making it a promising tool for rapid detection of foodborne pathogens.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Xiaocui Lai, Ganggang Zhang, Lifeng Zeng, Xiaoyue Xiao, Juan Peng, Ping Guo, Wei Zhang, Weihua Lai
Summary: The magnetic bimetallic nanozyme Fe3O4@PDA@Pd/Pt was utilized as a probe in immunochromatographic assay to reduce matrix interference and achieve ultrasensitive detection. The PDA coating improved the catalytic ability and stability of the nanozyme, allowing successful detection of biomolecules at low concentrations.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biology
Mokhtar Nosrati, Jafar Amani
Summary: This study utilized machine learning and the PseKNC approach for in silico screening of ssDNA aptamers against Escherichia coli O157:H7. The ANN and RF algorithms showed adequate performances in data classification processes. Increasing data size had a significant impact on the accuracy of the RF model, leading to the selection of RF algorithm for aptamer screening. The selected aptamers by the proposed method exhibited appropriate structure properties and no thermodynamics limit for folding.
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Smriti Shringi, Haiqing Sheng, Andrew A. Potter, Scott A. Minnich, Carolyn J. Hovde, Thomas E. Besser
Summary: Oral immunization with live stx-negative LEE+ E. coli O157:H7 in cattle reduces the colonization of wild-type E. coli O157:H7 strains at the rectoanal junction and shortens the shedding duration.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Food Science & Technology
Man-Seok Choi, Eun Bi Jeon, Ji Yoon Kim, Eun Ha Choi, Jun Sup Lim, Jinsung Choi, Shin Young Park
Summary: The efficacy of dielectric barrier discharge (DBD) plasma treatment for reducing non-pathogenic Escherichia coli and E. coli O157:H7 in oysters was studied. Exposure to DBD plasma for at least 60 minutes resulted in significant reductions in both types of bacteria, with minimal impact on the glycogen content and texture of the oysters. This non-thermal intervention could be a promising method for improving the safety of fresh oysters.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yibo Liu, Juewen Liu
Summary: In this study, a new DNA aptamer (UA-Mg-1) for uric acid detection was obtained through a salt-toggled selection method. The aptamer exhibited strong binding affinity and showed metal dependency only on Mg2+ ions. The UA-Mg-1 aptamer was then engineered into a fluorescent biosensor for uric acid detection.
Article
Chemistry, Multidisciplinary
Yuzhe Ding, Po-Jung Jimmy Huang, Mohamad Zandieh, Jinghan Wang, Juewen Liu
Summary: In this study, the authors compared gold nanoparticles (AuNPs) prepared using different reducing agents and found that citrate-AuNPs and glucose-AuNPs had better DNA adsorption and affinity. Citrate-AuNPs also exhibited higher sensitivity for label-free DNA detection. The authors further investigated the effect of aging on citrate-AuNPs and found that their colloidal stability, DNA adsorption efficiency, and sensing performance decreased gradually. The study highlights the importance of considering the aging effect when using AuNPs in colloidal science and biosensor technology.
Article
Biochemical Research Methods
Javier Perez Tobia, Po-Jung Jimmy Huang, Yuzhe Ding, Runjhun Saran Narayan, Apurva Narayan, Juewen Liu
Summary: Computer-aided prediction of aptamer sequences has traditionally focused on primary sequence alignment and motif comparison. However, this study introduces a new algorithm that incorporates secondary structure information, combining conserved primary sequences and secondary structures. This algorithm was successfully used in predicting aptamer sequences from two different selections and showed promise in predicting challenging sequences. It provides a novel approach to pattern discovery in aptamer selection libraries and has the potential to shorten selection time and improve applications in biosensors and chemical biology.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Huizhen Fan, Jiajia Zheng, Jiaying Xie, Juewen Liu, Xingfa Gao, Xiyun Yan, Kelong Fan, Lizeng Gao
Summary: Nanozymes have great potential as alternatives to natural enzymes, but their practical use is limited by their low catalytic activity compared to natural enzymes. This study employed a surface engineering strategy using charge-transferrable ligands, such as polystyrene sulfonate (PSS), to improve the specific activity of Ru nanozymes. The modified Ru nanozyme exhibited a peroxidase-like specific activity double that of horseradish peroxidase. The modified Ru-peroxidase nanozyme was successfully used to develop an immunoassay with significantly increased detection sensitivity compared to traditional enzyme-linked immunosorbent assay.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Chenqi Niu, Juewen Liu, Xinhui Xing, Chong Zhang
Summary: In this study, a novel strategy was proposed to eliminate the non-specific signal from non-specific amplification by integrating the CRISPR-Cas12a system into two-templates exponential amplification reactions (EXPAR). The developed EXPAR-Cas12a strategy named EXPCas acted as a filter to filter out non-specific amplificons and improved the signal-to-background ratio significantly. With miRNA-21 as the target, the detection could be accomplished in 40 min with a LOD of 103 fM and no non-specific amplification was observed.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Na Wu, Mohamad Zandieh, Ting Yang, Juewen Liu
Summary: The detection of insulin is an important analytical task. Previous studies believed that guanine-rich DNA binds to insulin and a specific insulin aptamer was selected based on this belief. In this study, three different insulin preparation methods were evaluated using fluorescence polarization assays. The results showed that insulin samples containing Zn(2+) did not bind to the aptamer DNA, while insulin monomers and dimers with Zn2+ removed were able to bind. C-rich DNA exhibited stronger binding affinities and faster kinetics compared to the previously reported aptamer. This study provides important insights into the detection of insulin and the binding mechanisms between oligomeric insulin and DNA.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yuheng Song, Derek H. G. von Eppinghoven, Yang Zhou, Hanxiao Zhang, Juewen Liu
Summary: This study investigates the adsorption of tetracyclines to gold nanoparticles and its impact on the design of label-free biosensors. The results provide valuable insights into the interaction between tetracyclines and gold nanoparticles, and offer guidance for the development of aptamer-based biosensors.
CANADIAN JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yuzhe Ding, Juewen Liu
Summary: This article introduces a classical DNA aptamer that has been widely used for biosensing, imaging, and DNA nanotechnology. Two separate selections were performed using adenosine and ATP as targets, obtaining two new aptamers with significantly higher affinity compared to the classical aptamer. The new aptamers bind to one target molecule instead of two, and their secondary structures and specific binding were confirmed through mutation studies.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Mohamad Zandieh, Una E. Hogan, Rodney D. L. Smith, Juewen Liu
Summary: The wettability of microplastics can change due to surface transformations. This study investigated the adsorption of spherical nucleic acids (SNAs) and linear DNA on microplastics to understand their wettability. Soaking microplastics in water for 3 months increased SNA adsorption, while the adsorption of linear DNA remained the same. Reversibility was observed when the soaked microplastics were dried and rehydrated. Heating the microplastics had a similar effect to long-term soaking.
Article
Optics
Yuxuan Qiu, Wei Chen, Yuran Huang, Yueshu Xu, Yile Sun, Tao Jiang, Zhimin Zhang, Longhua Tang, Xiang Hao, Cuifang Kuang, Xu Liu
Summary: In subtraction microscopy, the difference between Gaussian and doughnut point spread functions (PSFs) leads to negative sidelobes and information loss. This trade-off hampers the improvement in performance. Our proposed Deep-IWS algorithm based on deep learning assigns subtractive coefficients adaptively, leading to 1.8 times higher resolution than confocal microscopy and fewer artifacts with higher SNR in reconstructed images.
OPTICS AND LASERS IN ENGINEERING
(2023)
Article
Chemistry, Analytical
Yao Lin, Xueshan Chen, Yuyang Li, Yi Ye, Lin Yang, Linchuan Liao, Yurong Deng, Juewen Liu, Jinyi Zhang, Chengbin Zheng
Summary: A neutral photocatalytic oxidation strategy induced by scandium (Sc3+) was developed for the visual detection of cocaine within 40 s. The strategy was based on G-quadruplex/PPIX and split aptamers, allowing the activation of Sc3+-mediated photosensitization oxidation of TMB.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Hanxiao Zhang, Albert Zehan Li, Juewen Liu
Summary: Using DNA staining dyes for label-free detection of aptamer binding has been done for a long time, but the cationic nature of the dyes can lead to unstable signals and false interpretations of results due to adsorption. This study evaluated the stability of the signal and found that a quartz cuvette provided a more stable signal compared to a polystyrene microplate, and equilibrating the system improved signal stability. The surfactant Triton X-100 was found to have the best protective effect against fluorescence signal decrease due to dye adsorption, and its use improved signal stability for different aptamers.
Article
Chemistry, Analytical
Bangrui Shao, Qiuxiang He, Tao Jiang, Biaofeng Zeng, Cuifang Kuang, Xu Liu, Longhua Tang
Summary: In this study, we report a feedback-controlled electrochemical etching approach to fabricate tunneling electrodes with a controlled nanogap, which are successfully applied in single-molecule sensing.
Article
Biochemical Research Methods
Tyler S. Lott, Ariel A. Petruk, Nicolette A. Shaw, Natalie Hamada, Carmen M. Andrei, Yibo Liu, Juewen Liu, German Sciaini
Summary: Liquid cell electron microscopy (LCEM) has suffered from reproducibility issues and limited imaging quality over a wide field of view. In this study, a shape-engineered nanofluidic cell architecture and an air-free drop-casting sample loading technique were introduced to overcome these challenges. The presented LCEM method enables high-resolution imaging without bulging membranes, providing lattice resolution and sufficient contrast for observing unstained liposomes in their near native environment.
Review
Chemistry, Multidisciplinary
Zhen Wang, Man Li, Huaiyu Bu, David S. Zia, Penggao Dai, Juewen Liu
Summary: Biosensors use biomolecules and nanomaterials for target recognition and signal transduction. There are three types of biosensors described in this review: those relying on fluorescent nanomaterials, those based on regulation of catalytic activity of nanozymes, and those using additional signal transduction molecules. Challenges include limited selectivity and susceptibility to interference, while future research opportunities involve mechanistic studies, improving selectivity, and real sample testing.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)