4.8 Review

Anaerobic co-digestion: Current status and perspectives

Journal

BIORESOURCE TECHNOLOGY
Volume 330, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125001

Keywords

Anaerobic co-digestion; Co-digestate management; Kinetic modeling; Microbial community; Synergistic effect

Funding

  1. Supplemental Grant through the United States Department of Agriculture (USDA)
  2. College of Tropical Agriculture and Human Resources, University of Hawai'i at Manoa

Ask authors/readers for more resources

Co-digestion technology offers enhanced digestibility, improved process stability, and higher nutrient value compared to mono-digestion. A critical comparison of mono-digestion and co-digestion reveals the latter's greater flexibility and sustainability in handling diverse feedstocks.
Anaerobic digestion is a long-established technology for the valorization of diverse organic wastes with concomitant generation of valuable resources. However, mono-digestion (i.e., anaerobic digestion using one feedstock) suffers from challenges associated with feedstock characteristics. Co-digestion using multiple feedstocks provides the potential to overcome these limitations. Significant research and development efforts have highlighted several inherent merits of co-digestion, including enhanced digestibility due to synergistic effects of co-substrates, better process stability, and higher nutrient value of the produced co-digestate. However, studies focused on the underlying effects of diverse co-feedstocks on digester performance and stability have not been synthesized so far. This review fills this gap by highlighting the limitations of mono-digestion and critically examining the benefits of co-digestion. Furthermore, this review discusses synergistic effect of co-substrates, characterization of microbial communities, the prediction of biogas production via different kinetic models, and highlights future research directions for the development of a sustainable biorefinery.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Agricultural Engineering

Anaerobic co-digestion of various organic wastes: Kinetic modeling and synergistic impact evaluation

Renisha Karki, Wachiranon Chuenchart, K. C. Surendra, Shihwu Sung, Lutgarde Raskin, Samir Kumar Khanal

Summary: Anaerobic mono- and co-digestion of coffee pulp, cattle manure, food waste, and dewatered sewage sludge were evaluated using biochemical methane potential tests. Mono-digestion of coffee pulp and food waste yielded higher methane yields compared to the co-digestion ratios examined, with no synergistic or antagonistic effects observed. Nine different kinetic models were compared, with cone and modified Gompertz models identified as best-fit for mono- and co-digestion of coffee pulp and cattle manure, and superimposed model for food waste and dewatered sewage sludge.

BIORESOURCE TECHNOLOGY (2022)

Article Agricultural Engineering

Application of machine learning in anaerobic digestion: Perspectives and challenges

Ianny Andrade Cruz, Wachiranon Chuenchart, Fei Long, K. C. Surendra, Larissa Renata Santos Andrade, Muhammad Bilal, Hong Liu, Renan Tavares Figueiredo, Samir Kumar Khanal, Luiz Fernando Romanholo Ferreira

Summary: This review critically examines the application of machine learning in the anaerobic digestion process, focusing on evaluating important algorithms and their applications in AD modeling, and outlining the challenges faced by ML in this context.

BIORESOURCE TECHNOLOGY (2022)

Article Agronomy

Effects of Straw Biochar on Heavy Metal Cu in Soil Under Different Conditions

Yiping Guo, Xiangkai Tao, Weiyong Zhu, Yapeng Ji, K. C. Surendra, Samir Kumar Khanal, Bingtao Liu, Guoting Li

Summary: Biochar is commonly used as a material to improve soil properties, but its application conditions can influence the adsorption of heavy metal copper. This study found that a specific combination of biochar, wheat straw, and cow manure compost could greatly enhance the adsorption rate of copper in soil. The adsorption processes were identified as multiphase diffusion processes, and the interactions between copper and soil were mainly monolayer adsorption with other complex reactions. However, in leaching experiments, the addition of wheat straw and compost led to more copper being dissolved in the leaching water.

COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS (2023)

Review Agricultural Engineering

Applications of artificial intelligence in anaerobic co-digestion: Recent advances and prospects

Muzammil Khan, Wachiranon Chuenchart, K. C. Surendra, Samir Kumar Khanal

Summary: Anaerobic co-digestion (AcoD) offers various advantages in terms of better digestibility, process stability, and increased methane yield. However, the efficient operation of an AcoD system requires a comprehensive understanding of important operational parameters and monitoring processes can be tedious. Artificial intelligence (AI) has emerged as an innovative approach to optimize and control the AcoD process. This review discusses the applications of AI in AcoD process optimization, control, prediction, and real-time monitoring, as well as the comparison of different AI algorithms.

BIORESOURCE TECHNOLOGY (2023)

Review Environmental Sciences

Nanobubble technology applications in environmental and agricultural systems: Opportunities and challenges

Kyle Rafael Marcelino, Li Ling, Sumeth Wongkiew, Hua Thai Nhan, K. C. Surendra, Ty Shitanaka, Hui Lu, Samir Kumar Khanal

Summary: There has been increasing interest in nanobubble technology due to its unique physicochemical properties, which have potential applications in environmental engineering and agriculture. The use of nanobubbles can inactivate pathogens in water treatment, remove harmful microorganisms on food, and remove pollutants from wastewater. Nanobubbles also have the potential to increase the dissolved oxygen level and plant yields. However, the fundamental mechanisms and mass transfer performance of nanobubble technology are not well understood.

CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY (2023)

Article Engineering, Environmental

Water-energy-greenhouse gas nexus of a novel high-rate activated sludge-two-stage vertical up-flow constructed wetland system for low-carbon wastewater treatment

Mingde Ji, Jie Wang, Samir Kumar Khanal, Saqi Wang, Jian Zhang, Shuang Liang, Huijun Xie, Haiming Wu, Zhen Hu

Summary: In this study, a high-rate activated sludge-two-stage vertical up-flow constructed wetland (HRAS-TVUCW) system was developed to reduce carbon emissions during municipal wastewater treatment. The carbon emissions of the HRAS-TVUCW system were 4.1-fold lower than that of the conventional anaerobic/anoxic/aerobic (A2O) process, while the recovered energy from the HRAS-TVUCW system increased its contribution to carbon neutrality by 4.6-fold. Functional microbial community analysis showed that the controlled dissolved oxygen allocation in the HRAS-TVUCW system led to distinct microbial communities in each unit, facilitating denitrification efficiency increase and carbon emissions reduction. Overall, the HRAS-TVUCW system can be considered as a cost-effective and sustainable low-carbon technology for municipal wastewater treatment.

WATER RESEARCH (2023)

Editorial Material Energy & Fuels

Biotechnology for Resource Efficiency, Energy, Environment, Chemicals, and Health

Ayon Tarafdar, Sunita Varjani, Samir Khanal, Siming You, Ashok Pandey

BIOENERGY RESEARCH (2023)

Editorial Material Agricultural Engineering

Artificial intelligence and machine learning for smart bioprocesses

Samir Kumar Khanal, Ayon Tarafdar, Siming You

Summary: In recent years, there has been significant attention towards the digital transformation of bioprocesses, focusing on interconnectivity, online monitoring, process automation, AI and ML, and real-time data acquisition. AI can analyze and forecast high-dimensional data from bioprocess dynamics, allowing for precise control and synchronization to improve performance and efficiency. Data-driven bioprocessing is a promising technology for addressing challenges in bioprocesses. The MLSB-2022 special issue incorporates recent advances in ML and AI applications in bioprocesses, providing valuable resources for researchers to learn major developments.

BIORESOURCE TECHNOLOGY (2023)

Article Engineering, Environmental

Competitive Reactions during Ethanol Chain Elongation Were Temporarily Suppressed by Increasing Hydrogen Partial Pressure through Methanogenesis Inhibition

Shilva Shrestha, Siqi Xue, Lutgarde Raskin

Summary: Organic waste can be converted into valuable platform chemicals through chain elongation using mixed microbial communities. However, the use of complex microbial communities and the heterogeneity of waste streams can lead to undesirable reactions and decreased process efficiency. This study investigated the use of 2-bromoethanesulfonate (2-BES) to manipulate the hydrogen partial pressure (PH2) and suppress excessive ethanol oxidation to acetate (EEO) in a bioreactor. While initially effective, the addition of 2-BES resulted in the persistence of Methanobrevibacter spp. and the selection of resistant populations over time, indicating the need for alternative methods to maintain optimum PH2 for long-term suppression of EEO.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2023)

Article Environmental Sciences

Use of nanobubble water bioaugmented anaerobically digested sludge for high-efficacy energy production from high-solids anaerobic digestion of corn straw

Xuezhi Wang, Zhongfang Lei, Zhenya Zhang, Kazuya Shimizu, Duu-Jong Lee, Samir Kumar Khanal

Summary: In this study, the use of nanobubble water (NBW) as a bio-augmentation agent in high-solids anaerobic digestion (AD) of corn straw was investigated. The results showed that NBW bio-augmentation significantly increased methane production and achieved a net energy gain and energy recovery, providing a sustainable management solution for renewable energy production from agricultural wastes.

SCIENCE OF THE TOTAL ENVIRONMENT (2023)

Article Biotechnology & Applied Microbiology

Simultaneous carbon catabolite repression governs sugar and aromatic co-utilization in Pseudomonas putida M2

Shilva Shrestha, Deepika Awasthi, Yan Chen, Jennifer Gin, Christopher J. Petzold, Paul D. Adams, Blake A. Simmons, Steven W. Singer

Summary: This study investigated the carbon catabolite repression (CCR) functioning in P. putida M2, a strain capable of consuming sugars and aromatic compounds. The results showed that incomplete substrate consumption occurred during cultivation with a mixture of glucose and aromatic compounds, while transient intermediate accumulation and complete aromatic consumption were observed during xylose-aromatic consumption. Proteomics analysis revealed stronger repression of aromatic catabolic proteins by glucose than xylose. Additionally, CRISPRi-based gene repression experiments showed that reduction of crc expression led to faster growth and improved glucose and p-coumarate uptake. Small RNA sequencing results indicated lower levels of CrcY and CrcZ homologues under strong CCR conditions.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2023)

Article Engineering, Environmental

Evaluation of Nanaerobic Digestion as a Mechanism to Explain Surplus Methane Production in Animal Rumina and Engineered Digesters

Zhuoying Wu, Duc Nguyen, Shilva Shrestha, Lutgarde Raskin, Samir Kumar Khanal, Po-Heng Lee

Summary: We introduced a concept called "nanaerobic digestion", which refers to the enhanced methane production in animal rumen and engineered digesters that is associated with the co-occurrence of methanogens and nanaerobes in a unique ecological niche with very low levels of dissolved oxygen. We analyzed meta-omic datasets and found that animal rumen shares similarities with oxygenated digesters, suggesting that trace levels of oxygen play a role in efficient digestion in ruminants. Prevotellaceae, a bacterial family abundant in rumen, contains many nanaerobes that contribute to efficient methane generation by performing nanaerobic respiration using cytochrome bd oxidase.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2023)

Article Environmental Sciences

Effects of vermicompost leachate on nitrogen, phosphorus, and microbiome in a food waste bioponic system

Sumeth Wongkiew, Chongrak Polprasert, Pongsak (Lek) Noophan, Thammarat Koottatep, Vorapot Kanokkantapong, K. C. Surendra, Samir Kumar Khanal

Summary: Food waste can be combined with bioponics to enhance nutrient availability. Adding vermicompost leachate (VCL) improves the co-composting of organic waste and the biodegradation of organic compounds. This study demonstrates that food waste provides nitrogen and phosphorus for lettuce growth, and VCL addition increases nutrient levels in bioponics.

JOURNAL OF ENVIRONMENTAL MANAGEMENT (2023)

Review Engineering, Environmental

Untapped Potential: Applying Microbubble and Nanobubble Technology in Water and Wastewater Treatment and Ecological Restoration

Sining Zhou, Kyle Rafael Marcelino, Sumeth Wongkiew, Lianpeng Sun, Wuzhen Guo, Samir Kumar Khanal, Hui Lu

Summary: This review provides a systematic discussion on the fundamentals, applications, and challenges of microbubbles and nanobubbles technology.

ACS ES&T ENGINEERING (2022)

Article Agricultural Engineering

Carbamazepine facilitated horizontal transfer of antibiotic resistance genes by enhancing microbial communication and aggregation

Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang

Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.

BIORESOURCE TECHNOLOGY (2024)

Review Agricultural Engineering

A review of microbial responses to biochar addition in anaerobic digestion system: Community, cellular and genetic level findings

Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei

Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.

BIORESOURCE TECHNOLOGY (2024)

Review Agricultural Engineering

Advances in nitrogen removal and recovery technologies from reject water: Economic and environmental perspectives

Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba

Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Layered double hydroxide loaded pinecone biochar as adsorbent for heavy metals and phosphate ion removal from water

Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee

Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Machine learning-based prediction of methane production from lignocellulosic wastes

Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen

Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Engineering of Yarrowia lipolytica as a platform strain for producing adipic acid from renewable resource

Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek

Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Synergies of pH-induced calcium phosphate precipitation and magnetic separation for energy-efficient harvesting of freshwater microalgae

Sefkan Kendir, Matthias Franzreb

Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Solvothermal liquefaction of orange peels into biocrude: An experimental investigation of biocrude yield and energy compositional dependency on process variables

Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele

Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Enhancing nitrogen removal performance through intermittent aeration in continuous plug-flow anaerobic/aerobic/anoxic process treating low-strength municipal sewage

Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng

Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Mechanism of magnetite-assisted aerobic composting on the nitrogen cycle in pig manure

Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan

Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.

BIORESOURCE TECHNOLOGY (2024)

Review Agricultural Engineering

Recent advances in microalgal production, harvesting, prediction, optimization, and control strategies

Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal

Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Efficient supply with carbon dioxide from flue gas during large scale production of microalgae: A novel approach for bioenergy facades

Martin Kerner, Thorsten Wolff, Torsten Brinkmann

Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Carbon dioxide and methane as carbon source for the production of polyhydroxyalkanoates and concomitant carbon fixation

Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang

Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Contribution of zeolite to nitrogen retention in chicken manure and straw compost: Reduction of NH3 and N2O emissions and increase of nitrate

Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang

Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Exploring advanced phycoremediation strategies for resource recovery from secondary wastewater using a large scale photobioreactor

Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen

Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.

BIORESOURCE TECHNOLOGY (2024)