Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 413, Issue 21, Pages 5259-5266Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03502-5
Keywords
Hexagonal carbon-nitrogen tubes; Ag2S nanocrystals; Photoelectrochemical; Epithelial cell adhesion molecule; Magnetic separation
Funding
- Hunan Provincial Science and Technology Plan Project, China [2019TP1001]
- Innovation-Driven Project of Central South University [2020CX002]
Ask authors/readers for more resources
In this study, a photoelectrochemical assay based on hexagonal carbon-nitrogen tubes (HCNT) was developed for detecting circulating tumor cells. The assay shows good selectivity for MCF-7 cells, with a low detection limit, and was successfully applied for detection in human whole blood, indicating potential for clinical use.
In this work, we developed a photoelectrochemical assay for circulating tumor cells (CTCs) detection based on hexagonal carbon-nitrogen tubes (HCNT) as visible light-sensitive materials. The MCF-7 cell was selected as the model CTC and was captured through specific recognition between epithelial cell adhesion molecules (EpCAM) on the cell surface and anti-EpCAM antibodies. Anti-EpCAM antibody-modified magnetic nanoparticles were used to enrich and separate MCF-7 cells from samples. The detection signal was amplified by Ag2S nanocrystals, which can compete with HCNTs for absorbing visible light, leading to a decrease ofphotocurrent intensity. The linear range of the assay for MCF-7 cells is from 10 to 5000 cells mL(-1) , with a detection limit of 3 cells mL(-1) (S/D = 3). The assay has good selectivity for MCF-7 detection over HeLa cells. The assay was successfully applied for the detection of MCF-7 in human whole blood, which indicates the potential for clinical application.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available