Article
Chemistry, Analytical
Fangfang Wang, Yi Zhang, Desheng Chen, Zhuoqi Zhang, Zhengping Li
Summary: A sensitive Single Microbead (MB)-based Aptasensor coupled with Terminal deoxynucleotidyl transferase (TdT) triggered signal amplification (SMAT) was developed for detecting tumor-specific exosomal proteins. This method successfully detected exosomal EpCAM protein (Exo-EpCAM) from samples containing as low as 55 particles/µL tumor-derived exosomes, showing great potential for future clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Physical
Xiaoyun Lu, Jinlong Li, Congyu Li, Qianqian Lou, Kai Peng, Bijun Cai, Ying Liu, Yonghong Yao, Lina Lu, Zhenyang Tian, Hongwu Ma, Wen Wang, Jian Cheng, Xiaoxian Guo, Huifeng Jiang, Yanhe Ma
Summary: By combining evolutionary analysis, mutagenesis scanning, and rational design, we have engineered ZaTdT, an enzyme that shows greatly improved catalytic activity for 3'-ONH2-modified nucleotides, enabling highly efficient enzymatic DNA synthesis.
Review
Biochemistry & Molecular Biology
Jon Ashley, Indiia G. Potts, Femi J. Olorunniji
Summary: The use of polymerase enzymes in biotechnology has provided unprecedented control over DNA manipulation, with terminal deoxynucleotidyl transferase (TdT) being one of the most intriguing enzymes. TdT is capable of template independent stepwise addition of nucleotides onto an oligonucleotide chain. This review comprehensively discusses the unique properties and applications of TdT as a biotechnology tool, and explores its increasing role in various fields.
Article
Chemistry, Analytical
Xiaojun Wang, Jingru Yang, Yiming Xie, Guosong Lai
Summary: This study develops a novel colorimetric biosensing method for kanamycin antibiotic detection by utilizing a target biorecognition reaction to induce the self-assembly of G-quadruplexes and the aggregation of gold nanoparticles (Au NPs). The method has a wide linear range and ultrahigh sensitivity, and shows satisfactory results in dose-recovery experiments with real samples.
Article
Biochemistry & Molecular Biology
Jon Ashley, Anna-Lisa Schaap-Johansen, Mohsen Mohammadniaei, Maryam Naseri, Paolo Marcatili, Marta Prado, Yi Sun
Summary: The TdT enzyme plays a crucial role in V(D)J recombination in lymphocytes, allowing for diverse expression of immunoglobulins and T-cell receptors. A new screening method was demonstrated using TdT to form vsDNA libraries for binding to protein targets, offering the possibility of rational aptamer design and controlled reaction conditions for improved screening outcomes. This approach allows for screening based on both size and sequence in a single round, reducing PCR bias and resulting in high affinity and selective aptamers.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Analytical
Xinxin Wang, Jianguo Xu, Panzhu Qin, Chao Yan, Guodong Liu, Wei Chen
Summary: The isothermal method described utilizes PTA-MBs to assemble DNA nanostructures and achieve signal amplification for quantification of TdT activity, demonstrating excellent selectivity and practical applicability in direct/absolute quantification in real biological samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Zi-Tao Zhong, Yan-Fei He, Yuan-Ju Tang, Ghazala Ashraf, Huai Yang, Wei Chen, Bo Liu, Guo-Ping Wang, Yuan-Di Zhao
Summary: In this study, a rapid detection method for contaminant residue analysis in milk was developed using pregnancy test strips, providing multiple analytes detection with good accuracy and practicability.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Applied
Yangyang Zhou, Huiyuan Wang, Zhihui Zhao, Donglei Luan, Xiaojun Bian, Keqiang Lai, Juan Yan
Summary: We developed a colloidal SERS method using petal-like plasmonic nanoparticle (PLNP) clusters for the detection of enrofloxacin (EnFX). PLNPs were synthesized using single-stranded DNA composed of homo-cytosine deoxynucleotides (hC) catalyzed by terminal deoxynucleotidyl transferase. SERS hot spots were created by agglomeration of PLNPs using potassium iodide solution, allowing attachment of EnFX molecules to the negatively charged PLNPs surface via electrostatic interactions. This method enabled direct in situ detection of antibiotic residues with a limit of detection (LOD) of 1.15 µg/kg for EnFX. The spiked recoveries of the SERS method ranged from approximately 92.7% to 107.2% with RSDs ranging from 1.05% to 7.8%, indicating its applicability for actual sample detection. This colloidal SERS measurement platform shows great promise in various applications, especially for real-time and on-site food safety screening due to its rapidness, simplicity, and sensitivity.
Article
Chemistry, Analytical
Shengyong Ding, Ailing Kan, Xiaoting Liu, Chao Huang, Nan Zhang, Wei Jiang
Summary: A strand-elongation initiated DNAzyme walker has been developed to detect TdT activity, providing a specific and sensitive method for research and diagnosis related to leukemia.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Xinmei Song, Yujie Liu, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: Using a new phosphorothioate-modified DNA hairpin probe, we developed a label-free and highly sensitive electrochemical sensor for detecting abnormal myeloperoxidase (MPO) expression associated with inflammation and neurodegenerative diseases. This sensor combines DNAzyme-assisted cleavage recycling with DNA polymerization reaction to monitor MPO. It exhibits a linear range of 0.1-100 ng mL-1 and achieves a low detection limit of 0.046 ng mL-1 for MPO. The sensor is also highly selective against interferents and capable of detecting MPO in diluted human serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Spectroscopy
Zhimei Li, Ting Pi, Kefang Yang, Ziyi Xia, Yuchuan Feng, Xiangjuan Zheng, Ruihong Deng, Baozhu Chi
Summary: A label-free fluorescence probe based on poly-thymine copper nanoclusters engineered by terminal deoxynucleotidyl transferase is proposed for methyltransferase activity assay. This economical method demonstrates specificity and high efficiency for screening DNA methylation inhibitors, showing great potential for future drug development and clinical cancer diagnosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Multidisciplinary
Jiantong Dong, Itamar Willner
Summary: Native G-quadruplex-regulated temporal biocatalytic circuits, gene polymerization, and transcription processes are emulated by biomimetic, synthetically engineered transcription machineries coupled to reconfigurable G-quadruplex nanostructures. These systems introduce potential therapeutic applications and advance the field of dynamically modulated G-quadruplex DNA nanostructures.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Yue Sun, Liu Zang, Jianzhong Lu
Summary: In this study, a sensitive method was developed to simultaneously detect multiple DNA glycosylases. The method is based on the target-initiated removal of damaged base and terminal deoxynucleotidyl transferase (TdT)-assisted labeling and signal amplification. The method has the advantages of high sensitivity, specificity, and multiplex and can be used for DNA glycosylase-related biomedical research.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Jielin Chen, Mingpan Cheng, Jiawei Wang, Dehui Qiu, David Monchaud, Jean-Louis Mergny, Huangxian Ju, Jun Zhou
Summary: The structural integrity of the 3'-end G-quartet is necessary for G4s to be catalytically competent, and G-vacancy-mediated catalytic activity losses can be remediated via the G-vacancy complementation strategy. Furthermore, the terminal G-quartet can act as a proximal coordinating group and cooperate with the flanking nucleotide to activate the hemin cofactor.
CHINESE JOURNAL OF CATALYSIS
(2021)
Article
Pharmacology & Pharmacy
Weicong Ye, Longjie Li, Zishan Feng, Bocheng Tu, Zhe Hu, Xianjin Xiao, Tongbo Wu
Summary: A highly sensitive and selective method based on TdT and Endo IV was developed to quantify ALP activity. This method can detect the presence of ALP by detecting the elongation of a poly A tail, and has potential applications.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)