Review
Polymer Science
Kun Feng, Lulu Huangfu, Chuanduo Liu, Laura Bonfili, Qisen Xiang, Hong Wu, Yanhong Bai
Summary: Electrospinning and electrospraying are two electrohydrodynamic techniques that have attracted increased interest for stabilizing probiotics. They help improve the viability of probiotics and enhance their delivery in the gastrointestinal tract. This review discusses the classification of electrospinning and electrospraying, their feasibility in constructing probiotic carriers, and their efficacy in stabilizing and delivering probiotics. The current application of electrospun and electrosprayed probiotic formulations is also introduced. Finally, the limitations and future opportunities for using electrohydrodynamic techniques in probiotic stabilization are proposed and analyzed. This work provides a comprehensive understanding of how electrospinning and electrospraying can be used to enhance probiotics, aiding in the development of probiotic therapy and nutrition.
Review
Pharmacology & Pharmacy
Faiqa Nazir, Tanveer A. Tabish, Fatima Tariq, Shanza Iftikhar, Rijja Wasim, Gul Shahnaz
Summary: This review summarizes recent developments in stimuli-based targeted drug delivery systems for the site-specific release of antibiotics. By utilizing various stimuli, controlled and timely release of antibiotics at the site of infection can be achieved.
DRUG DISCOVERY TODAY
(2022)
Review
Biochemistry & Molecular Biology
Nirmeen Elmadany, Obada T. Alhalabi, Michael Platten, Lukas Bunse
Summary: This review summarizes different administration routes for CAR-T cell therapy in glioma, including local therapy, systemic administration, and intra-arterial drug delivery.
Review
Polymer Science
Maria Celina Sanchez-Cervino, Codrin Paul Fuioaga, Leonard Ionut Atanase, Gustavo A. A. Abraham, Guadalupe Rivero
Summary: The development of accurate drug delivery systems is a major challenge in the biomedical field. Various structures have been proposed as carriers for bioactive agents, with the goals of precision in administration and dosage, safety, and bioavailability. This review discusses the use of electrohydrodynamic techniques for the immobilization and synthesis of vesicles in a non-conventional way. The state of the art, advantages and limitations of electrospun and electrosprayed amphiphilic structures as precursor templates, and the perspectives and challenges of combined strategies for advanced drug delivery systems are analyzed.
Review
Biotechnology & Applied Microbiology
Haikun Liu, Geoffrey Pietersz, Karlheinz Peter, Xiaowei Wang
Summary: This article discusses the pathological mechanism of atherosclerosis and atherothrombosis, and the latest advancements in targeted therapy using drug delivery and nanoparticle carrier systems. It also explores the limitations and future perspectives of this treatment approach.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Polymer Science
Mallesh Kurakula, N. Raghavendra Naveen
Summary: Chitosan has been effectively utilized in biomedical applications, particularly in drug delivery, through the emerging technology of electrospraying. This technique enables successful loading and delivery of various therapeutic agents, and the review also discusses parameters, polymer types, advantages, and limitations associated with electrosprayed chitosan. The comprehensive analysis covers a range of biomedical applications and trends in patents related to electrosprayed chitosan products, highlighting the potential for commercialization and safe fabrication.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Tin Marsic, Sivakrishna Rao Gundra, Qiaochu Wang, Rashid Aman, Ahmed Mahas, Magdy M. Mahfouz
Summary: Programmable site-specific nucleases have a wide range of applications in basic biology research, biotechnology, and gene therapy. Using peptide nucleic acids (PNAs), we have developed a technology called PNA-assisted pAgo editing (PNP editing) that enables prokaryotic Argonaute proteins to introduce site-specific double-strand breaks (DSBs) independent of the target sequence. This technology has the potential for in vitro and in vivo applications, including genome editing.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Anabela Moreira, Dan Lawson, Lesley Onyekuru, Karolina Dziemidowicz, Ukrit Angkawinitwong, Pedro F. Costa, Norbert Radacsi, Gareth R. Williams
Summary: This review explores the potential of electrohydrodynamic (EHD) techniques in developing peptide and protein delivery systems, discussing the principles of various EHD methods and encapsulation of biological molecules using these approaches, as well as exploring the possibilities of localized delivery with stimuli-responsive systems and summarizing the advantages and challenges of electrospun and electrosprayed protein delivery systems.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Yunqi Yang, Qinyi Lu, Chao-Min Huang, Hongji Qian, Yunlong Zhang, Sonal Deshpande, Gaurav Arya, Yonggang Ke, Stefan Zauscher
Summary: The combination of SI-TcEP with DONs allows for the generation of stable, polynucleotide brush-functionalized DNA nanostructures with precise control over brush location, height, and composition. This results in increased nuclease resistance and spatially programmable stability. Site-specific decoration of DONs with brushes of natural and non-natural nucleotides provides access to a wide range of functionalized DON architectures for potential applications in smart nanoscale delivery systems.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Engineering, Biomedical
Niranjan G. Kotla, Rajbir Singh, Becca Baby, Swetha Rasala, Jawad Rasool, Sean O. Hynes, Darrell Martin, Laurence J. Egan, Praveen K. Vemula, Venkatakrishna R. Jala, Yury Rochev, Abhay Pandit
Summary: Delivering drugs directly to inflamed intestinal sites to treat inflammatory bowel disease (IBD) is a challenge, but the development of targeted carrier systems such as IT-NCs shows promising therapeutic potential in improving treatment efficacy.
Article
Materials Science, Biomaterials
Mei Yang, Chunhua Yang, Yujie Zhang, Xiangji Yan, Yana Ma, Yuanyuan Zhang, Yameng Cao, Qiuran Xu, Kangsheng Tu, Mingzhen Zhang
Summary: Ulcerative colitis (UC) is characterized by colonic epithelial barrier dysfunction and gut dysbiosis. Combination therapy of CD98 siRNA and berberine shows promising synergistic effects in alleviating inflammation and microbial dysbiosis in UC. The study demonstrates a precision nanotechnology-based strategy with enhanced bioavailability of siRNA for UC treatment.
BIOMATERIALS SCIENCE
(2022)
Article
Biology
Casandra Panea, Ruoyu Zhang, Jeffrey VanValkenburgh, Min Ni, Christina Adler, Yi Wei, Francisca Ochoa, Jennifer Schmahl, Yajun Tang, Chia-Jen Siao, William Poueymirou, Jennifer Espert, Wei Keat Lim, Gurinder S. Atwal, Andrew J. Murphy, Matthew A. Sleeman, Zaruhi Hovhannisyan, Sokol Haxhinasto
Summary: Panea et al. demonstrated that Btnl2 plays a crucial role in maintaining homeostasis of gamma delta IELs in the ileum by suppressing their proliferation. Btnl2 deficiency affects TCR specificities and diversity of ileal gamma delta IELs, leading to increased inflammation and delayed mucosal repair in the colon during DSS-induced colitis, highlighting the immunological importance of Btnl2 in intestinal diseases.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Marco A. Arriaga, Juan A. A. Amieva Jr, Jaqueline Quintanilla, Angela Jimenez, Julio Ledezma, Silverio Lopez, Karen S. Martirosyan, Sue Anne Chew
Summary: This study aimed to fabricate minocycline-loaded PLGA microparticles with high drug loading and loading efficiency using the electrospraying process, and to evaluate their cytotoxicity in human glioblastoma U87-MG cells. The results showed that the solvent mixture of DCM and methanol (70:30) was the optimal solvent combination for minocycline, producing spherical and smooth microparticles in the micron size range. The electrosprayed microparticles demonstrated a cytotoxic response in U87-MG glioblastoma cells at a lower drug concentration. This work suggests that electrosprayed minocycline-loaded PLGA microparticles may hold promise for the treatment of GBM and other cancer therapies.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Ahmed I. Foudah, Mohammed H. Alqarni, Samir A. Ross, Aftab Alam, Mohammad Ayman Salkini, Piyush Kumar
Summary: This study provides a comprehensive evaluation of dendrimer G4 poloxamer nanoparticles loaded with bioactive coumarin against MRSA. The results showed that these newly developed nanoparticles have lower levels of hemolysis and cytotoxicity, and coumarin released from the nanoparticles at a slower rate, potentially enhancing its defense against MRSA.
Article
Chemistry, Multidisciplinary
Meng Xiao, Shuyang Wu, Yanfen Cheng, Jiaqi Ma, Xi Luo, Liang Chang, Chen Zhang, Jianping Chen, Liang Zou, Yu You, Jinming Zhang
Summary: In this study, an oral colon-specific nano-system composed of natural compounds and edible materials was developed for the prevention and treatment of ulcerative colitis. The optimized nanoparticles showed high drug loading efficiency and stability, and demonstrated effectiveness in both in vitro and in vivo experiments for the treatment of ulcerative colitis.
FRONTIERS IN CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Jingjing Li, Feng Li, Fan Tang, Jinming Zhang, Renkai Li, Dekuan Sheng, Simon Ming-Yuen Lee, Guo-Chun Zhou, George Pak-Heng Leung
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jingjing Li, Fan Tang, Renkai Li, Zhejie Chen, Simon Ming-Yuen Lee, Chaomei Fu, Jinming Zhang, George Pak-Heng Leung
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2020)
Article
Pharmacology & Pharmacy
Yaoyao Luo, Jingjing Li, Yichen Hu, Fei Gao, George Pak-Heng Leung, Funeng Geng, Chaomei Fu, Jinming Zhang
ACTA PHARMACEUTICA SINICA B
(2020)
Article
Medicine, Research & Experimental
Phoebe C. I. Ng, Judy Y. W. Chan, Ross K. K. Leung, J. Li, Z. Ren, Anthony W. H. Chan, Y. Xu, S. S. Lee, R. Wang, Xia Ji, Jun Zheng, Denise P. C. Chan, W. Yew, Simon M. Y. Lee
BIOMEDICINE & PHARMACOTHERAPY
(2020)
Article
Plant Sciences
Jinfeng Shi, Jingjing Li, Jiaxin Li, Renkai Li, Xiaoping Wu, Fei Gao, Liang Zou, Winston Wing Shum Mak, Chaomei Fu, Jinming Zhang, George Pak-Heng Leung
Summary: The study optimized the drug ratio of glycyrrhetinic acid (GA) and doxorubicin (Dox) to enhance the anti-cancer effect against MCF-7 breast cancer cells, showing significant improvements in cytotoxicity, apoptosis, and mitochondrial membrane potential loss. The addition of GA increased Dox accumulation in cells and the combination inhibited HUVECs proliferation, migration, and tube formation through down-regulation of the VEGFR2-mediated pathway, indicating a promising synergistic anti-angiogenesis effect. In a mouse breast tumor model, the combination of Dox with GA demonstrated enhanced anti-tumor efficacy and reduced cardiotoxicity from Dox.
Article
Cell Biology
Jingjing Li, Renkai Li, Xiaoping Wu, Ruby Lai-Chong Hoo, Simon Ming-Yuen Lee, Timothy Man-Yau Cheung, Bryan Siu-Yin Ho, George Pak-Heng Leung
Summary: The study found that the extract of Amauroderma rugosum possesses antioxidant and neuroprotective effects, reducing the toxicity induced by 6-hydroxydopamine in PC12 cells, possibly through influencing multiple signaling pathways.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Pharmacology & Pharmacy
Emily Sze-Wan Wong, Rachel Wai-Sum Li, Jingjing Li, Renkai Li, Sai-Wang Seto, Simon Ming-Yuen Lee, George Pak-Heng Leung
Summary: Narirutin induces relaxation in rat mesenteric arteries through mechanisms involving phosphodiesterase inhibition, leading to increased intracellular cAMP, stimulation of endothelial nitric oxide synthase, and activation of voltage-gated potassium channels in vascular smooth muscle cells.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xiaoping Wu, Lingling Shu, Zixuan Zhang, Jingjing Li, Jiuyu Zong, Lai Yee Cheong, Dewei Ye, Karen S. L. Lam, Erfei Song, Cunchuan Wang, Aimin Xu, Ruby L. C. Hoo
Summary: Liver fibrosis results in significant changes in the liver microenvironment, which promotes disease progression. The study demonstrates that increased expression of adipocyte fatty acid binding protein (A-FABP) in liver sinusoidal endothelial cells (LSECs) plays a crucial role in promoting liver fibrosis in mice. A-FABP activates signaling pathways that lead to liver fibrosis progression, suggesting that targeting A-FABP may be a potential therapeutic approach against liver fibrosis.
Article
Biotechnology & Applied Microbiology
Xiao Wang, Shu Fu, Chen Zhang, Xin Nie, Wan Liao, Ming Zhao, Fang Liu
Summary: The ethanol extracts from the bark of Magnolia officinalis (MOE) demonstrated strong antioxidant and gastroprotective effects against ethanol-induced gastric mucosal damage in rats. The mechanism of action involves inhibiting oxidative stress and the NF-KB inflammatory pathway, suggesting the potential of MOE as a promising phytomedicine for gastric ulcer prevention.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Qian Liao, Lan Pang, Jing-Jing Li, Chen Zhang, Jia-Xing Li, Xing Zhang, Ting Mao, Ding-Tao Wu, Xiu-Ying Ma, Fu-Neng Geng, Jin-Ming Zhang
Summary: This study investigated the bioactive wound healing substances in Periplaneta americana L. and found that its protein-polysaccharide complexes have the potential to promote cell proliferation, migration, and angiogenesis, thereby accelerating wound healing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Zhuyun Liu, Xiaoping Wu, Kun Dai, Renkai Li, Jinming Zhang, Dekuan Sheng, Simon Ming-Yuen Lee, George Pak-Heng Leung, Guo-Chun Zhou, Jingjing Li
Summary: The anti-cancer effect of AGS-30 is stronger than that of Andro, inducing apoptosis of colon cancer cells through ROS/JNK-dependent pathway. These findings may provide insights for the future development of derivatives of Andro as novel chemotherapeutic agents.
Article
Pharmacology & Pharmacy
Jing Xian, Xuemei Zhong, Huan Gu, Xiao Wang, Jiaxin Li, Jingjing Li, Yihan Wu, Chen Zhang, Jinming Zhang
Summary: A flexible oral colon-targeting delivery system mediated by pectin-trimethyl chitosan coating was developed to enhance anti-UC efficacy. The system, with a bilayer coating of polysaccharides, reduced drug cytotoxicity, improved mucosal adhesion, and prolonged drug retention in the colon. Ultimately, this system significantly alleviated colitis symptoms and accelerated colitis repair in mice by regulating inflammation-related factors.
Article
Medicine, Research & Experimental
Liang Zou, Xiaowei Liu, Jingjing Li, Wei Li, Lele Zhang, Chaomei Fu, Jinming Zhang, Zhongwei Gu
Summary: This study developed a redox-sensitive nanosystem for delivering paclitaxel and tetramethylpyrazine simultaneously, demonstrating excellent anticancer effects and anti-angiogenic activity in vivo. The amphiphilic drug conjugate self-assembled into stable nanoparticles with high drug loading, and showed improved therapeutic outcomes compared to monotherapy or combination therapy with free drugs.