Article
Chemistry, Analytical
Xianxian Zhao, Ye Yuan, Xiaoya Liu, Fajiang Mao, Ge Xu, Qingzhong Liu
Summary: The development of a versatile and sensitive analytical biomarker detection platform is crucial for early disease diagnosis and treatment. In this study, a novel fluorescence-based, ultrasensitive, and label-free biomarker detection platform is proposed. This platform demonstrates high sensitivity and specificity, and shows great potential in clinical diagnosis and anticancer drug development.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Zhaorui Song, Yongkuan Suo, Shuang Duan, Shanshan Zhang, Lifu Liu, Botong Chen, Zhen Cheng
Summary: Fluorescent lateral flow immunoassay (LFA) based on rare earth doped nanoparticles (RENPs) emitting in the second near-infrared (NIR-II) window is a common analytical platform for point-of-care testing (POCT) that enables easy and early screening of biomarkers. This platform shows high sensitivity, low cost, and has potential for early diagnosis of tumor biomarkers.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Multidisciplinary
Siew Suan Ng, Hooi Ling Lee, Pandian Bothi Raja, Ruey-an Doong
Summary: This review focuses on the application of optical biosensors for carcinoembryonic antigen (CEA) detection. Many nanomaterial-based biosensors have been developed as alternatives to traditional methods, but they have certain drawbacks. The advantages of optical biosensors, such as sensitivity and being free from interference, are discussed. The roles of nanomaterials in each application are summarized, and the challenges and future prospects are highlighted.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Article
Chemistry, Analytical
Yi Gu, Yang Guo, Yang Deng, Haipeng Song, Rui Nian, Wenshuai Liu
Summary: A novel pentameric nanobodies-based immunoassay (PNIA) demonstrated superior sensitivity and high specificity for detecting CEACAM-5 antigen. The use of bispecific or multivalent nanobody design provided new insights into the development of immunoassays for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Chaoqun Wang, Ziqiang Deng, Hu Zhang, Rui Liu, Yi Lv
Summary: Magnetic particles (MPs) are widely used in various biological applications, and this study discovered a novel function of commercialized MPs-internal standard for accurate quantification of biomolecules. The proposed MPs-based immunoassay greatly improves the accuracy and precision by eliminating potential MPs loss and intensity fluctuations during magnetic separation.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Analytical
Yingzhou Tao, Weihao Shi, Fang Luo, Bin Qiu, Zhenyu Lin
Summary: Gold nanoparticles exhibit strong photothermal effect after aggregation induced by cysteine and are inhibited by copper ions, enabling detection of Cu2+ and development of a convenient photothermal immunoassay for cancer biomarker CEA.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Xuwei Chen, Xuan Zheng, Xiangyong Yu, Xiaoqin Li, Youxiu Lin, Huizi Lin, Shuai Ye, Xinyu Huang, Dianping Tang, Wenqiang Lai
Summary: This study reports a simple and rapid colorimetric immunoassay for the determination of carcinoembryonic antigen (CEA) using Fe2O3 nanoparticle-based chromogenic substrate system. The assay is based on the rapid coordination of ascorbic acid 2-phosphate (AAP) and iron (III), resulting in color development. Fe2O3 nanoparticle is used as a label in a sandwich-type immunoassay, and the absorbance of the reaction solution is positively correlated with antigen concentration. The method shows good performance for CEA detection, with a wide detection range and a low detection limit, as well as acceptable repeatability, stability, and selectivity.
Article
Materials Science, Biomaterials
Nanhang Zhu, Guohao Li, Juan Zhou, Yujia Zhang, Ke Kang, Binwu Ying, Qiangying Yi, Yao Wu
Summary: In this study, a FRET magnetic aptamer-sensor was developed for rapid and efficient detection of exosomes, with a detection limit of 13 particles per mL and 100% accuracy in screening a specific cancer antigen. This system shows promising potential for liquid biopsy in lung cancer detection.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Analytical
Xianlin Han, Shujin Lin, Cui Cheng, Xiao Han, Dianping Tang
Summary: This study presents an integrated photoelectrochemical, impedance and colorimetric biosensing platform for flexible detection of cancer markers by combining liposome amplification strategies and target-induced non-in situ formation of electronic barriers as the signal transduction modality. The platform enables high sensitivity detection through the amplification strategy of liposome-mediated enzymatic reaction.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Tohid Mahmoudi, Mohammad Pourhassan-Moghaddam, Behnaz Shirdel, Behzad Baradaran, Eden Morales-Narvaez, Hamed Golmohammadi
Summary: A convenient kit was developed for on-site detection of CEA in human serum, utilizing cellulose-based lateral flow strips and a smartphone-based system for signal reading and processing. Excellent correlation was demonstrated in clinical sample testing, showing potential for monitoring cancer treatment.
Article
Chemistry, Analytical
Ruina Wang, Yun Huang, Yuwu Chi
Summary: An ECL immunosensor for the detection of carcinoembryonic antigen (CEA) was constructed using oxidized multi-walled carbon nanotube/nano-gold (AuNP-ox-MWCNT) composites with strong electrochemiluminescence (ECL) activity. The immunosensor showed a wide linear response range and a low detection limit, and was simple to fabricate and label-free.
Article
Chemistry, Analytical
Asanka Gurukandure, Subramaniam Somasundaram, Amanda S. N. Kurian, Niamat Khuda, Christopher J. Easley
Summary: This study reports a new nucleic acid nanostructure sensor that allows for the general detection of larger proteins by conjugating the protein's antibody-binding epitope to the DNA strand. The sensor was validated for the quantification of creatine kinase, a clinically relevant protein, through competitive immunoassay and signal-on mechanism. However, further improvement in the purification of DNA-epitope conjugates is needed.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Haiyang Wang, Xinyu Wan, Xin Wang, Meijin Li, Dianping Tang
Summary: In this study, a liposome-amplification photoelectrochemical (PEC) immunoassay was developed using ultrathin mesoporous bismuth chloride oxide nanosheets (BiOCl MSCN) for the highly selective and sensitive detection of carcinoembryonic antigen (CEA).
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Xiaoting Xu, Xuan Li, Juncong Miao, Lei Liu, Xinyi Huang, Qin Wei, Wei Cao
Summary: A label-free electrochemical immunosensor based on DPV and i-t dual-mode analysis was developed for early quantitative detection of PCT. Using materials with high stability and biocompatibility as sensing interface, the sensor showed wide linear response and low detection limit through an advanced sensing strategy.
Article
Chemistry, Analytical
Xue Huang, Qianyun Lin, Liling Lu, Meijin Li, Dianping Tang
Summary: This study reported a photoelectrochemical immunoassay for the detection of carcinoembryonic antigen (CEA) using In2O3/CdIn2S4 heterojunctions as the photosensitizers. The heterojunctions improved charge separation efficiency and photocurrent, and the target-induced biocatalytic precipitation provided signal amplification. The method showed excellent detection performance for CEA with low limit of detection, and demonstrated reliability and stability.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Xiwen Jiang, Cuiyuan Pan, Qiaowen Wang, Xiao Han, Dianping Tang
Summary: An integrated photoelectrochemical (PEC) immunosensing platform was designed for the quantitative monitoring of thyroglobulin (TG). This system simultaneously achieved the immunoreaction and photocurrent measurement, with good repeatability and precision, high specificity and acceptable storage stability. No significant differences were encountered for screening human serum specimens between the developed PEC immunoassay and commercially available enzyme-linked immunosorbent assay (ELISA) method.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Min Wei, Jingwen Bai, Xiao Shen, Kangliang Lou, Yiyang Gao, Ruichan Lv, Peiyuan Wang, Xiaolong Liu, Guojun Zhang
Summary: A nanoprobe is designed to address the challenges in accurate margin assessments and enhance radiotherapy in breast cancer. The nanoprobe targets tumor cells efficiently, allows for precise surgery under NIR-II fluorescence imaging, and boosts radiation sensitivity by disrupting intracellular ROS homeostasis.
Article
Biophysics
Liling Lu, Xuehan Hu, Ruijin Zeng, Qianyun Lin, Xue Huang, Qiaohua Wei, Dianping Tang, Dietmar Knopp
Summary: In this study, a dual-readout gasochromic immunosensing platform based on Ag-doped/Pd nanoparticles loaded MoO3 nanorods (Ag/MoO3-Pd) was presented for accurate and sensitive detection of carcinoembryonic antigen (CEA). The platform utilizes the interaction of hydrogen gas (H2) with Ag/MoO3-Pd as a bridge between the sensing interface and the biological assembly platform. Both photocurrent and temperature signals can be used as readouts due to the improved performance of H-Ag/MoO3-Pd compared to Ag/MoO3-Pd. The designed immunosensing platform showed good sensitivity for CEA detection with low limits of detection in both photoelectrochemical and photothermal modes.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Xuwei Chen, Xuan Zheng, Xiangyong Yu, Xiaoqin Li, Youxiu Lin, Huizi Lin, Shuai Ye, Xinyu Huang, Dianping Tang, Wenqiang Lai
Summary: This study reports a simple and rapid colorimetric immunoassay for the determination of carcinoembryonic antigen (CEA) using Fe2O3 nanoparticle-based chromogenic substrate system. The assay is based on the rapid coordination of ascorbic acid 2-phosphate (AAP) and iron (III), resulting in color development. Fe2O3 nanoparticle is used as a label in a sandwich-type immunoassay, and the absorbance of the reaction solution is positively correlated with antigen concentration. The method shows good performance for CEA detection, with a wide detection range and a low detection limit, as well as acceptable repeatability, stability, and selectivity.
Article
Chemistry, Analytical
Liangjie Chi, Xiangyu Wang, Hongyuan Chen, Dianping Tang, Fangqin Xue
Summary: A portable photoelectrochemical split diagnostic platform based on a hollow CdS/CdMoO4 shell-shell structured photoanode system was developed for ultrasensitive detection of CEA. The system utilized a small LED flashlight as the excitation light source and a digital multimeter as the signal readout device, enabling quick detection of CEA on a paper-based printed screen electrode. The portable PEC sensing system showed high sensitivity to CEA with a detection limit of 11.3 pg mL-1.
Article
Chemistry, Multidisciplinary
Xinyi Lin, Feida Li, Jianhua Guan, Xiaoyan Wang, Cuiping Yao, Yongyi Zeng, Xiaolong Liu
Summary: An immunomodulator based on Janus silica nanoparticles is developed to reshape the tumor microenvironment and improve the efficacy of PD-1/PD-L1 immune checkpoint blockade therapy. This immunomodulator releases a PI3K? inhibitor to reverse immunosuppression in the tumor microenvironment. The results demonstrate that this strategy significantly improves immune suppression in the tumor microenvironment and induces robust systemic antitumor immune responses, enhancing the efficacy of PD-1/PD-L1 blockade therapy.
Article
Chemistry, Analytical
Xin Wang, Haiyang Wang, Xinyu Wan, Meijin Li, Dianping Tang
Summary: The study presents a portable photoelectrochemical (PEC) immunoassay platform for rapid and low-cost detection of carcinoembryonic antigen (CEA) using a smartphone. The system utilizes disposable screen-printed carbon electrodes and a S-scheme heterojunction BiOCl/CuBi2O4 to achieve efficient electron transfer and excellent photocurrent response. The proposed immunoassay demonstrates good linearity within the concentration range of 0.01-40 ng mL-1 and a detection limit of 3.5 pg mL-1 (S/N = 3).
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Xianlin Han, Shujin Lin, Cui Cheng, Xiao Han, Dianping Tang
Summary: This study presents an integrated photoelectrochemical, impedance and colorimetric biosensing platform for flexible detection of cancer markers by combining liposome amplification strategies and target-induced non-in situ formation of electronic barriers as the signal transduction modality. The platform enables high sensitivity detection through the amplification strategy of liposome-mediated enzymatic reaction.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Shuyun Chen, Zhichao Yu, Yunsen Wang, Juan Tang, Yongyi Zeng, Xiaolong Liu, Dianping Tang
Summary: This study successfully constructed stable platinum nanoclusters using an organic polymer surfactant and demonstrated high peroxidase-like activity, providing an enhanced method for the immunoassay of cardiac troponin I. This research is of great significance for designing artificial enzymes with high enzyme-like activity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Hang You, Ming Wang, Shuai Wang, Jie Xu, Shuhao Hu, Tianhua Li, Zhenzhong Yu, Dianping Tang, Ning Gan
Summary: This research developed an ultrasensitive and specific Phage@DNAzyme signal probe for the rapid and on-site detection of virulent foodborne pathogens. The probe efficiently catalyzed a click reaction to generate a visual fluorescent signal, allowing for the quantification of target concentrations using a smartphone. The method demonstrated satisfactory sensing performances for Escherichia coli O157:H7 and Salmonella typhimurium, providing a new approach for trace level detection of pathogenic strains in foods.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Liling Lu, Ruijin Zeng, Qianyun Lin, Xue Huang, Dianping Tang
Summary: In this study, the transformation of ZnIn2S4 after CE reaction with Cu2+ ions was explored, and a dual-readout detection system was designed for reliable detection of CEA. Based on the polarity-switchable PEC strategy, CEA could be accurately detected using both photothermal and photoelectrochemical signals.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Haiyang Wang, Xinyu Wan, Xin Wang, Meijin Li, Dianping Tang
Summary: In this study, a liposome-amplification photoelectrochemical (PEC) immunoassay was developed using ultrathin mesoporous bismuth chloride oxide nanosheets (BiOCl MSCN) for the highly selective and sensitive detection of carcinoembryonic antigen (CEA).
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Minghao Qiu, Yuqing Ren, Lumin Huang, Xueying Zhu, Tikai Liang, Meijin Li, Dianping Tang
Summary: A sensitive electrochemical immunoassay platform for quantitatively monitoring human epidermal growth factor receptor 2 (HER2) is developed using iron nitrogen-doped carbon (FeNC) nanozyme-modified screen-printed carbon electrode and chronoamperometry. The system demonstrates good amperometric response and can detect target HER2 as low as 4.5 pg/mL. FeNC nanozyme-based sensing strategy offers high specificity and long-term stability, providing a new opportunity for protein diagnostics.
Article
Chemistry, Analytical
Lixia Su, Bingqian Liu, Yonghuan Su, Dianping Tang
Summary: In this study, a dual-signal sensing platform for simultaneous readout of pressure and temperature was developed using NIR II light response-based PDA/AuPt@CuS NS composite. The platform utilized the photothermal performance of PDA/AuPt@CuS NS to amplify pressure signals in a sealed container. It also employed a sandwich mode between Fe3O4 NPs and PDA/AuPt@CuS NS for the detection of pathogenic bacteria and conversion of molecular recognition signals into amplified pressures and temperatures.