Article
Engineering, Chemical
Anna A. Ampaw, Kayla Newell, Robert N. Ben
Summary: In this study, sodium phosphonate compounds were synthesized to improve the solubility and activity of ice recrystallization inhibitors. Among them, two compounds showed IC50 values below 30mM. The addition of a polar sodium phosphonate group was found to decrease the IRI activity of alkyl gluconamide and aryl glucoside, highlighting the importance of hydrophobic/hydrophilic balance. Additionally, the study revealed the ability of ammonium to modulate the IRI activity of its paired anion.
Article
Nanoscience & Nanotechnology
Ranga Dissanayake, Diego Combita, Marya Ahmed
Summary: Development of new cryopreservation technologies has the potential to revolutionize various medical fields. Existing cryopreservation agents have limitations due to their cytotoxic and immunogenic nature, but nanodimensional cell impermeable agents show promise. This study reports the synthesis of nanogels incorporating a vitamin B5 analog, which demonstrated significant ice recrystallization inhibition and comparable or superior cryopreservation efficacy compared to traditional cryoprotectants.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Cell & Tissue Engineering
Salma Alasmar, Jez Huang, Karishma Chopra, Ewa Baumann, Amy Aylsworth, Melissa Hewitt, Jagdeep K. Sandhu, Joseph S. Tauskela, Robert N. Ben, Anna Jezierski
Summary: This study investigated the role of ice recrystallization inhibitors (IRIs) as cryoprotectants for iPSCs and iPSC-derived neurons (iPSC-Ns). The results showed that IRIs, specifically 2FA, increased the post-thaw viability and recovery of iPSCs without affecting pluripotency. Although 2FA supplementation did not significantly improve iPSC-N cell post-thaw viability, 2FA cryopreserved iPSC-Ns exhibited better restoration of neuronal network activity and synaptic function compared to controls. This study highlights the importance of optimizing cryopreservation media formulations with IRIs to improve functional cryopreservation of iPSCs and post-mitotic iPSC-Ns.
Article
Chemistry, Multidisciplinary
Zhang Liu, Huimei Cao, Yaxun Fan, Yilin Wang, Jianjun Wang
Summary: Spherical crowders, fibrous crowders, and coacervates were constructed to mimic three typical crowders in biological fluids. It was found that the three different crowders exhibit low, moderate, and strong ice growth inhibition activity, respectively, due to their different abilities in slowing down water dynamics. Intriguingly, the coacervate consisting of molecules without obvious ice growth inhibition activity strongly inhibits ice growth, making it a highly-potent cryoprotectant. This work provides new insights into the survival of freezing-tolerant organisms and opens a new avenue for the design of ice-controlling materials.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Zhongxiang Ding, Chao Wang, Baomei Zhou, Mengke Su, Shixuan Yang, Yuzhu Li, Cheng Qu, Honglin Liu
Summary: We report several new findings about ice recrystallization inhibition (IRI) and demonstrate for the first time that a dense hydroxyl monolayer of small molecules adsorbed on a nanogold surface has IRI activity. The IRI mechanism is closely related to the density of hydroxyls on the surface, and hydrophobic interaction is not essential for macroscopic IRI activity. Molecular dynamics simulation reveals the hydroxyl density dependent IRI trajectories, and the presence of methyl hinders the formation of hydrogen bonding due to a hydrophobic interaction.
Article
Pharmacology & Pharmacy
Anna Otterbach, Alf Lamprecht
Summary: Dimethyl sulfoxide is a widely used dermal penetration enhancer, but its volatility poses challenges in incorporating it into transdermal patches. Research shows that transdermal systems containing DMSO can enhance drug permeation through skin and inhibit drug recrystallization.
Article
Materials Science, Biomaterials
Bing Hu, Juan-Juan Li, Yan-Bin Ren, Tian-Xing Zhang, Li-Bin Chen, Xiao-Liu Li, Dong-Sheng Guo, Ke-Rang Wang
Summary: The development and study of five calixarene-based cryoprotectants in this research demonstrated their excellent cryoprotective effects, especially for those with specific functional groups. This provides a supramolecular principle for the development of novel cryoprotectants.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Engineering, Biomedical
Min Liu, Changhong Chen, Jiajun Yu, Haitao Zhang, Lei Liang, Bingyan Guo, Yuwei Qiu, Fanglian Yao, Hong Zhang, Junjie Li
Summary: Liquid marble, as an alternative cryopreservation tool, shows promising potential in protecting cells efficiently without the need for toxic additives like DMSO and FBS. Gelatin-based liquid marble exhibits superior biocompatibility and ice recrystallization inhibition activity, providing a safe and effective method for cell preservation.
MATERIALS TODAY BIO
(2022)
Article
Engineering, Chemical
Jing Yang, Min Liu, Tiantong Zhang, Jianfan Ma, Yiming Ma, Shu Tian, Ruiqi Li, You Han, Lei Zhang
Summary: In this study, different types of antifreeze organism-inspired ICMs were designed to regulate ice crystals and provide cell-friendly protection. This protection mechanism can effectively improve the efficiency of cryopreservation and further support cell-based therapies.
Review
Biochemistry & Molecular Biology
Xiaojun Wu, Fanglian Yao, Hong Zhang, Junjie Li
Summary: Cell-based therapy relies on cryopreservation technology for the production of therapeutic cells, with antifreeze proteins (AFPs) and their mimetics playing a crucial role in post-thaw cell viability. The current state of AFP mimetics fabrication is chaotic, lacking common guidelines for design. A comprehensive understanding of the antifreezing mechanism of AFPs will facilitate the reconstruction of their function and help clarify the relationship between structure and function in early stage biomimetics.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Trisha L. Bailey, Juan Ramon Hernandez-Fernaud, Matthew Gibson
Summary: Using proline as a protective osmolyte can enhance post-thaw cell yields and growth rate, along with the addition of ice recrystallization inhibitors leading to further increases in yield. The main mechanism of proline action is biochemical rather than biophysical, preparing the cells for cold stress. The results suggest that a chemical biology approach utilizing small molecule biochemical modulators of cryopreservation can be effective in improving cell monolayer cryopreservation.
RSC MEDICINAL CHEMISTRY
(2021)
Article
Biology
Audrey K. Gruneberg, Laurie A. Graham, Robert Eves, Prashant Agrawal, Richard D. Oleschuk, Peter L. Davies
Summary: Ice-binding proteins inhibit ice growth through surface adsorption, with different IBPs showing varying levels of ice recrystallization inhibition activity. Differences in IRI activity are seen between IBPs of different sizes and sources.
Article
Materials Science, Biomaterials
Min Liu, Changhong Chen, Lei Liang, Chaojie Yu, Bingyan Guo, Haitao Zhang, Yuwei Qiu, Hong Zhang, Fanglian Yao, Junjie Li
Summary: In this study, a DMSO analogue called l-methionine sulfoxide (Met(O)-OH) was investigated as a cryoprotectant for cell cryopreservation. The results showed that Met(O)-OH could stably bind the surface of ice crystals and form stable hydrogen bonds with ice. Additionally, Met(O)-OH significantly reduced cell damage caused by osmotic shock and had high post-thaw survival efficiency for L929 cells.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Oncology
Xue Yao, Sandro Matosevic
Summary: DMSO, commonly used as a cryoprotectant in clinical adoptive cell therapy, has broad toxicities and is associated with various clinical side effects in patients. Developing alternatives to DMSO for cryopreservation of NK and T cells remains challenging due to their sensitivity to freezing and thawing.
Article
Cell & Tissue Engineering
Renata Gilfanova, Kirsten M. Auclair, Alvin Hui, Philip J. Norris, Marcus O. Muench
Summary: The study investigated the cryopreservation of human bone marrow using reduced concentrations of DMSO and found that 5% DMSO was as effective as higher concentrations in preserving hematopoietic stem cells. Different methods of analyzing stem cell engraftment in mice showed consistent results in supporting the cryopreservation of HSCs with reduced concentrations of DMSO.
Article
Biochemistry & Molecular Biology
Heeju Kim, Yejin Woo, Madhumita Patel, Byeongmoon Jeong
Article
Chemistry, Physical
Hyeokjung Lee, Min Koo, Chanho Park, Madhumita Patel, Hyowon Han, Tae Hyun Park, Pawan Kumar, Won-Gun Koh, Cheolmin Park
Review
Polymer Science
Madhumita Patel, Amitabh Jha, Rajkumar Patel
Summary: Sustained release of drugs within a therapeutic range is effective for reducing patient discomfort and increasing compliance. PLGA microspheres, with their biocompatibility and sustained drug release properties, are ideal candidates for drug delivery in tissue regeneration, particularly in bone, cartilage, and neuron regeneration.
JOURNAL OF POLYMER RESEARCH
(2021)
Article
Nanoscience & Nanotechnology
Yejin Woo, Madhumita Patel, Hyelin Kim, Jin Kyung Park, Yeon-Ju Jung, Sun-Shin Cha, Byeongmoon Jeong
Summary: Folic acid and clinically approved antifolates were found to significantly improve chondrogenic differentiation of mesenchymal stem cells. A poly(ethylene glycol)-poly(L-alanine) thermogelling system was used to incorporate stem cells and antifolates for sustained release. Pralatrexate was discovered as an effective compound for promoting chondrogenic differentiation, with nuclear binding factor and transcription factor involvement. This study highlights the potential of using antifolates in tissue engineering for cartilage repair.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Chemistry, Applied
Madhumita Patel, Maninder Meenu, Jitender Kumar Pandey, Pawan Kumar, Rajkumar Patel
Summary: Ion channels in nerve cells are responsible for transmitting electric signals, and their malfunction can lead to neurological diseases. Optogenetic technology, which utilizes light to control neuronal functions, has gained attention. Lanthanide-doped upconversion nanoparticles (UCNPs) are a promising candidate for optogenetics due to their ability to absorb low energy photons and emit high energy photons. UCNPs have potential for deep tissue manipulation of ion channels. This article summarizes the photoluminescence and tuning mechanisms of UCNPs, explores their application in neural circuit modulation through optogenetics, and discusses future perspectives in this field.
JOURNAL OF RARE EARTHS
(2022)
Review
Chemistry, Applied
Zirui Zheng, Madhumita Patel, Rajkumar Patel
Summary: The complex healing process of bone and cartilage injuries has led to efforts to develop various bone tissue engineering techniques. Composite hydrogel systems, such as those using hyaluronic acid, have shown potential in promoting osteogenesis and supporting stem cell activities. This review discusses recent advancements in the development of hyaluronic acid hydrogel systems for in vitro and in vivo applications in bone tissue engineering.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Polymer Science
Zhengyu Piao, Jin Kyung Park, Madhumita Patel, Hyun Jung Lee, Byeongmoon Jeong
Summary: This study found that poly(L-alanine-co-L-lysine) (PAK) exhibits IRI activity similar to poly(vinyl alcohol) and generates cubic-to-spherical shaped ice crystals, showing superior performance. In contrast, poly(L-alanine-co-L-aspartic acid) (PAD) and poly(ethylene glycol) (PEG) with similar molecular weights did not provide significant IRI activity. FTIR and circular dichroism spectroscopies indicated that PAK forms alpha-helices, while PAD forms random coils in water. Additionally, dynamic ice shaping study suggested strong interaction between PAK and ice crystals, while PAD and PEG only weakly interact.
Review
Polymer Science
Se Min Kim, Madhumita Patel, Rajkumar Patel
Summary: Core-shell particles are well known for their unique structure and promising biomedical applications. PLGA, an FDA-approved polymer, is recognized as a vehicle material for drug delivery systems based on these particles. These systems are utilized for cancer therapy and tissue regeneration, particularly bone, cartilage, and periodontal regeneration.
Article
Biochemistry & Molecular Biology
Zhengyu Piao, Madhumita Patel, Jin Kyung Park, Byeongmoon Jeong
Summary: Poly(L-alanine-co-L-lysine)-graft-trehalose (PAK(T)) was synthesized as a cryoprotectant for mesenchymal stem cells (MSCs). The delivery of intracellular PEG-PAK(T) significantly improved cell recovery and maintained multilineage potential. The interaction between intracellular PEG-PAK(T) and water stabilized the cells during cryopreservation.
Article
Chemistry, Multidisciplinary
Yuna Moon, Madhumita Patel, Soyoun Um, Hyun Jung Lee, Sohee Park, Soo-Bong Park, Sun-Shin Cha, Byeongmoon Jeong
Summary: Dietary intake of folic acid promotes cartilage regeneration by enhancing the chondrogenic differentiation of tonsil-derived mesenchymal stem cells (TMSCs). The optimal concentration of folic acid treatment is 30 μM, where it significantly increases the expression of chondrogenic biomarkers without affecting osteogenic markers. However, high concentrations of folic acid may lead to a decrease in chondrogenic biomarkers, possibly due to DNA methylation.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Chemistry, Multidisciplinary
Madhumita Patel, Sujeong Ahn, Won -Gun Koh
Summary: Tissue engineering is a promising approach for reconstructing neuronal injuries, where topographical features play a crucial role in directing neuronal responses. This review discusses recent progress in the development of topographical patterns for neuronal outgrowth, guidance, and differentiation, as well as the underlying mechanisms.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
HanSol Kim, Eun Jo Jang, Narendra V. Sankpal, Madhumita Patel, Rajkumar Patel
Summary: Scientists have been studying the behavior and anatomy of various animals to gain insights into the human brain. However, due to the differences between animal and human brains, understanding how the brain works has remained challenging. Recently, brain-like organoids that mimic the characteristics of the human brain have been developed, which allows researchers to delve deeper into understanding the neurological structures and causes of brain diseases. This paper reviews the latest advancements in generating brain-like organoids using Matrigel and other alternatives, discusses gel-free methods to enhance organoid regeneration, and details the growth and development of vascularized brain organoids in both in vitro and in vivo conditions.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Chemistry, Applied
Maninder Meenu, Bandana Padhan, Madhumita Patel, Rajkumar Patel, Baojun Xu
Summary: Essential oils have gained popularity as natural food preservatives due to their potent antibacterial activity against food pathogens. This review discusses the antibacterial activity of essential oils from various plant parts and sources against important food pathogens such as Salmonella and Listeria, as well as the mechanisms behind their antibacterial activity.
Review
Materials Science, Biomaterials
Naomi Akiyama, Kapil D. Patel, Eun Jo Jang, Mark R. Shannon, Rajkumar Patel, Madhumita Patel, Adam Willis Perriman
Summary: The composition, morphology, and mechanical performance of nanomaterials are crucial for tissue engineering. Tubular nanomaterials (TNs), including carbon nanotubes, titanium oxide nanotubes, halloysite nanotubes, silica nanotubes, and hydroxyapatite nanotubes, have shown great potential in various applications due to their high surface area, versatile surface chemistry, well-defined mechanical properties, excellent biocompatibility, and monodispersity. This review focuses on the recent developments in TN-based biomaterials for structural tissue engineering, with a strong emphasis on bone tissue regeneration. It includes a detailed literature review on TN-based orthopedic coatings for metallic implants and composite scaffolds to enhance in vivo bone regeneration.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)