Journal
MATERIALS TODAY COMMUNICATIONS
Volume 26, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.mtcomm.2020.101824
Keywords
PLGA; Mesoporous silica nanoparticles; Low-temperature deposition manufacturing; Cartilage tissue engineering
Categories
Funding
- National Natural Science Foundation of China [81572198, 81772394]
- Natural Science Foundation of Guangdong Province, China [2018A030310027, 2018B0303110003, 2018A030310645]
- Shenzhen Peacock Project [KQTD20170331100838136]
- Fund for High Level Medical Discipline Construction of Shenzhen University [2016031638]
- Medical Research Foundation of Guangdong Province, China [A2016314]
- Shenzhen Science and Technology Projects [JCYJ20170306093213555, JCYJ20170 817171930009, JCYJ20170306092215436, JCYJ20170412150609690, JCYJ20170413161800287, SGLH20161209105517753, JCYJ2016030 1111338144, JCYJ20160429185235132, JCYJ20180507183036060]
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In this study, mesoporous silica nanoparticles (MSNs) were introduced into poly(lactic-co-glycolic acid) (PLGA) to prepare a novel three-dimensional porous scaffold by low-temperature deposition manufacturing (LDM). The scaffolds exhibited 3D geometry and desirable big pore structures, while maintaining the bioactivity of the biomaterials. This research demonstrated that the 3D-printed PLGA/MSNs scaffolds have promising potential in cartilage tissue engineering, with enhanced cell proliferation and mechanical properties compared to PLGA scaffolds.
In this study, we introduced mesoporous silica nanoparticles (MSNs) into poly(lactic-co-glycolic acid) (PLGA) to prepare a novel three dimensional (3D) porous scaffold by low-temperature deposition manufacturing (LDM). During the scaffolds preparing process, LDM managed to fabricate scaffolds with 3D geometry and desirable big pore structures but not damage the bioactivity of biomaterials. In the subsequent freeze-drying process, the as-fabricated scaffolds produced small pore structures due to the phase separation process. The scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectrometer, hydrophilicity, porosity and mechanical test. The mean diameters of big pores were 445 +/- 50 mu m and 431 +/- 32 mu m, and those of small pores were 11 +/- 3 mu m and 8 +/- 2 mu m for PLGA and PLGA/MSNs scaffolds, respectively. Hydrophilicity examination revealed enhanced hydrophilicity of the composite scaffolds. Besides, the incorporation of MSNs also improves the mechanical property of the composites. Human bone marrow mesenchymal stem cells (hMSCs) were seeded on scaffolds for 3, 5, and 7 days. CCK-8 assay revealed that the introduction of MSNs improved cell proliferation. The adhesion and growth of cells on PLGA/MSNs scaffolds were enhanced over time during culturing period when compared to that on PLGA scaffolds. The results suggested that the 3D-printed PLGA/MSNs scaffolds are promising in cartilage tissue engineering.
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