Article
Biochemistry & Molecular Biology
Huba L. Marton, Kathryn M. Styles, Peter Kilbride, Antonia P. Sagona, Matthew Gibson
Summary: Poly(ethylene glycol) has been identified as an effective cryoprotectant for bacteriophages, offering protection at both -20 and -80 degrees Celsius. It outperforms glycerol in many cases, with high post-thaw recoveries and confirmed lytic function against bacterial hosts. This low-cost, soluble, and well-tolerated polymer may facilitate the development of new approaches for phage therapy.
Article
Microbiology
Larissa Kever, Ael Hardy, Tom Luthe, Max Huennefeld, Cornelia Gaetgens, Lars Milke, Johanna Wiechert, Johannes Wittmann, Cristina Moraru, Jan Marienhagen, Julia Frunzke
Summary: Predation by phages is a major driver of bacterial evolution. This study reveals that aminoglycosides, a type of antibiotics produced by Streptomyces, have potent antiviral properties and can block an early step of phage infection. The supernatants from natural aminoglycoside producers also show broad inhibitory effects on phage propagation. Acetylation of the aminoglycoside antibiotic apramycin abolishes its antibacterial effect but retains its antiviral properties. These findings expand our understanding of aminoglycoside functions and suggest that they may provide protection against phage predation at the community level.
Article
Infectious Diseases
Mikael Skurnik
Summary: Due to the increasing antibiotic resistance, it is necessary to address the issue promptly. Phage therapy, as a precision therapy for bacterial infections, has gained attention, but there are still unresolved issues such as clear instructions, practical experience, and seamless coordination.
Review
Biochemistry & Molecular Biology
Jose Luis Villalpando-Aguilar, Gilberto Matos-Pech, Itzel Lopez-Rosas, Hugo Gildardo Castelan-Sanchez, Fulgencio Alatorre-Cobos
Summary: Phage therapy is an alternative treatment that uses bacteriophages to kill specific bacteria. It has shown positive results in improving food production in agriculture. However, finding effective bacteriophages is crucial for the success of this therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Aditi Singh, Sudhakar Padmesh, Manish Dwivedi, Irena Kostova
Summary: Bacteria survive and become drug-resistant by forming biofilms on various surfaces. Bacteriophages offer a promising approach to invade and destroy these biofilms. Research on phage therapy provides insights into the interaction between bacteriophages and bacterial biofilms.
INFECTION AND DRUG RESISTANCE
(2022)
Review
Medicine, Research & Experimental
Carola Venturini, Aleksandra Petrovic Fabijan, Alicia Fajardo Lubian, Stefanie Barbirz, Jonathan Iredell
Summary: Bacteriophages are selective viral predators of bacteria and can be used to treat bacterial infections. However, there are challenges to implementing phage therapy into medical practice, including a lack of clinical trial data and a need for a better understanding of bacterium-phage interactions.
EMBO MOLECULAR MEDICINE
(2022)
Article
Public, Environmental & Occupational Health
Ahlam Alsaadi, Mohammed Imam, Abdulrahman A. Alghamdi, Majed F. Alghoribi
Summary: The escalating threat of antibiotic-resistant pathogens worldwide necessitates international action and multisectoral cooperation. Bacteriophages represent a promising tool for combatting this global threat. While extensive research has been conducted on phages and their applications in Europe and the United States, phage research in the Middle East is relatively limited.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2022)
Article
Microbiology
Andre Mu, Daniel McDonald, Alan K. Jarmusch, Cameron Martino, Caitriona Brennan, Mackenzie Bryant, Gregory C. Humphrey, Julia Toronczak, Tara Schwartz, Dominic Nguyen, Gail Ackermann, Anthony D'Onofrio, Steffanie A. Strathdee, Robert T. Schooley, Pieter C. Dorrestein, Rob Knight, Saima Aslam
Summary: Our study found that adding phage therapy to systemic antibiotics for 4 weeks in a patient with Staphylococcus aureus device infection resulted in largely unchanged microbiota profile. Metabolomic analysis suggested potential indirect ecological impacts to the host microbiome. Phages used to treat the patient were not detected in metagenomic samples from saliva, stool, and skin, but were detected in patient serum using endpoint-PCR.
Article
Infectious Diseases
Aryan Rahimi-Midani, Seon-Woo Lee, Tae-Jin Choi
Summary: Bacteriophages are viruses that infect bacterial hosts, playing a crucial role in modern biotechnology and combating antibiotic-resistant microbes. With the advancement of sequencing technologies, their application is being reconsidered, although new challenges need to be addressed. More studies are required to find low-risk control methods against antimicrobial-resistant bacteria, as phage therapy is not expected to be the sole strategy.
Review
Infectious Diseases
Celia Ferriol-Gonzalez, Pilar Domingo-Calap
Summary: The irrational use of antibiotics has led to a high emergence of multi-drug resistant bacteria, particularly in the animal feed industry and companion animal infections. Phages have been proposed as an alternative for antibiotics, showing potential applications in treating infections in livestock and companion animals.
Review
Infectious Diseases
Andrew Vaitekenas, Anna S. Tai, Joshua P. Ramsay, Stephen M. Stick, Anthony Kicic
Summary: Antimicrobial resistance is a significant threat in modern healthcare, particularly for bacterial infections in individuals with chronic conditions like cystic fibrosis. Pseudomonas aeruginosa infections are a major factor in CF lung disease, with high resistance to multiple antimicrobials. Phages show promise as an alternative therapy, but their narrow host range and phage resistance evolution may limit their efficacy. Developing phage mixtures with broad host ranges to prevent resistance evolution is a potential strategy, but further research is needed on CF-derived P. aeruginosa phage resistance mechanisms for effective phage therapy formulations.
Review
Biochemistry & Molecular Biology
Abdallah Abdelsattar, Alyaa Dawoud, Salsabil Makky, Rana Nofal, Ramy Aziz, Ayman El-Shibiny
Summary: This article summarizes the applications of bacteriophages in various fields and discusses the limitations and knowledge gaps in the field. Additionally, the computational methods used in phage genome analysis are critically assessed, providing useful insights for researchers in selecting suitable approaches for phage-related research aims and applications.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alexandr D. Efimov, Alla K. Golomidova, Eugene E. Kulikov, Ilya S. Belalov, Pavel A. Ivanov, Andrey Letarov
Summary: Most enterobacterial O antigen types provide strong protection against direct recognition by bacteriophage receptor-recognition proteins. Certain bacteriophages use specific O antigens as their primary receptors and can also bind to additional receptors to infect different strains of E. coli. The mechanical force of the deployment of short tail fibers allows these phages to penetrate the O polysaccharide layer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Cheng Chang, Wennan Guo, Xinbo Yu, Chaoyi Guo, Nan Zhou, Xiaokui Guo, Ru-Lin Huang, Qingtian Li, Yongzhang Zhu
Summary: Bacteriophages, particularly M13 phages, have been extensively studied in the field of nanomedicine due to their unique biological advantages and engineering capabilities. The nanofiber-like morphology of M13 phages allows them to reach various target sites and self-assemble into multidimensional scaffolds. Genetic modifications enable the display of peptides and antibodies on the phages, making them valuable for precise and individualized medicine. Researchers have also used novel engineering approaches to enhance the bionanomaterial properties of M13 phages, leading to applications in drug delivery, biodetection, tissue regeneration, and targeted cancer therapy.
MATERIALS TODAY BIO
(2023)
Review
Infectious Diseases
Salman Sahab Atshan, Rukman Awang Hamat, Musheer A. Aljaberi, Jung-Sheng Chen, Shih-Wei Huang, Chung-Ying Lin, Benjamin J. Mullins, Anthony Kicic
Summary: The production and use of antibiotics increased significantly after the Second World War, but bacterial resistance has now become a global issue. High-risk individuals, such as burn and wound patients, as well as those with pulmonary infections caused by antibiotic-resistant bacteria, are in urgent need of antibiotics. However, with the decline in investment for new antibiotics, the search for alternative therapeutic strategies, such as bacteriophage therapy, has become crucial.
Article
Virology
Giuseppe Pisanelli, Maudry Laurent-Rolle, Balaji Manicassamy, Alan Belicha-Villanueva, Juliet Morrison, Bernardo Lozano-Dubernard, Felipa Castro-Peralta, Giuseppe Iovane, Adolfo Garcia-Sastre
Article
Microbiology
Maudry Laurent-Rolle, Juliet Morrison, Ricardo Rajsbaum, Jesica M. Levingston Macleod, Giuseppe Pisanelli, Alissa Pham, Juan Ayllon, Lisa Miorin, Carles Martinez-Romero, Benjamin R. tenOever, Adolfo Garcia-Sastre
CELL HOST & MICROBE
(2014)
Article
Immunology
T. Zottola, S. Montagnaro, C. Magnapera, S. Sasso, L. De Martino, A. Bragagnolo, L. D'Arnici, R. Condoleo, G. Pisanelli, G. Iovane, U. Pagnini
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2013)
Article
Immunology
Ricardo Rajsbaum, Gijs A. Versteeg, Sonja Schmid, Ana M. Maestre, Alan Belicha-Villanueva, Carles Martinez-Romero, Jenish R. Patel, Juliet Morrison, Giuseppe Pisanelli, Lisa Miorin, Maudry Laurent-Rolle, Hong M. Moulton, David A. Stein, Ana Fernandez-Sesma, Benjamin R. tenOever, Adolfo Garcia-Sastre
Article
Veterinary Sciences
Serena Montagnaro, Simona Sasso, Luisa De Martino, Mariangela Longo, Valentina Iovane, Glanbenedetto Ghiurmino, Giuseppe Pisanelli, Donatella Nava, Loredana Baldi, Ugo Pagnini
JOURNAL OF WILDLIFE DISEASES
(2010)
Article
Multidisciplinary Sciences
Balaji Manicassamy, Santhakumar Manicassamy, Alan Belicha-Villanueva, Giuseppe Pisanelli, Bali Pulendran, Adolfo Garcia-Sastre
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Article
Immunology
S. Montagnaro, M. Longo, K. Mallardo, G. Pisanelli, L. De Martino, G. Fusco, L. Baldi, U. Pagnini, G. Iovane
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2008)
Article
Virology
M. Longo, F. Fiorito, G. Marfe, S. Montagnaro, G. Pisanelli, L. De Martino, G. Iovane, U. Pagnini
Article
Microbiology
Juliet Morrison, Maudry Laurent-Rolle, Ana M. Maestre, Ricardo Rajsbaum, Giuseppe Pisanelli, Viviana Simon, Lubbertus C. F. Mulder, Ana Fernandez-Sesma, Adolfo Garcia-Sastre
Article
Virology
Lisa Miorin, Maudry Laurent-Rolle, Giuseppe Pisanelli, Pierre Hendrick Co, Randy A. Albrecht, Adolfo Garcia-Sastre, Juliet Morrison
JOURNAL OF VIROLOGY
(2019)
Article
Virology
Luiz Gustavo dos Anjos Borges, Giuseppe Pisanelli, Oyahida Khatun, Adolfo Garcia-Sastre, Shashank Tripathi
Review
Microbiology
Sonia Laneri, Mariarita Brancaccio, Cristina Mennitti, Margherita G. De Biasi, Maria Elena Pero, Giuseppe Pisanelli, Olga Scudiero, Raffaela Pero
Summary: Antimicrobial peptides (AMPs), such as alpha- and beta-defensins, have antiviral properties and inhibit viruses through various mechanisms. Researchers are exploring the potential of AMPs and their derivatives as therapies against COVID-19, considering their interactions with toll-like receptors (TLRs) and chemokine receptors in the context of the disease's pathogenesis. Additionally, controlled physical activity, along with a healthy lifestyle, may serve as natural defense against COVID-19 and support therapies, as seen in the increase of serum defensins in competitive athletes.
Review
Virology
Giuseppe Pisanelli, Ugo Pagnini, Giuseppe Iovane, Adolfo Garcia-Sastre
Summary: Paramyxoviruses are negative single-strand RNA viruses that can cause significant diseases in humans and animals. These viruses have evolved different strategies to evade the host's interferon defense mechanism, which is activated upon viral recognition and leads to the production of interferons that reduce viral replication. This article analyzes the current status of the various strategies used by paramyxoviruses to bypass interferon responses.
Article
Agriculture, Dairy & Animal Science
M. Longo, S. Montagnaro, L. De Martino, G. Pisanelli, R. Frontoso, S. Roperto, U. Pagnini, G. Iovane
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2007)