Journal
PATHOGENS
Volume 10, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/pathogens10040493
Keywords
biofilm formation; adhesion; GPI; cell wall proteins; Als adhesins; Candida parapsilosis; candidiasis
Categories
Funding
- Spanish Ministry of Economy and Competitiveness (MINECO) - EU (FEDER) [SAF2013-47570-P, SAF2017-86188-P]
- regional government of Castilla-La Mancha (JCCM) - EU (FEDER) [SBPLY/19/180501/000114]
- Consejeria de Educacion, Universidades e Investigacion of Gobierno Vasco-Eusko Jaurlaritza [GIC15/78 IT-990-16]
- FP7-PEOPLE-2013-ITN-Marie-Curie Action: Initial Training Networks: Molecular Mechanisms of Human Fungal Pathogen Host Interaction, ImResFun [MC-ITN-606786]
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A comprehensive study of the cell wall proteome of Candida parapsilosis revealed an increase in abundance and diversity of putative wall adhesins in isolates with high biofilm formation capacity. This increase was observed in non-smooth isolates with elongated cellular phenotypes, suggesting a correlation between high biofilm formation capacity and increased expression of wall adhesins.
Candida parapsilosis is among the most frequent causes of candidiasis. Clinical isolates of this species show large variations in colony morphotype, ranging from round and smooth to a variety of non-smooth irregular colony shapes. A non-smooth appearance is related to increased formation of pseudohyphae, higher capacity to form biofilms on abiotic surfaces, and invading agar. Here, we present a comprehensive study of the cell wall proteome of C. parapsilosis reference strain CDC317 and seven clinical isolates under planktonic and sessile conditions. This analysis resulted in the identification of 40 wall proteins, most of them homologs of known Candida albicans cell wall proteins, such as Gas, Crh, Bgl2, Cht2, Ecm33, Sap, Sod, Plb, Pir, Pga30, Pga59, and adhesin family members. Comparative analysis of exponentially growing and stationary phase planktonic cultures of CDC317 at 30 degrees C and 37 degrees C revealed only minor variations. However, comparison of smooth isolates to non-smooth isolates with high biofilm formation capacity showed an increase in abundance and diversity of putative wall adhesins from Als, Iff/Hyr, and Hwp families in the latter. This difference depended more strongly on strain phenotype than on the growth conditions, as it was observed in planktonic as well as biofilm cells. Thus, in the set of isolates analyzed, the high biofilm formation capacity of non-smooth C. parapsilosis isolates with elongated cellular phenotypes correlates with the increased surface expression of putative wall adhesins in accordance with their proposed cellular function.
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