Article
Oncology
Anna Ricarda Gruending, Marc A. Schneider, Sarah Richtmann, Mark Kriegsmann, Hauke Winter, Beatriz Martinez-Delgado, Sarai Varona, Bin Liu, David S. DeLuca, Julia Held, Sabine Wrenger, Thomas Muley, Michael Meister, Tobias Welte, Sabina Janciauskiene
Summary: The accumulation of lipid droplets (LDs) and high expression of SREBF1 are associated with drug resistance and poor prognosis in cancer. The sensitivity of lung cancer cells to chemotherapeutics cisplatin and etoposide was assessed in this study. While both drugs reduced SREBF1 expression, it did not parallel with LD formation and cell sensitivity to chemotherapeutics. However, lower SREBF1 expression in tumors correlated with better overall survival in non-small-cell lung cancer patients.
Article
Chemistry, Analytical
Francesca Pagliari, Elisa Sogne, Davide Panella, Gerardo Perozziello, Carlo Liberale, Gobind Das, Alice Turdo, Simone Di Franco, Joao Seco, Andrea Falqui, Santo Gratteri, Arturo Pujia, Enzo Di Fabrizio, Patrizio Candeloro, Luca Tirinato
Summary: Melanoma is a type of tumor with a high percentage of cancer stem cells. Researchers have identified lipid droplets as one of the markers for these cells. They have used various techniques, such as mass spectrometry and imaging techniques, to characterize the droplets and developed a new experimental approach to study them.
Article
Cell Biology
Krzysztof Czamara, Marta Stojak, Marta Z. Pacia, Alicja Zieba, Malgorzata Baranska, Stefan Chlopicki, Agnieszka Kaczor
Summary: Endothelial inflammation induced by LPS is closely related to the formation of LDs, with LD composition depending on LPS incubation time and the presence of serum. This study highlights the spatial association of LPS-induced LDs with nanostructural changes in cell membrane architecture.
Article
Biochemistry & Molecular Biology
Pradjna N. Paramitha, Riki Zakaria, Anisa Maryani, Yukako Kusaka, Bibin B. Andriana, Kosuke Hashimoto, Hiromitsu Nakazawa, Satoru Kato, Hidetoshi Sato
Summary: The study revealed that oleic and linoleic acids promote higher rates of fat accumulation in hepatic cells compared to palmitic and stearic acids, with linoleic acid causing cell death while oleic acid does not. Additionally, linoleic fatty chains taken into the cells are modified into oleic fatty chains, suggesting a potential role in inflammation in nonalcoholic fatty liver disease (NAFLD).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Boram Choi, Yoojin Na, Min Yeop Whang, Jung Yoon Ho, Mi-Ryung Han, Seong-Woo Park, Heekyoung Song, Soo Young Hur, Youn Jin Choi
Summary: In this study, the association between MGMT methylation and persistent HPV infection was examined using cervical cytology samples and a public database. The results showed that MGMT methylation was associated with HPV infection and downregulated MGMT mRNA expression correlated with poor overall survival. MGMT methylation level could potentially serve as a prognostic indicator for the transition from atypical squamous cells/low-grade squamous intraepithelial lesion to cervical cancer.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Spectroscopy
Chi Zhang
Summary: Lipid droplets (LDs) are crucial for maintaining cellular metabolic homeostasis, and studying their functions and changes in various pathological conditions is important for diagnosis and treatment. Coherent Raman scattering (CRS) microscopy has been used to image and quantify LDs in live cells, offering faster imaging speed and similar chemical information compared to spontaneous Raman scattering microscopy. LDs, with their high lipid density, have strong CRS signals, making them the most widely studied organelle in the CRS field. This review discusses recent achievements in using CRS to study the quantity, distribution, composition, and dynamics of LDs in different systems.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Review
Cell Biology
Monica A. Farias, Benjamin Diethelm-Varela, Areli J. Navarro, Alexis M. Kalergis, Pablo A. Gonzalez
Summary: This article reviews and discusses the interplay between neutral lipid metabolism and lipid droplets (LDs) in the replication cycle of different DNA viruses. It highlights the role of LDs in modulating viral infection and identifies potentially new molecular targets for the treatment of viral infections.
Article
Chemistry, Multidisciplinary
Han Zhao, Donghan Ma, Junkai Xie, Oscar Sanchez, Fang Huang, Chongli Yuan
Summary: This study developed a novel probe targeting methylated mtDNA, demonstrating its capability to reveal the spatial distribution and changes of mtDNA methylation at a single-cell level. The study also tracked single-cell mtDNA and nDNA methylation simultaneously and revealed unsynchronized dynamics between the nucleus and mitochondria. This tool provides a unique opportunity to understand the epigenetic regulation of mtDNA and its dynamic response to the microenvironment and cellular changes.
Article
Biochemistry & Molecular Biology
Marta Z. Pacia, Natalia Chorazy, Magdalena Sternak, Benedikt Fels, Michal Pacia, Mariusz Kepczynski, Kristina Kusche-Vihrog, Stefan Chlopicki
Summary: In this study, the role of Rac1 in TNF-induced vascular inflammation was investigated. It was found that Rac1 mediated the formation of lipid droplets and nanostructural alterations associated with vascular inflammation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Borek-Dorosz, Anna Pieczara, Krzysztof Czamara, Marta Stojak, Ewelina Matuszyk, Katarzyna Majzner, Krzysztof Brzozowski, Arianna Bresci, Dario Polli, Malgorzata Baranska
Summary: In this study, the functionality of inflamed cells was verified by analyzing their ability to uptake and accumulate exogenous saturated fatty acids. The results showed that the uptake of fatty acids in inflamed cells is dependent on their concentration and type, providing a better understanding of endothelial cell inflammation and its impact on their fat buffering capacity. The microscopic methods used in the analysis showed complementarity in studying fatty acid uptake by endothelial cells from different perspectives.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Food Science & Technology
Laura Bordoni, Anna M. Malinowska, Irene Petracci, Artur Szwengiel, Rosita Gabbianelli, Agata Chmurzynska
Summary: This study explores the potential association between TMA metabolism and mitochondrial dynamics, providing a new avenue for further research.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Bradley A. Ruple, Joshua S. Godwin, Paulo H. C. Mesquita, Shelby C. Osburn, Christopher G. Vann, Donald A. Lamb, Casey L. Sexton, Darren G. Candow, Scott C. Forbes, Andrew D. Fruge, Andreas N. Kavazis, Kaelin C. Young, Robert A. Seaborne, Adam P. Sharples, Michael D. Roberts
Summary: Resistance training (RT) has been shown to impact the mitochondrial DNA (mtDNA) methylome in older males, leading to increased lean tissue mass, muscle thickness, and strength. The study observed reduced methylation and more youthful signatures in mtDNA sites of older males post-RT compared to younger males. These findings suggest that RT may enhance mitochondrial transcription levels and induce mitochondrial methylome profiles resembling those of younger men.
Article
Oncology
Chiara Herzog, Karin Sundstrom, Allison Jones, Iona Evans, James E. Barrett, Jiangrong Wang, Elisa Redl, Lena Schreiberhuber, Laura Costas, Sonia Paytubi, Lukas Dostalek, Michal Zikan, David Cibula, Gaby Sroczynski, Uwe Siebert, Joakim Dillner, Martin Widschwendter
Summary: This study developed an automatable triage test for cervical screening that is highly sensitive and specific in predicting the risk of progression to CIN3+ in HPV+ patients. The test demonstrated a sensitivity of 100% for detecting invasive cancer and 78% for detecting CIN3, with a specificity of 90%. It also identified 52% of women aged 30 and above with a negative cytology index sample who were later diagnosed with CIN3 within 1-4 years.
CLINICAL EPIGENETICS
(2022)
Article
Spectroscopy
Anna Pieczara, Ewelina Matuszyk, Piotr Szczesniak, Jacek Mlynarski, Malgorzata Baranska
Summary: The role of mitochondria in cellular function, including the production of reactive oxygen species and the regulation of cell death, is significant. This study utilizes Raman microscopy to locate and track functional changes in mitochondria, and explores the potential of using Raman probes for cell labeling to improve specificity and sensitivity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Endocrinology & Metabolism
Amanda L. Morin, Phyo W. Win, Angela Z. Lin, Christina A. Castellani
Summary: Bidirectional crosstalk between the nuclear and mitochondrial genomes is crucial for cell functioning. Mitochondrial DNA copy number and heteroplasmy can influence mitochondrial function, nuclear genome, and contribute to overall health and disease. Mitochondria act as environmental biosensors, conveying essential information about cell state to the nuclear genome. Cellular communication between mitochondrial variation and nuclear epigenome can occur through modifications of metabolites and intermediates, affecting epigenetic modifications.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zuzanna Majka, Krzysztof Czamara, Joanna Janus, Mariusz Kepczynski, Agnieszka Kaczor
Summary: This study used Raman techniques and fluorescence imaging to investigate the effects of high-fat diet on different adipose tissue depots in young and mature mice. The results showed a synergistic effect of age and high-fat diet-induced obesity on adipocyte morphology and lipid composition. Short-term high-fat diet mainly caused hypertrophy of periaortic adipose tissue, while prolonged exposure to harmful factors led to chemical changes in this tissue. Brown adipose tissue remained resistant to high-fat diet, highlighting its potential as a target for obesity therapies.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Katarzyna Sitarz, Krzysztof Czamara, Slawa Szostek, Agnieszka Kaczor
Summary: Reinterpretation of the Warburg effect has led to a better understanding of aerobic glycolysis providing molecular precursors necessary for the continuous growth and rapid proliferation of cancer cells. The close link between HPV and cancer suggests that HPV impacts cellular metabolism through activation of metabolic pathways and direct influence on enzymes, promoting the Warburg effect.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Biochemistry & Molecular Biology
Ewa Stanek, Marta Z. Pacia, Agnieszka Kaczor, Krzysztof Czamara
Summary: Spectroscopy-based analysis of cells' chemical composition provides unique information about cell identity. Through monitoring stem cell differentiation, researchers have found significant chemical and functional changes. However, stem cell-derived adipocytes have a different chemical composition compared to mature primary adipocytes.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Krzysztof Czamara, Zuzanna Majka, Ewa Stanek, Natalia Hachlica, Agnieszka Kaczor
Summary: This review discusses the recent advancements in using Raman-based techniques for analyzing adipose tissue, with a specific focus on perivascular adipose tissue (PVAT) and its pathological changes. The review critically evaluates the potential and limitations of Raman techniques in diagnosing targeted adipose tissues and developing anti-obesity therapies.
PROGRESS IN LIPID RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Natalia Hachlica, Michal Rawski, Marcin Gorecki, Aleksandra Wajda, Agnieszka Kaczor
Summary: In this study, we characterized the organization levels of amyloid fibrils from proteins with similar structures using various methods including VCD, ECD, cryo-EM, and TEM. Our results showed that small changes in protein structure or preparation conditions led to significant differences in the structure and architecture of the fibrils. Although the secondary structure and protofilament twist varied, the mesoscopic structure of the fibrils remained relatively similar. These findings contribute to understanding the indeterministic nature of fibril formation.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Aleksandra Kolodziejczyk, Laurence A. Nafie, Aleksandra Wajda, Agnieszka Kaczor
Summary: Amyloid fibrils exhibit multi-layered chiral supramolecular structures and are highly sensitive to vibrational circular dichroism (VCD) for fibril formation. In this study, we demonstrate an exceptionally large enhancement of Raman optical activity (ROA) signal in mature amyloid fibrils and their prefibrillar states. This enhanced ROA signal is observed in fibrils of different proteins, indicating a possible general phenomenon. The ROA signal appears earlier than VCD and provides access to a broader range of vibrations, suggesting its potential utility for analyzing amyloid fibrils.
CHEMICAL COMMUNICATIONS
(2023)
Article
Spectroscopy
Natalia Hachlica, Aleksandra Kolodziejczyk, Michal Rawski, Marcin Gorecki, Aleksandra Wajda, Agnieszka Kaczor
Summary: Environmental factors can compete against the templated growth of amyloid fibrils, and the structural indeterminism originates from deeper levels of the fibril structure.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Chemistry, Multidisciplinary
Aleksandra Orlef, Ewa Stanek, Krzysztof Czamara, Aleksandra Wajda, Agnieszka Kaczor
Summary: The aggregation-sensitive chiroptical output, provided by enantiopure (3S,3 ' S)-astaxanthin, is utilized to investigate and control carotenoid assembling processes in Pluronic F-127 nanoparticles. The formation of kinetically stabilized H1 self-assemblies outside the micelles interferes with the process of carotenoid J-aggregation inside nanocarriers. Nanocarriers with encapsulated stable J-aggregates offer controlled release of carotenoid molecules to primary murine adipocytes.
CHEMICAL COMMUNICATIONS
(2022)