Journal
FRONTIERS IN IMMUNOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.638907
Keywords
Lentivirus; Infection temperature; chimeric antigen receptor (CAR); naive T cells; immune checkpoints
Categories
Funding
- General Project of National Natural Science Foundation of China [81970180]
- Key Science and Technology Support Project of Tianjin Science and Technology Bureau [20YFZCSY00800]
- Tianjin First Central Hospital
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This study found that infection temperature affects the phenotype and function of CAR-T cells, recommending infection at 32 degrees for preparing CAR-T cells with a balanced function and phenotype, which have enhanced oncolytic ability.
Chimeric antigen receptor (CAR)-T cell therapy has become an important method for the treatment of hematological tumors. Lentiviruses are commonly used gene transfer vectors for preparing CAR-T cells, and the conditions for preparing CAR-T cells vary greatly. This study reported for the first time the influence of differences in infection temperature on the phenotype and function of produced CAR-T cells. Our results show that infection at 4 degrees produces the highest CAR-positive rate of T cells, infection at 37 degrees produces the fastest proliferation in CAR-T cells, and infection at 32 degrees produces CAR-T cells with the greatest proportion of naive cells and the lowest expression of immune checkpoints. Therefore, infection at 32 degrees is recommended to prepare CAR-T cells. CAR-T cells derived from infection at 32 degrees seem to have a balance between function and phenotype. Importantly, they have increased oncolytic ability. This research will help optimize the generation of CAR-T cells and improve the quality of CAR-T cell products.
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