Journal
ADVANCED ELECTRONIC MATERIALS
Volume 7, Issue 5, Pages -Publisher
WILEY
DOI: 10.1002/aelm.202001114
Keywords
biosensors; carbon nanotubes; electrolyte‐ gated transistors; green chemistry; lysozymes
Funding
- Horizon 2020 Framework Programme [FETOPEN-801367 evFOUNDRY]
- FIRC-AIRC fellowship for Italy [22318]
- AIRC under MFAG 2019 [22894]
- PRIN2017-NiFTy [2017MYBTXC]
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A general single-step approach has been introduced for the green fabrication of hybrid biosensors from water dispersion, successfully integrating the semiconducting properties of a carbon nanotube (CNT) and the functionality of a protein. This methodology allows for the assessment of EGT response to biorecognition and extraction of protein-substrate binding constant, showing promise for a new class of CNT-protein biosensors.
A general single-step approach is introduced for the green fabrication of hybrid biosensors from water dispersion. The resulting device integrates the semiconducting properties of a carbon nanotube (CNT) and the functionality of a protein. In the initial aqueous phase, the protein (viz., lysozyme [LZ]) disperses the (6,5)CNT. Drop-casting of the dispersion on a test pattern (a silicon wafer with interdigitated Au source and drain electrodes) yields a fully operating, robust, electrolyte-gated transistor (EGT) in one step. The EGT response to biorecognition is then assessed using the LZ inhibitor N-acetyl glucosamine trisaccharide. Analysis of the output signal allows one to extract a protein-substrate binding constant in line with values reported for the free (without CNT) system. The methodology is robust, easy to optimize, redirectable toward different targets and sets the grounds for a new class of CNT-protein biosensors that overcome many limitations of the technology of fabrication of CNT biosensors.
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