Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 171, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/61453
Keywords
-
Categories
Funding
- NIH Office of Research Infrastructure Programs [P40 OD010440]
- NSF Living Collections CSBR [1930382]
- NIH [U2CES030167]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1930382] Funding Source: National Science Foundation
Ask authors/readers for more resources
This study presents a method for culturing and collecting a large population of mixed-stage C. elegans on a large-scale culture plate, suitable for integration into large-scale research on model organisms. The method provides sufficient animals for research and enables comparative analyses across multiple platforms, ensuring overall reproducibility of the study.
Caenorhabditis elegans (C. elegans) has been and remains a valuable model organism to study developmental biology, aging, neurobiology, and genetics. The large body of work on C. elegans makes it an ideal candidate to integrate into large-population, whole-animal studies to dissect the complex biological components and their relationships with another organism. In order to use C. elegans in collaborative - omics research, a method is needed to generate large populations of animals where a single sample can be split and assayed across diverse platforms for comparative analyses. Here, a method to culture and collect an abundant mixed-stage C. elegans population on a large-scale culture plate (LSCP) and subsequent phenotypic data is presented. This pipeline yields sufficient numbers of animals to collect phenotypic and population data, along with any data needed for -omics experiments (i.e., genomics, transcriptomics, proteomics, and metabolomics). In addition, the LSCP method requires minimal manipulation to the animals themselves, less user preparation time, provides tight environmental control, and ensures that handling of each sample is consistent throughout the study for overall reproducibility. Lastly, methods to document population size and population distribution of C. elegans life stages in a given LSCP are presented.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available