Article
Medicine, General & Internal
Julie Vanderelst, Alexandre Rousseau, Nicolas Selvais, Patrick Biston, Karim Zouaoui Boudjeltia, Michael Piagnerelli
Summary: The study found that during sepsis, there are changes in the shape and deformability of red blood cells, as well as a decrease in the expression of the CD35 membrane protein. These changes may contribute to the phagocytosis of red blood cells and the development of anemia in sepsis.
FRONTIERS IN MEDICINE
(2022)
Article
Multidisciplinary Sciences
Julian Eder, Leonie Schumm, Jakob P. P. Armann, Milo A. A. Puhan, Felix Beuschlein, Clemens Kirschbaum, Reinhard Berner, Nicole Toepfner
Summary: This study investigated the morphological and mechanical characteristics of red blood cells (RBCs) after SARS-CoV-2 infection in children and adolescents using real-time deformability-cytometry (RT-DC), and explored the relationship between RBC alterations and the clinical course of COVID-19. The results showed that the median RBC deformation was significantly increased in SARS-CoV-2-seropositive children and adolescents until 6 months post-COVID-19, indicating a mild course of the disease.
SCIENTIFIC REPORTS
(2023)
Review
Hematology
J. L. Vives Corrons, L. Berga Casafont, E. Feliu Frasnedo
Summary: The average lifespan of a human red blood cell is around 120 days. At this point, the cell is usually worn out and undergoes phagocytosis by macrophages in the spleen or liver, where iron is recycled and hem molecule is eliminated as bilirubin.
ANNALS OF HEMATOLOGY
(2021)
Article
Biochemical Research Methods
Qiaodong Wei, Ying Xiong, Yuhang Ma, Deyun Liu, Yunshu Lu, Shenghong Zhang, Xiaolong Wang, Huaxiong Huang, Yingbin Liu, Ming Dao, Xiaobo Gong
Summary: The physical and mechanical properties of red blood cells (RBCs) play an important role in disease diagnosis, but it is challenging to measure these properties accurately due to the unique shape and high heterogeneity of RBCs. In this study, a microfluidic technology combined with machine learning is developed to measure the mechanical properties of individual RBCs. The method can detect subtle changes in RBC components in response to different doses of drugs and explore the correlation between glycosylated hemoglobin and RBC mechanical properties. This approach has the potential to offer novel label-free single-cell-assay-based biophysical markers for RBCs, enhancing the potential for more robust disease diagnosis.
Article
Pharmacology & Pharmacy
Allan Radaic, Nam E. Joo, Soo-Hwan Jeong, Seong- Yoo, Nicholas Kotov, Yvonne L. Kapila
Summary: The study demonstrated the potential anticancer effects of phosphatidylserine-caped gold nanoparticles on breast and prostate cancer cells by inducing apoptosis, suggesting a novel therapeutic approach for cancer treatment.
Article
Cell Biology
Mei-Shin Kuo, Cheng-Hsi Chuang, Han-Chih Cheng, Hui-Ru Lin, Jong-Shyan Wang, Kate Hsu
Summary: GP.Mur is a clinically important red blood cell phenotype in Southeast Asia, influencing the structure and function of red blood cells by promoting band 3 expression and altering the organization of band 3 complexes. GP.Mur+ RBCs are less deformable, while microcytosis and membrane cholesterol reduce red blood cell deformability.
Article
Multidisciplinary Sciences
Mario Gutierrez, Mark Shamoun, Katie Giger Seu, Tyler Tanski, Theodosia A. Kalfa, Omolola Eniola-Adefeso
Summary: In this study, a parameterization model of ektacytometry was used to quantify the bulk rigidity of rigid red blood cell populations in sickle cell disease patients. By coupling this model with an artificially rigid RBC model, an innovative system was formulated to estimate the average rigidity of rigid RBC populations in SCD blood, potentially offering valuable insights into disease severity assessment and treatment.
SCIENTIFIC REPORTS
(2021)
Article
Biophysics
Zixiang Leonardo Liu, He Li, Yuhao Qiang, Pierre Buffet, Ming Dao, George Em Karniadakis
Summary: Heated erythrocytes have been used as probes to study spleen tissue and the retention ability of the spleen for over three decades. Through computational studies, it has been shown that heated RBCs exhibit deformability deterioration similar to certain diseased RBCs, providing a potential model for studying abnormal traversal dynamics and hemorheology. The rheology test expands existing data and highlights the differences between heated RBC suspensions and certain diseased RBC suspensions.
BIOPHYSICAL JOURNAL
(2021)
Article
Hematology
Steven Kho, Nurjati C. Siregar, Labibah Qotrunnada, Aurelie Fricot, Abdoulaye Sissoko, Putu A. I. Shanti, Freis Candrawati, Noy N. Kambuaya, Hasrini Rini, Benediktus Andries, David Hardy, Nur I. Margyaningsih, Fauziyah Fadllan, Desandra A. Rahmayenti, Agatha M. Puspitasari, Leo Leonardo, Bagus T. G. Yayang, Dewi S. Margayani, Pak Prayoga, Leily Trianty, Enny Kenangalem, Ric N. Price, Tsin W. Yeo, Gabriela Minigo, Rintis Noviyanti, Jeanne R. Poespoprodjo, Nicholas M. Anstey, Pierre A. Buffet
Summary: Splenomegaly in malaria patients is associated with retention of uninfected red blood cells in the spleen, which is the major mechanism of anemia.
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Article
Environmental Sciences
Jing Zhan, Qian S. Liu, Yuzhu Zhang, Zhendong Sun, Qunfang Zhou, Guibin Jiang
Summary: In this study, the erythrocytotoxicity of silica nanoparticles (SiNPs) on mouse red blood cells (RBCs) was investigated. The results showed that SiNPs induced hemolysis, morphological changes, and phosphatidylserine (PS) exposure in RBCs in a size-related manner. Further investigations revealed that SiNP-60 exposure increased ROS generation, phosphorylation of p38 and ERK1/2, and triggered platelet activation dependent on PS externalization in RBCs and thrombin formation.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biology
Nadezhda A. Besedina, Elisaveta A. Skverchinskaya, Stanislav Shmakov, Alexander S. Ivanov, Igor Mindukshev, Anton S. Bukatin
Summary: Simulating red blood cell microcirculation under oxidative stress using microfluidics revealed cell heterogeneity. While most cells exhibited membrane deformation, increased stiffness, and adhesion, resulting in decreased transit velocity in microfluidic channels, there were still cells that retained their normal biophysical characteristics and ability to move in microcapillaries.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Analytical
Yue Chen, Qiong Zhan, Jian Zhang, Wei Wang, Bee Luan Khoo, Zhen Liu, Siqi Wei, Junxin Niu, Jun Xu, Chia-Chen Yu, Xiumei Hu, Yanhui Liu, Jongyoon Han, Shuwen Liu, Lihong Liu
Summary: The drug-induced diverse response among patients is a severe problem for improving hemorheological character. However, there is no validated method for personalized therapy to the best of our knowledge. Here, we apply a gravity-driven deformability cytometry platform (GD-DCP) to profile the drug response of the red cell deform -ability (RCD) at the single-cell level using pentoxifylline (PTX) as a model drug, the effect of different concen-trations of PTX (0, 2, 20, 200 mu g mL(-1), the clinical dosage of PTX is 20 mu g mL(-1)) on RCD in patients with cardiovascular disease was explored. Based on the GD-DCP, about 38 and 56% of the acute phase of acute myocardial infarction (AMI) patients in the acute phase and coronary heart disease (CHD) patients respond positively to PTX, respectively, indicating that PTX has a strong patient dependency on RCD. Moreover, RCD is observed to be significantly inversely correlated with the activation of membrane protein kinase C (PKC) as well as the concentration of Ca2+ (both P < 0.001). The results of animal experiments show that the protective effects of PTX on myocardial ischemia rats have substantial individual variation, too. It is noted that the effect of PTX is highly consistent between RCD in vitro and in vivo outcomes (blood viscosity, myocardial injury, and electro-cardiogram (ECG)) in the same rat. All these new findings suggest that the GD-DCP is a promising method that uses deformability in vitro as one of the important criteria in personalized medicine, and our study provides unique insight into the individual-dependent mechanisms of PTX for improving RCD.
ANALYTICA CHIMICA ACTA
(2022)
Article
Hematology
Yoritaka Fujii, Makoto Taniguchi, Shingo Nagaya, Yoshibumi Ueda, Chieko Hashizume, Ken Watanabe, Hiroyuki Takeya, Takeo Kosaka, Toshiro Okazaki
Summary: Deficiency of SMS1 in mice leads to depletion of sphingomyelin on platelet membrane microdomains, resulting in excessive phosphatidylserine externalization, increased platelet clearance, and ultimately causing hemostasis dysfunction.
Review
Pediatrics
Andrea H. Gillespie, Allan Doctor
Summary: Red blood cells play important roles in hemostasis through mechanisms such as influencing blood viscosity and flow dynamics, surface interactions, generation of microparticles, and participation in blood clot formation.
FRONTIERS IN PEDIATRICS
(2021)
Article
Multidisciplinary Sciences
Marijke Grau, Lennart Kuck
Summary: Antibody labeling of RBC proteins is a commonly used method to detect protein content changes and acute alterations in protein activation states. It has various applications in assessing RBC treatments, characterizing disease states, and describing cellular coherencies.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)