Journal
CATALYSTS
Volume 11, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/catal11050560
Keywords
ionic exchange; enzyme immobilization; enzyme stability; effect of immobilization medium on enzyme immobilized stability
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Funding
- Ministerio de Ciencia e Innovacion-Spanish Government [CTQ2017-86170-R]
- ERC consolidator grant [648026]
- Guangzhou Elite Project
- European Research Council (ERC) [648026] Funding Source: European Research Council (ERC)
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This study investigated the immobilization of rAaeUPO enzyme from Agrocybe aegerita. The findings showed that the immobilization pH significantly influences the stability and activity of the immobilized enzyme.
This paper outlines the immobilization of the recombinant dimeric unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). The enzyme was quite stable (remaining unaltered its activity after 35 h at 47 degrees C and pH 7.0). Phosphate destabilized the enzyme, while glycerol stabilized it. The enzyme was not immobilized on glyoxyl-agarose supports, while it was immobilized albeit in inactive form on vinyl-sulfone-activated supports. rAaeUPO immobilization on glutaraldehyde pre-activated supports gave almost quantitative immobilization yield and retained some activity, but the biocatalyst was very unstable. Its immobilization via anion exchange on PEI supports also produced good immobilization yields, but the rAaeUPO stability dropped. However, using aminated agarose, the enzyme retained stability and activity. The stability of the immobilized enzyme strongly depended on the immobilization pH, being much less stable when rAaeUPO was adsorbed at pH 9.0 than when it was immobilized at pH 7.0 or pH 5.0 (residual activity was almost 0 for the former and 80% for the other preparations), presenting stability very similar to that of the free enzyme. This is a very clear example of how the immobilization pH greatly affects the final biocatalyst performance.
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