Article
Biology
Mallory Wiggans, Shu Jun Zhu, Alyssa M. Molinaro, Bret J. Pearson
Summary: Our study provides functional insights into how the BAF complex contributes to cell fate decisions in planarian ASCs in vivo, showing that it promotes chromatin accessibility and facilitates transcription at target loci, enabling access to genes required for the generation of certain cell lineages. However, BAF complex knockdowns do not affect the formation of endodermal lineages.
Article
Biology
Christine Schleif, Rachel Gewiss, Michael Griswold
Summary: Spermatogonial differentiation is regulated by retinoic acid and involves significant morphological and transcriptional changes. This study aimed to investigate the timing and dynamics of chromatin organization during spermatogonial development and its relationship with transcriptional changes. By using ATAC-seq, the researchers found that 50% of accessible regions in undifferentiated spermatogonia were condensed within 18 hours of retinoic acid action. Surprisingly, genes relevant to spermatogonial development remained accessible at all times, indicating that chromatin state does not directly impact transcription.
Review
Developmental Biology
Natalie J. Milmoe, Abigail S. Tucker
Summary: During head development, tissues undergo cell fate transitions to form structures, where neural crest cells play a crucial role in shaping skeletal tissues. These cells exhibit flexibility in transitioning back and forth between epithelial and mesenchymal states in the eye and ear. Understanding the factors triggering these transitions and the consequences of aberrant transitions is important for studying craniofacial development.
Article
Developmental Biology
Mingjie Xu, Jie Yao, Yingchao Shi, Huijuan Yi, Wukui Zhao, Xinhua Lin, Zhongzhou Yang
Summary: The study identified a crucial role of the SRCAP chromatin remodeling complex in mitochondrial maturation and oxidative metabolism during heart development in mice. The complex functions through H2A.Z deposition to activate transcription of metabolic genes, shedding new light on the transcriptional regulation of ubiquinone biosynthesis.
Review
Cell Biology
Mir Hilal Ahmad, Balaram Ghosh, Moshahid Alam Rizvi, Mansoor Ali, Loveleena Kaur, Amal Chandra Mondal
Summary: Neuroblastoma is a common heterogeneous extracranial cancer in infancy that is associated with aberrant activation of the Wnt signaling pathway. The Wnt signaling pathway regulates the development and differentiation of neural crest cells and affects the proliferation and metastasis of neuroblastoma. Inhibiting the Wnt signaling pathway can induce apoptosis and prevent the occurrence of neuroblastoma.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Oncology
Masayuki Hagiwara, Yota Yasumizu, Nami Yamashita, Hasan Rajabi, Atsushi Fushimi, Mark D. Long, Wei Li, Atrayee Bhattacharya, Rehan Ahmad, Mototsugu Oya, Song Liu, Donald Kufe
Summary: MUC1-C associates with E2F1 and activates a novel pathway related to prostate cancer stem cell function.
Review
Cell Biology
Ying Ye, Xi Chen, Wensheng Zhang
Summary: Embryonic stem cells have a unique ability to maintain and regulate the balance between self-renewal and multi-lineage cellular differentiation, largely dependent on gene expression regulations. Chromatin remodeling complexes play a crucial role in promoting chromatin structural changes that ultimately affect ESC fate choices.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Zhaohui Wei, Qiang Hong, Zijiao Ding, Jingwen Liu
Summary: The study generated mutant lines for cxcl12a and cxcr4b using the CRISPR/Cas9 system. Results showed that Cxcl12a is crucial for pharyngeal cartilage formation by promoting the proliferation of craniofacial neural crest cells. Additionally, Cxcl12a and Cxcl12b were found to have a synergistic influence on pharyngeal arch and pouch formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Physiology
Nayoung Ha, Jian Sun, Qian Bian, Dandan Wu, Xudong Wang
Summary: This study reveals that Hdac4 plays a regulatory role in craniofacial skeletal development by positively regulating the proliferation of cranial neural crest cells (CNCC)-derived osteoblasts. Knocking out Hdac4 leads to decreased frontal bone formation and dysregulation of cell cycle-related genes.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yanli Cheng, Zhongtian Shen, Yaqi Gao, Feilong Chen, Huisha Xu, Qinling Mo, Xinlei Chu, Chang-liang Peng, Takese T. McKenzie, Bridgitte E. Palacios, Jian Hu, Hao Zhou, Jiafu Long
Summary: The study reveals that the binding of oncoprotein SS18-SSX to chromatin remodeling complex and its phase separation are crucial for the transformation activity in synovial sarcomas.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Zachary T. Olmsted, Janet L. Paluh
Summary: Researchers have successfully studied the co-development of the central and peripheral nervous systems with trunk mesendoderm in elongating multi-lineage organized (EMLO) gastruloids. They identified NCCs that differentiate to form peripheral neurons integrated with an upstream spinal cord region after day 8.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Vanessa Polanetzki, Franziska Froeb, Tina Baroti, Margit Schimmel, Ernst R. Tamm, Michael Wegner
Summary: The study suggests that Pbrm1 and the PBAF complex are dispensable for proper Schwann cell development, and previous observed defects in Schwann cells upon Brg1 deletion are mainly due to altered or absent function of the BAF complex.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Saeid Mohammad Parast, Deli Yu, Chunxu Chen, Amanda J. Dickinson, Chenbei Chang, Hengbin Wang
Summary: In this study, the researchers investigated the role of RSF1 in early embryonic development using Xenopus laevis as a model organism. They found that knockdown of RSF1 resulted in defects in gastrulation as well as neural and neural crest development. Furthermore, they demonstrated that the recognition of the H2AK119ub mark by RSF1 is important for its function during embryonic development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Genetics & Heredity
Alfredo M. Valencia, Akshay Sankar, Pleuntje J. van der Sluijs, F. Kyle Satterstrom, Jack Fu, Michael E. Talkowski, Samantha A. Schrier Vergano, Gijs W. E. Santen, Cigall Kadoch
Summary: DNA sequencing studies of neurodevelopmental disorders (NDDs) have revealed that genes encoding mammalian SWI/SNF complexes have the highest number of genetic variants. These variants disrupt specific structural regions and contribute to the severity of NDDs. This research provides valuable insights for understanding the chromatin landscape and clinical genetics of NDDs.
Article
Cell Biology
Byung Chul Jung, Dongjoo You, Ikjun Lee, Daofeng Li, Rebecca L. Schill, Katherine Ma, Anna Pi, Zehan Song, Wei-Chieh Mu, Ting Wang, Ormond A. Macdougald, Alexander S. Banks, Sona Kang
Summary: This study reveals the significant role of TET3 in the development and expansion of white adipose tissue. Depletion of Tet3 reduces adipogenesis, protects against diet-induced adipose expansion, and enhances whole-body metabolism. The study also highlights the importance of DNA demethylation in gene regulation.
Article
Neurosciences
Godwin Sokpor, Joachim Rosenbusch, Ajaya J. Kunwar, Michael Rickmann, Tran Tuoc, Silvio O. Rizzoli, Victor Tarabykin, Gabriele Fischer von Mollard, Kerstin Krieglstein, Jochen F. Staiger
Summary: Vti1a/1b play key roles in regulating neuronal growth and are essential for proper cortical development. Deletion of Vti1a/1b results in cortical dysgenesis in mice, particularly affecting the organization of cortical layer 5 and leading to neuronal apoptosis.
Article
Cardiac & Cardiovascular Systems
Masum M. Mia, Dasan Mary Cibi, Siti Aishah Binte Abdul Ghani, Anamika Singh, Nicole Tee, Viswanathan Sivakumar, Hanumakumar Bogireddi, Stuart A. Cook, Junhao Mao, Manvendra K. Singh
Summary: Yap/Taz play an important regulatory role in MI-induced cardiac fibrosis by controlling fibroblast proliferation, transdifferentiation into myofibroblasts, and fibroinflammatory program.
CARDIOVASCULAR RESEARCH
(2022)
Article
Cell Biology
Madhulika Tripathi, Brijesh Kumar Singh, Elisa A. Liehn, Sheau Yng Lim, Keziah Tikno, David Castano-Mayan, Chutima Rattanasopa, Pakhwan Nilcham, Siti Aishah Binte Abdul Ghani, Zihao Wu, Syaza Hazwany Azhar, Jin Zhou, Sauri Hernandez-Resendiz, Gustavo E. Crespo-Avilan, Rohit Anthony Sinha, Benjamin Livingston Farah, Kyaw Thu Moe, Deidre Anne De Silva, Veronique Angeli, Manvendra K. Singh, Roshni R. Singaraja, Derek J. Hausenloy, Paul Michael Yen
Summary: Caffeine reduces vascular smooth muscle cell proliferation and restenosis by stimulating autophagy and inhibiting WNT signaling.
Review
Neurosciences
Godwin Sokpor, Beate Brand-Saberi, Huu Phuc Nguyen, Tran Tuoc
Summary: Cell delamination plays a crucial role in cortical development, and improper delamination can result in progenitor cell fate commitment and neuronal migration abnormalities. Many factors regulate cell attachment and delamination processes, and they are associated with neurodevelopmental disorders.
FRONTIERS IN NEUROSCIENCE
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Megan F. Veltri, Emily L. Durham, Kate M. Lesciotto, Abigail Coupe, Mizuho Kawasaki, Susan Motch-Perrine, Anastassia Stoykova, Tran C. Tuoc, Kazuhiko Kawasaki, Joan T. Richtsmeier
Meeting Abstract
Biochemistry & Molecular Biology
Abigail Coupe, Emily L. Durham, Kate M. Lesciotto, Megan F. Veltri, Mizuho Kawasaki, Susan Motch-Perrine, Anastassia Stoykova, Tran C. Tuoc, Kazuhiko Kawasaki, Joan T. Richtsmeier
Review
Biochemistry & Molecular Biology
Masum M. Mia, Manvendra K. Singh
Summary: This review summarizes recent findings on the involvement of the Hippo signaling pathway in modulating the immune response in different acute and chronic inflammatory diseases, and its impact on tissue repair and remodeling.
Article
Gastroenterology & Hepatology
Madhulika Tripathi, Brijesh Kumar Singh, Jin Zhou, Keziah Tikno, Anissa Widjaja, Reddemma Sandireddy, Kabilesh Arul, Siti Aishah Binte Abdul Ghani, George Goh Boon Bee, Kiraely Adam Wong, Ho Jia Pei, Shamini Guna Shekeran, Rohit Anthony Sinha, Manvendra K. Singh, Stuart Alexander Cook, Ayako Suzuki, Teegan Reina Lim, Chang-Chuen Cheah, Jue Wang, Rui-Ping Xiao, Xiuqing Zhang, Pierce Kah Hoe Chow, Paul Michael Yen
Summary: The study reveals that hyperhomocysteinemia (HHcy) plays a key role in the pathogenesis of non-alcoholic steatohepatitis (NASH) through homocysteinylation of proteins, leading to autophagy blockage. Vitamin B-12 and folate may serve as a novel first-line therapy for NASH by reducing homocysteine levels and promoting autophagy, thus improving liver histology.
JOURNAL OF HEPATOLOGY
(2022)
Review
Cell Biology
Masum M. Mia, Manvendra K. Singh
Summary: Fibrosis is a defective wound healing process often seen after chronic injury and/or inflammation in various organs. The Hippo signaling pathway plays a crucial role in the pathophysiology of fibrotic diseases, regulating cell proliferation, apoptosis, cell fate decisions, and contributing to the development of organ fibrosis.
Article
Biochemistry & Molecular Biology
Jennifer L. Bailey, David H. Burk, Susan J. Burke, Scott D. Reed, Sujoy Ghosh, Carrie M. Elks
Summary: This study found that adipocyte-specific LAMA4 deletion is protective in an obesogenic environment, even though overall adiposity is increased. Laminin alpha 4 was found to be highly expressed in human and murine adipocytes.
Article
Multidisciplinary Sciences
Wanling Zeng, Hong Chang Tan, Huang Fang Zheng, Amanda Rui Lin Lam, Kok Keong Teo, Chieh Suai Tan, Jean -Paul Kovalik, Sujoy Ghosh, Xiao Hui Xin
Summary: This study aimed to explore the satisfaction of patienst with DKD visiting TCM physicians and its association with socio-economic characteristics. Findings suggest that ethnicity, types of housing, previous TCM experience, and duration of follow-up with TCM physicians may affect the satisfaction scores of DKD patients.
Article
Biochemistry & Molecular Biology
Marc Thibonnier, Sujoy Ghosh
Summary: We propose an innovative approach to drug research and development for miRNA oligonucleotide therapeutics. This model involves collaboration between a biotechnology company, AptamiR Therapeutics, and Centers of Excellence in Academic Institutions. Our focus is on developing safe, effective, and convenient miRNA ONT agents for metabolic disorders and ovarian cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Vetrivel Sengottuvel, Monalisa Hota, Jeongah Oh, Dwight L. Galam, Bernice H. Wong, Markus R. Wenk, Sujoy Ghosh, Federico Torta, David L. Silver
Summary: Patients with autosomal recessive microcephaly 15 caused by deficiency in Mfsd2a, a transporter for LPC in oligodendrocytes, exhibit both microcephaly and hypomyelination. Mfsd2a is specifically expressed in oligodendrocyte precursor cells (OPCs) and plays a critical role in OPC development. Loss of Mfsd2a leads to precocious differentiation of OPCs into immature oligodendrocytes and impaired maturation into myelinating oligodendrocytes, resulting in postnatal brain hypomyelination.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Physiology
Han Fang, Sujoy Ghosh, Landon C. Sims, Kirsten P. Stone, Cristal M. Hill, Denisha Spires, Daria Ilatovskaya, Christopher D. Morrison, Thomas W. Gettys, Krisztian Stadler
Summary: Long-term protein restriction is not beneficial for an aging kidney, as it can lead to renal injury and inflammation. The presence of FGF21 is essential for countering the negative effects of a low-protein diet on the kidney during aging.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)