Article
Veterinary Sciences
Yi Bi, Weijie Feng, Yuxin Kang, Ke Wang, Yuta Yang, Lei Qu, Hong Chen, Xianyong Lan, Chuanying Pan
Summary: The study identified potential mutations in the goat Fec(B) gene and investigated their functions related to fecundity. It found that CNV3 and CNV5 within the Fec(B) gene significantly influenced goat litter size, suggesting they could serve as potential DNA molecular markers for DNA editing and DNA microarray. This study laid a theoretical foundation for the detection of potential fertility-related quantitative trait loci within the goat Fec(B) gene.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Cell Biology
Zhen Wang, Ruolan Wang, Chuanying Pan, Hong Chen, Lei Qu, Lian Wu, Zhengang Guo, Haijing Zhu, Xianyong Lan
Summary: Dynein Axonemal Heavy Chain 1 (DNAH1) encodes proteins that are crucial for the structural support and function of sperm and ova. This study identified several genetic variations within DNAH1 that were significantly associated with litter size in Shaanbei white cashmere goats. These genetic variations can be used as molecular markers for selecting reproductive traits in goats.
Article
Agriculture, Dairy & Animal Science
Peng Wang, Wentao Li, Ziyi Liu, Xiaoyun He, Rong Lan, Yufang Liu, Mingxing Chu
Summary: This study analyzed the association between PPP2R5C and SLC39A5 gene polymorphisms and litter size in Yunshang black goats. The results showed that the polymorphisms PPP2R5C g.65977743C>T and SLC39A5 g.50676693T>C were significantly associated with the litter size of the third parity in Yunshang black goats. Further experiments confirmed that individuals with CC genotype of PPP2R5C and TT genotype of SLC39A5 had higher expression of these genes in goat ovarian tissues. These findings provide new insights into candidate gene selection for marker-assisted selection in goats.
Article
Genetics & Heredity
Tianzeng Song, Yao Tan, Renqing Cuomu, Yacheng Liu, Gui Ba, Langda Suo, Yujiang Wu, Xiaohan Cao, Xianyin Zeng
Summary: In this study, mRNA expression levels and SNP loci of the genes IGF-1, FGF5, and KAP 1.4 were examined in Tibetan cashmere goats. Two new SNP loci were detected in the gene KAP 1.4, while no SNP loci were found in the genes IGF-1 and FGF5. The expression levels of these genes showed significant variation among different counties and ages of cashmere goats.
Article
Agriculture, Dairy & Animal Science
Chang Yue, Wen L. Bai, Yuan Y. Zheng, Tai Y. Hui, Jia M. Sun, Dan Guo, Su L. Guo, Ze Y. Wang
Summary: This study identified a relationship between the polymorphisms of several genes in Liaoning cashmere goats and litter size and cashmere performance. Particularly, specific genotypes at the FecB C94T and ESR C463T loci were associated with higher litter numbers in goats. These findings suggest a significant correlation between FecB and ESR genes and the high fecundity of Liaoning cashmere goats.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Zihong Kang, Yangyang Bai, Xianyong Lan, Haiyu Zhao
Summary: Our study identified a 13-bp indel mutation within goat AKAP12 gene that significantly affects litter size, possibly through its binding capability with miR-181, similar to its role in regulating litter size in pigs.
FRONTIERS IN GENETICS
(2021)
Article
Reproductive Biology
Dwi Wijayanti, Sihuan Zhang, Yuta Yang, Yangyang Bai, Zhanerke Akhatayeva, Chuanying Pan, Haijing Zhu, Lei Qu, Xianyong Lan
Summary: This study found that the expression of the SMAD1 gene in Shaanbei white cashmere goats is significantly associated with litter size. Specifically, the mRNA expression level of SMAD1 in the ovaries of multi-lamb mothers is significantly higher than that of single-lamb mothers. In addition, two InDel loci (18-bp and 7-bp) within the SMAD1 gene are also significantly correlated with litter size.
Article
Agriculture, Dairy & Animal Science
Dwi Wijayanti, Sihuan Zhang, Yangyang Bai, Chuanying Pan, Hong Chen, Lei Qu, Zhengang Guo, Xianyong Lan
Summary: This study reveals the significant role of SMAD2 in goat reproduction and identifies a specific deletion site (P3-Del-12-bp) that is associated with litter size. Heterozygous individuals with this deletion are more likely to produce multiple offspring.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Dwi Wijayanti, Yangyang Bai, Quratulain Hanif, Hong Chen, Haijing Zhu, Lei Qu, Zhengang Guo, Xianyong Lan
Summary: This study identified a significant correlation between a 16-bp deletion within the CLSTN2 gene and first-born litter size in goats.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Reproductive Biology
Zhanerke Akhatayeva, Chunna Cao, Yangming Huang, Qian Zhou, Qingfeng Zhang, Zhengang Guo, Shijie Tan, Ran Li, Xiangpeng Yue, Hongwei Xu, Chuanying Pan, Xianyong Lan
Summary: The study of BMPRIB gene polymorphisms is important for improving reproductive efficiency in sheep. This study assessed the genetic diversity of the 90-bp deletion polymorphism in 52 sheep breeds, examined its linkage to the p.Q249R variant, and analyzed its association with litter size. The results showed that the polymorphism was present in 45 breeds with varying frequencies. The association analysis revealed no significant association between the 90-bp deletion variant and litter size in Australian White, Guiqian semi-fine wool, and SmallTail Han ewes.
Article
Agriculture, Dairy & Animal Science
Xinwei Zhang, Rongrong Yuan, Yangyang Bai, Yuta Yang, Xiaoyue Song, Xianyong Lan, Chuanying Pan
Summary: AKAP13 gene is a candidate gene for regulating female fertility and its 16-bp indel locus is significantly associated with litter size in goats, making it a potential DNA molecular marker for molecular marker-assisted selection in goat breeding.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Xian-feng Wu, Yuan Liu, Jin-shun Zhan, Qin-lou Huang, Wen-yang Li
Summary: This study revealed a novel splice transcript of the CPT1a gene in goats, which is predicted to result in the skipping of exons 6-19. The expression patterns of CPT1a and this novel splice variant, CPT1a-sv1, showed spatial differences and were involved in brain development. These findings provide insights into the function of the CPT1a gene and the molecular mechanism of alternative splicing in goats.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Reproductive Biology
Yong Ruan, Lingang Dai, Jiajing Huang, Meimei Xiao, Jiali Xu, Dongwei An, Jiaqi Chen, Xiang Chen
Summary: This study evaluated the OLR1 sequences and mRNA expression levels in Guizhou white goats to investigate the relationship between gene polymorphisms and litter size. The results showed that OLR1 expression levels were highest in the uterus of mothers with multiple kids and highest in the ovaries of mothers with single kids. Sequencing results identified three significant SNPs associated with litter size. One of the SNPs resulted in a non-synonymous mutation in the amino acid residue of the OLR1 protein. This study confirms the role of OLR1 in goat kidding traits and provides new insights into its regulatory mechanism on litter size.
Article
Zoology
Fulin Zhao, Chen Liang, Lin Tao, Yanting Jiang, Yina Ouyang, Qionghua Hong, Mingxing Chu
Summary: This study aimed to analyze the association between single nucleotide polymorphisms (SNPs) in the FGFR1 and EBP41L5 genes and kidding performance of goats. SNP typing technology was applied to genotype the SNPs and the genetic characteristics of different goat populations were explored. The association between FGFR1 and EBP41L5 and kidding performance was analyzed, and it was found that EPB41L5 gene loci were suitable as molecular markers for litter size and litter weight at weaning in certain goat populations.
PAKISTAN JOURNAL OF ZOOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
M. Silpa, T. Naicy, T. Aravindakshan, G. Radhika, J. Joan, S. Jinty
Summary: This study investigated the role of SIRT3 gene in goat fertility, finding higher expression of SIRT3 in Malabari goats, polymorphism in exon 7, and significant association between SIRT3 genotypes and litter size, suggesting SIRT3 as a potential candidate gene for genetic improvement in goats.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.