Article
Microbiology
Nathalie Renard, Soizic Daniel, Nadege Cayet, Matthieu Pecquet, Frederique Raymond, Sylvie Pons, Julien Lupo, Carole Tourneur, Catherine Pretis, Guillaume Gerez, Patrick Blasco, Maxime Combe, Imen Canova, Mylene Lesenechal, Franck Berthier
Summary: This study validates the high specificity and sensitivity of Vidas SARS-CoV-2 IgM and Vidas SARS-CoV-2 IgG, suitable for reliably detecting past acute SARS-CoV-2 infections. The assays showed a 100% positive percent agreement with SARS-CoV-2 PCR at >= 16 days (Vidas SARS-CoV-2 IgM) and >= 32 days (Vidas SARS-CoV-2 IgG) of symptom onset.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Review
Virology
Georg Bauer
Summary: Analysis of 16 recent reports on the serological response to SARS-CoV-2 showed high variability, with IgM and IgG responses unable to clearly distinguish early, intermediate, and past infections. A proposed model explains this variability and suggests the potential use of avidity determination for various diagnostic and epidemiological applications, including COVID-19 diagnosis and vaccination program control.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Infectious Diseases
Valentina Pecoraro, Tiziana Cassetti, Marisa Meacci, Raffaele Gargiulo, Maria Rosaria Capobianchi, Cristina Mussini, Elena Vecchi, Monica Pecorari, Carlo Gagliotti, Tommaso Trenti, Mario Sarti
Summary: In this study, the accuracy of CLIA and two LFIA methods in detecting IgG and IgM antibodies to SARS-CoV-2 virus was evaluated. CLIA showed higher sensitivity and specificity for IgG and IgM detection compared to LFIA methods, especially in patients.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Microbiology
Justyna Adamczuk, Piotr Czupryna, Justyna Dunaj-Malyszko, Ewelina Kruszewska, Slawomir Pancewicz, Karol Kaminski, Karol Borawski, Sambor Grygorczuk, Anna Moniuszko-Malinowska
Summary: The study aimed to assess the presence of anti-SARS-CoV-2 antibodies in COVID-19 convalescents and investigate their production in relation to disease severity, sex, vaccination, and correlation with inflammatory parameters. The results showed that immunological response could last over 6 months, with significantly higher antibody titers in severe cases and vaccinated patients. The immunological response was not dependent on sex. There was also a significant correlation between antibody production and the degree of inflammation.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Maria Denaro, Elisa Ferro, Giuseppe Barrano, Salvatore Meli, Mariangela Busacca, Damiano Corallo, Alessia Capici, Alessandra Zisa, Luana Cucuzza, Sandra Gradante, Marialuisa Occhipinti, Paola Santalucia, Raffaele Elia, Angelo Aliquo, Daniele Tibullo, Carmelo Fidone, Vincenzo Bramanti
Summary: The study found that the trend of SARS-CoV-2 variants in Ragusa area is comparable to the Sicily region based on genomic sequencing and immune monitoring of 600 samples. Although no correlation was found between variants and clinical symptoms, antibody levels were positively correlated with an increase in the number of symptoms. Furthermore, SARS-CoV-2 infection induced a significantly higher enhancement in antibody titers compared to vaccine administration. Therefore, the evaluation of anti-N IgG levels could be used as an early marker to identify asymptomatic individuals in the post-pandemic period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
John S. Cognetti, Benjamin L. Miller
Summary: Researchers have developed a rapid diagnostic system utilizing photon sensor technology to detect biomarkers in the immune response post-vaccination, aiding in understanding vaccine mechanisms and individual responses to vaccines.
Article
Multidisciplinary Sciences
Allison N. Grossberg, Lilia A. Koza, Aurelie Ledreux, Chad Prusmack, Hari Krishnan Krishnamurthy, Vasanth Jayaraman, Ann-Charlotte Granholm, Daniel A. Linseman
Summary: This study presents results from a multiplex serology test for assessing COVID-19 antibody responses, showing distinct dynamics of IgG, IgM, and IgA responses in patients with different disease severity. It suggests that antibody responses to SARS-CoV-2 may serve as important biomarkers for assessing the risk of viral transmission.
NATURE COMMUNICATIONS
(2021)
Article
Infectious Diseases
Maria Simon Sacristan, Ana Collazos-Blanco, Maria Isabel Zamora Cintas, Alicia Serrano Garcia, Carmen Ybarra de Villavicencio, Maria Mateo Maestre
Summary: Our study demonstrates that chemiluminescence immunoassays perform better than immunochromatographic tests in diagnosing COVID-19 patients, indicating their potential in the diagnosis of SARS-CoV-2 infections.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Emmanuelle Billon-Denis, Audrey Ferrier-Rembert, Annabelle Garnier, Laurence Cheutin, Clarisse Vigne, Emilie Tessier, Jessica Denis, Cyril Badaut, Clemence Rougeaux, Anne Depeille Wuille, Hawa Timera, Laet itia Boutin, Isabelle Drouet, Noemie Verguet, Flora Nolent, Olivier Gorge, Olivier Ferraris, Jean-Nicolas Tournier
Summary: Two coworkers were infected by the same SARS-CoV-2 strain with different immunological outcomes. One had low and rapidly decreasing neutralizing antibody titers, while the other had high titers. Host individual factors may be the main drivers of the varying immune responses.
Article
Biophysics
Michailia Angelopoulou, Eleni Makarona, Alexandros Salapatas, Konstantinos Misiakos, Evgenia Synolaki, Anastasios Ioannidis, Stylianos Chatzipanagiotou, Mikael A. Ritvos, Arja Pasternack, Olli Ritvos, Panagiota S. Petrou, Sotirios E. Kakabakos
Summary: Silicon photonic probes based on broad-band Mach-Zehnder interferometry were explored as directly immersible immunosensors, eliminating the need for microfluidics and pumps. The probes demonstrated analytical potential through detection of antibodies against SARS-CoV-2, showing promise for rapid point-of-care testing.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Immunology
Sophia Hober, Cecilia Hellstrom, Jennie Olofsson, Eni Andersson, Sofia Bergstrom, August Jernbom Falk, Shaghayegh Bayati, Sara Mravinacova, Ronald Sjoberg, Jamil Yousef, Lovisa Skoglund, Sara Kanje, Anna Berling, Anne-Sophie Svensson, Gabriella Jensen, Henric Enstedt, Delaram Afshari, Lan Lan Xu, Martin Zwahlen, Kalle von Feilitzen, Leo Hanke, Ben Murrell, Gerald McInerney, Gunilla B. Karlsson Hedestam, Christofer Lendel, Robert G. Roth, Ingmar Skoog, Elisabet Svenungsson, Tomas Olsson, Anna Fogdell-Hahn, Ylva Lindroth, Maria Lundgren, Kimia T. Maleki, Nina Lagerqvist, Jonas Klingstrom, Rui Da Silva Rodrigues, Sandra Muschiol, Gordana Bogdanovic, Laila Sara Arroyo Muhr, Carina Eklund, Camilla Lagheden, Joakim Dillner, Asa Sivertsson, Sebastian Havervall, Charlotte Thalin, Hanna Tegel, Elisa Pin, Anna Manberg, My Hedhammar, Peter Nilsson
Summary: A high-throughput multiplex bead-based serological assay was developed and validated, using three selected antigens with high sensitivity and specificity. The best assay performance was achieved with a combination of two antigens, providing a sensitivity of 99.7% and a specificity of 100%.
CLINICAL & TRANSLATIONAL IMMUNOLOGY
(2021)
Article
Chemistry, Analytical
Bin Zhao, Weijing Wang, Nantao Li, Teresa Garcia-Lezana, Congnyu Che, Xiaojing Wang, Bojan Losic, Augusto Villanueva, Brian T. Cunningham
Summary: The study introduces a novel nucleic acid toehold probe-based photonic resonator absorption microscopy (PRAM) assay for rapid and quantitative analysis of various liver cancer-associated exosomal snRNA. This method provides highly sensitive and selective detection of three snRNA targets within 30 minutes, operating at room temperature without enzymatic amplification and with a simple workflow.
Article
Multidisciplinary Sciences
Kamonthip Rungrojcharoenkit, Rungarun Suthangkornkul, Darunee Utennam, Darunee Buddhari, Soontorn Pinpaiboon, Duangrat Mongkolsirichaikul, Stefan Fernandez, Anthony R. Jones, Thomas S. Cotrone, Taweewun Hunsawong
Summary: RT-PCR is the gold standard method for diagnosing COVID-19, but additional diagnostic tests are needed. We developed in-house anti-RBD IgG and IgA ELISAs for screening and identifying SARS-CoV-2 infection. Our in-house ELISAs showed high sensitivity and specificity, and were comparable to other commercial ELISAs.
Article
Microbiology
Lene H. Harritshoj, Mikkel Gybel-Brask, Shoaib Afzal, Pia R. Kamstrup, Charlotte S. Jorgensen, Marianne Kragh Thomsen, Linda Hilsted, Lennart Friis-Hansen, Pal B. Szecsi, Lise Pedersen, Lene Nielsen, Cecilie B. Hansen, Peter Garred, Trine-Line Korsholm, Susan Mikkelsen, Kirstine O. Nielsen, Bjarne K. Moller, Anne T. Hansen, Kasper K. Iversen, Pernille B. Nielsen, Rasmus B. Hasselbalch, Kamille Fogh, Jakob B. Norsk, Jonas Henrik Kristensen, Kristian Schonning, Nikolai S. Kirkby, Alex C. Y. Nielsen, Lone H. Landsy, Mette Loftager, Dorte K. Holm, Anna C. Nilsson, Susanne G. Saekmose, Birgitte Grum-Schwensen, Bitten Aagaard, Thoger G. Jensen, Dorte M. Nielsen, Henrik Ullum, Ram B. Dessau
Summary: This study evaluated the diagnostic accuracy of 15 commercial and one in-house anti-SARS-CoV-2 assays in Danish laboratories. Most total antibody and IgG assays showed high sensitivity and specificity, with some overlap in confidence intervals. IgM results were more variable, with one assay demonstrating higher sensitivity and specificity.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Weiliang Liu, Desheng Chen, Hongru Pian, Fengxia Su, Hui Wang, Pengbo Zhang, Zhengping Li
Summary: In this study, a novel one-by-one single-molecule counting method for digital quantification of SARS-Cov-2 RNA is reported. The method utilizes a fluorescent micromotor functionalized with peptide nucleic acids (PNAs) to specifically capture the target RNA molecules, enabling accurate counting and discrimination of mutations. This method demonstrates great potential for clinical diagnostics and virus traceability survey.
Review
Chemistry, Analytical
Yanyu Xiong, Nantao Li, Congnyu Che, Weijing Wang, Priyash Barya, Weinan Liu, Leyang Liu, Xiaojing Wang, Shaoxiong Wu, Huan Hu, Brian T. Cunningham
Summary: The development of nanostructured materials in biosensor research has made significant progress in amplifying the interaction between light and biological matter, providing new solutions for enhancing contrast and signal-to-noise ratio in microscopy.
Article
Chemistry, Analytical
Bin Zhao, Weijing Wang, Nantao Li, Teresa Garcia-Lezana, Congnyu Che, Xiaojing Wang, Bojan Losic, Augusto Villanueva, Brian T. Cunningham
Summary: The study introduces a novel nucleic acid toehold probe-based photonic resonator absorption microscopy (PRAM) assay for rapid and quantitative analysis of various liver cancer-associated exosomal snRNA. This method provides highly sensitive and selective detection of three snRNA targets within 30 minutes, operating at room temperature without enzymatic amplification and with a simple workflow.
Article
Chemistry, Multidisciplinary
Neha Chauhan, Yanyu Xiong, Shaokang Ren, Abhisek Dwivedy, Nicholas Magazine, Lifeng Zhou, Xiaohe Jin, Tianyi Zhang, Brian T. Cunningham, Sherwood Yao, Weishan Huang, Xing Wang
Summary: We propose a net-shaped DNA nanostructure design strategy for selective recognition and capture of intact SARS-CoV-2 virions, enabling the development of a rapid and inexpensive COVID-19 test. Furthermore, this strategy can be customized for tackling other viruses.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Multidisciplinary Sciences
Yanyu Xiong, Qinglan Huang, Taylor D. Canady, Priyash Barya, Shengyan Liu, Opeyemi H. Arogundade, Caitlin M. Race, Congnyu Che, Xiaojing Wang, Lifeng Zhou, Xing Wang, Manish Kohli, Andrew M. Smith, Brian T. Cunningham
Summary: The authors report a method for achieving nearly 3000-fold signal enhancement using a photonic crystal surface. By enhancing excitation, improving quantum efficiency, and suppressing blinking, the approach enables high signal-to-noise ratio and single emitter sensitivity. The study also demonstrates the application of this method in miRNA biomarker sensing.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Xiaojing Wang, Skye Shepherd, Nantao Li, Congnyu Che, Tingjie Song, Yanyu Xiong, Isabella Rose Palm, Bin Zhao, Manish Kohli, Utkan Demirci, Yi Lu, Brian T. T. Cunningham
Summary: In this study, an efficient target recycling amplification process (TRAP) using photonic resonator absorption microscopy was developed for the digital detection of exosomal microRNAs (miRNAs). The TRAP approach demonstrated high sensitivity, robust selectivity, and a broad dynamic range, making it ideal for quantifying miRNA biomarkers in low concentrations or sample volume. Compared to traditional qRT-PCR, TRAP showed similar accuracy in profiling exosomal miRNAs derived from cancer cells, but with significantly enhanced detection limits for miRNA-375 and miRNA-21.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biophysics
Leyang Liu, Joseph Tibbs, Nantao Li, Amanda Bacon, Skye Shepherd, Hankeun Lee, Neha Chauhan, Utkan Demirci, Xing Wang, Brian T. Cunningham
Summary: In this paper, a compact Photonic Resonator Interferometric Scattering Microscope (PRISM) designed for point-of-use environments and applications is presented. It allows label-free detection and digital counting of nanometer-scaled objects such as nanoparticles, viruses, extracellular vesicles, and protein molecules. The instrument incorporates two innovative elements to facilitate operation in ordinary laboratory environments and achieves stable image contrast over time and spatial position through an automated focusing module.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Priyash Barya, Yanyu Xiong, Skye Shepherd, Rohit Gupta, Lucas D. Akin, Joseph Tibbs, Hankeun Lee, Srikanth Singamaneni, Brian T. Cunningham
Summary: Assays using fluorophores are common in life science research and diagnostics. However, weak emission intensity limits the detection limits, requiring a large number of labeled target molecules to improve signal-to-noise ratio. This study demonstrates that the synergistic coupling of plasmonic and photonic modes can significantly enhance the emission from fluorophores, resulting in a 52-fold improvement in signal intensity. The method is successfully applied to a sandwich immunoassay for human interleukin-6, achieving a detection limit three orders of magnitude lower than standard immunoassays.
Review
Chemistry, Analytical
Yanyu Xiong, Skye Shepherd, Joseph Tibbs, Amanda Bacon, Weinan Liu, Lucas D. Akin, Takhmina Ayupova, Seemesh Bhaskar, Brian T. Cunningham
Summary: Nanoscale fluorescence emitters are efficient in measuring biomolecular interactions but limited in applications requiring single-unit observations due to various challenges. Photonic crystal structures offer solutions for fluorescence enhancement by optimizing numerical aperture objectives, reducing fluorescence intermittency, and improving photon collection efficiency. This review provides an overview of photonic crystals, their design, fabrication, and sensing principles, as well as recent applications in biosensors for nucleic acids, proteins, and steroids. The review also discusses current challenges and future prospects of fluorescence enhancement with photonic-plasmonics coupling in point-of-care biosensing and monitoring analytes of biological and environmental relevance.
Review
Chemistry, Analytical
Amanda Bacon, Weijing Wang, Hankeun Lee, Saurabh Umrao, Prima Dewi Sinawang, Demir Akin, Kodchakorn Khemtonglang, Anqi Tan, Sabina Hirshfield, Utkan Demirci, Xing Wang, Brian T. Cunningham
Summary: The ability for individuals to self-test for HIV is crucial in preventing transmission, especially when combined with other biomedical prevention methods like PrEP. In this article, we review recent advancements in HIV self-testing and sampling methods, and explore the potential impact of new materials and techniques developed for more effective point-of-care SARS-CoV-2 diagnostics. We discuss the limitations of current HIV self-testing technologies and the need for improvements in sensitivity, speed, simplicity, and cost to enhance diagnostic accuracy and accessibility. We also examine potential paths towards the next generation of HIV self-testing through sample collection materials, biosensing assays, and miniaturized instruments, as well as the implications for self-monitoring HIV viral load and other infectious diseases.
Proceedings Paper
Engineering, Electrical & Electronic
Yanyu Xiong, Qinglan Huang, Taylor D. Canady, Priyash Barya, Shengyan Liu, Opeyemi H. Arogundade, Caitlin M. Race, Congnyu Che, Xiaojing Wang, Lifeng Zhou, Anh Igarashi, Xing Wang, Manish Kohli, Andrew M. Smith, Brian T. Cunningham
Summary: In this study, the signal of nanoscale quantum emitters was significantly boosted using the multiplicative effects of amplified excitation, highly directed extraction, Purcell enhancement, and blinking suppression on a photonic crystal surface. The platform achieved single quantum dot sensitivity with high signal-to-noise ratio, and ultrasensitive detection with a wide linear dose-response range.
Article
Chemistry, Analytical
Aaron M. Jankelow, Hankeun Lee, Weijing Wang, Trung-Hieu Hoang, Amanda Bacon, Fu Sun, Seol Chae, Victoria Kindratenko, Katherine Koprowski, Robert A. Stavins, Dylann D. Ceriani, Zachary W. Engelder, William P. King, Minh N. Do, Rashid Bashir, Enrique Valera, Brian T. Cunningham
Summary: In this study, we report an integrated system for rapid detection of viral pathogens. The system can perform sample processing and nucleic acid amplification in 20 minutes and can analyze the results using a smartphone. The system offers the advantages of low cost, high accuracy, and simplicity in operation while achieving rapid detection.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
