Fast and Sensitive Bacteria Detection by Boronic Acid Modified Fluorescent Dendrimer

This study reports a novel, fast, easy, and sensitive detection method for bacteria which is urgently needed to diagnose infections in their early stages. Our work presents a complex of poly(amidoamine) dendrimer modified by phenylboronic acid and labeled by a fluorescent dansyl group (Dan-B8.5-PAMAM). Our system detects bacteria in 20 min with a sensitivity of approximately 10(4) colony-forming units (CFU)center dot mL(-1). Moreover, it does not require any peculiar technical skills or expensive materials. The driving force for bacteria recognition is the binding between terminal phenylboronic acids on the probe and bacteria's surface glycolipids, rather than electrostatic interactions. The aggregation caused by such binding reduces fluorescence. Even though our recognition method does not distinguish between live or dead bacteria, it shows selective antibacterial activity towards Gram-negative bacteria. This study may potentially contribute a new method for the convenient detection and killing of bacteria.

Automated summary

This study introduces a novel and sensitive bacteria detection method using a poly(amidoamine) dendrimer modified by phenylboronic acid, which can detect bacteria quickly and selectively target Gram-negative bacteria. The detection system does not require specialized technical skills or expensive materials, providing a convenient and potentially effective method for bacteria detection and elimination.