Article
Biotechnology & Applied Microbiology
Marco Chiappero, Franco Berruti, Silvia Fiore
Summary: This study investigated the biomethane potential of wine lees through mesophilic batch tests and evaluated the influence of wood-based biochar addition. The results showed that wine lees are a highly attractive anaerobic digestion substrate, but careful operation is required.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Muhammad Shahbaz, Muhammad Ammar, Shahid Hussain, Toheed Ahmed, Waqar Muhammad Ashraf, Ali Raza, Ahsan Amjad, Dexun Zou
Summary: This study investigated the impact of OLR on the AD of MSW with a high C/N ratio and found that OLR significantly affects the performance and stability of the digester. The highest biogas and methane yield were observed at OLR of 10 gVS L-1, while a rapid decrease in performance and stability was found at OLR of 15 and 20 gVS L-1.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Samson Nnaemeka Ugwu, Kevin Harding, Christopher Chintua Enweremadu
Summary: This study conducted a comparative assessment of the potential environmental impacts of biogas production from enhanced anaerobic digestion using different enhancement options for agro-industrial wastes. The results revealed that the combined enhancement option (co-digestion + PPy/Fe3O4 NPs) had the least global warming potential.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Engineering, Chemical
Mawaheb Mouftahi, Nawel Tlili, Nejib Hidouri, Pietro Bartocci, Khalideh Al Bkoor Alrawashdeh, Eid Gul, Federica Liberti, Francesco Fantozzi
Summary: The study in Tunisia focuses on evaluating the biogas and bio-methane yield from the co-digestion of three available bio-wastes in the southern regions, which showed a high methane production and reduced the amount of wastes to dispose of. The experimental work under specific conditions resulted in a high yield of net methane and net biogas, with a significant reduction in volatile solids and a hydraulic retention time of 40 days.
Article
Agricultural Engineering
Hao Jiang, Fan Wu, Yuchang Wang, Lu Feng, Hongjun Zhou, Yeqing Li
Summary: In-situ hydrogen biomethanation is a promising technology for upgrading biogas quality, and investigating its characteristics under different conditions is crucial for its effectiveness. The experiments showed that hydrogenotrophic methanogenesis performed better at thermophilic conditions, with the highest methane content obtained when H2 and CO2 were fed at a 4:1 ratio with Methanothermobacter as the dominant genus.
BIORESOURCE TECHNOLOGY
(2021)
Article
Thermodynamics
Kavosh Karami, Keikhosro Karimi, Safoora Mirmohamadsadeghi, Rajeev Kumar
Summary: This study investigated the effects of biological pretreatment on biogas production from lignocellulosic biomass and studied the combination effect with other pretreatment methods. The results showed that aerobic digestion was the only pretreatment method that could individually improve methane yield from pinewood, but combining aerobic digestion with mild thermochemical pretreatment could achieve even higher methane yield.
Article
Agricultural Engineering
Rokaya Bouaita, Kerroum Derbal, Antonio Panico, Floriana Iasimone, Ludovico Pontoni, Massimiliano Fabbricino, Francesco Pirozzi
Summary: A large amount of orange peel waste is produced every year due to high orange production worldwide. This waste is often dumped without proper treatment, causing environmental issues. Anaerobic digestion is suggested as an effective treatment for orange peel waste, but the high content of limonene can inhibit the biological activity. Anaerobic co-digestion with organic fraction of municipal solid waste can reduce the limonene concentration. The limonene content is directly related to the organic loading rate, and the maximum stable organic loading rate was found to be 2 g(VS)(L-1.day(-1)).
BIOMASS & BIOENERGY
(2022)
Article
Chemistry, Physical
Ikirsha Jokhio, Sundus Saeed Qureshi, Abdul Razzaq Sahito, Mehr-un-Nisa Qureshi, Imtiaz Ahmed Lashari, N. M. Mubarak, Manoj Tripathi, Amal M. Al-Mohaimeed, Mohamed S. Elshikh
Summary: The yield of bio-methane and bio-hydrogen was enhanced by co-digesting banana plant waste (BPW) and sewage sludge (SS). Acclimatisation of the inoculum SS was performed in a continuous stirred tank reactor (CSTR), and its various parameters were analyzed daily. Six different BPW to SS ratios were optimized and incubated in a manual methane potential test setup (MMPTS) for 40 days, with R4 (60% BPW and 40% SS) showing the highest yields. Furthermore, the best achieved ratio of BPW and SS was treated with different doses of NaOH as an alkaline pre-treatment, and the highest bio-methane yield was observed with dose 2 (0.75 mol NaOH) at 620.8 NmL/day. The study demonstrates the promising potential of co-digestion of BPW and SS for enhanced bio-methane and bio-hydrogen production.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Agricultural Engineering
Monika Yadav, Vivekanand Vivekanand
Summary: In this study, chitinolytic bacteria were used for bioaugmentation of fungal pretreated agricultural residues to enhance biogas production. Batch and continuous experiments showed that the addition of chitinolytic bacteria to wheat and pearl millet straw pretreated with Chaetomium globosporum resulted in increased biogas yield. Microbial community analysis revealed higher abundance of methanogens in reactors running with pretreated wheat straw, mainly consisting of Firmicutes, Bacteroidetes, Proteobacteria phyla.
BIORESOURCE TECHNOLOGY
(2021)
Article
Environmental Sciences
Aiban Abdulhakim Saeed Ghaleb, Shamsul Rahman Mohamed Kutty, Gasim Hayder Ahmed Salih, Ahmad Hussaini Jagaba, Azmatullah Noor, Vicky Kumar, Najib Mohammed Yahya Almahbashi, Anwar Ameen Hezam Saeed, Baker Nasser Saleh Al-dhawi
Summary: Effective bio-waste management can reduce global pollution caused by man-made organic waste and promote the development of the circular economy. The study determined the optimal carbon/nitrogen ratio and co-substrate/inoculum ratio for the co-digestion process, finding that sugarcane bagasse plays a crucial role in enhancing biogas production.
Article
Environmental Sciences
Cheng Yu, Bi Dongsu, Zhang Tao, Jiang Xintong, Chen Ming, Wang Siqi, Shen Zheng, Zhang Yalei
Summary: Bioplastic bags have the potential to replace traditional petroleum-based plastic bags as they can be anaerobically digested with food waste to produce renewable energy.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Agricultural Engineering
Pan Wang, Xinzi Wang, Xiteng Chen, Lianhai Ren
Summary: This study found that adding bentonite during anaerobic digestion of food waste can increase methane production and effectively reduce the abundance of antibiotic resistance genes (ARGs) in biogas residue. Bentonite and temperature were identified as major factors influencing the abundance of ARGs.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Xiaoshuang Zou, Yiran Wang, Yongheng Dai, Shaoqi Zhou, Bin Wang, Yancheng Li, Jiang Li
Summary: This study investigated the anaerobic co-digestion of excess sludge and different varieties of rice straw. The results showed that the addition of glutinous rice straw (GRS) had the highest hydrolysis and methanogenesis rates, and it promoted the degradation of lignocellulose. The digestion process was influenced by the abundance of certain bacteria. Additionally, the addition of GRS increased the nitrogen concentration in biogas residue and the phosphorus concentration in biogas slurry, but hindered the subsequent sludge dehydration.
BIORESOURCE TECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Miriam Cerrillo, Laura Burgos, Beatriz Ruiz, Raquel Barrena, Javier Moral-Vico, Xavier Font, Antoni Sanchez, August Bonmati
Summary: The addition of zero-valent iron nanoparticles (nZVI) had different effects on methane production during anaerobic digestion of pig slurry under mesophilic and thermophilic conditions. Batch experiments showed inhibition of methane production at all tested dosages in mesophilic conditions, while boosting methane production at the lowest dosage in thermophilic conditions. Continuous operation with nZVI addition resulted in increased methane content in biogas, with a significant increase in methane production rate compared to the control in both temperature ranges. The oxidation state of nZVI exhibited immediate oxidation upon contact with substrate and gradual oxidation during reactor operation.
Article
Agricultural Engineering
Matteo Palu, Maria Peprah, Panagiotis Tsapekos, Panagiotis Kougias, Stefano Campanaro, Irini Angelidaki, Laura Treu
Summary: This study investigates the effects of bioaugmentation on the co-digestion of cheese whey and manure in a continuous stirred tank reactor under in-situ biomethanation operations. The addition of Methanoculleus bourgensis in the mesophilic reactor resulted in a stable biomethanation process and increased methane production rate. On the other hand, the addition of Methanothermobacter thermautotrophicus in the thermophilic reactor did not improve biogas upgrading performance.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agronomy
Agnes Szepesi, Laszlo Bakacsy, Henrietta Kovacs, Arpad Szilagyi, Zoltan Marton Kohler
Summary: This study explores the role of CuAO in different tomato genotypes at different stages of seedling development. The results indicate that there is genotype dependence in the degradation of Put, and inhibition of CuAO activity leads to a long-term shift in PA catabolism by altering the activity of PAO.
Article
Biotechnology & Applied Microbiology
Etelka Kovacs, Csilla Szucs, Attila Farkas, Mark Szuhaj, Gergely Maroti, Zoltan Bagi, Gabor Rakhely, Kornel L. Kovacs
Summary: This study investigated the decomposition of lignocellulosic plant biomass by four filamentous fungi. It was found that the Penicillium aurantiogriseum strain exhibited excellent hydrolytic enzyme production and generated the highest biogas yield from partially degraded substrates. Corn stover was the most suitable substrate for biomass decomposition and biogas production.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Sandor Vaczi, Lilla Barna, Krisztian Laczi, Ferenc Tomosi, Gabor Rakhely, Botond Penke, Livia Fulop, Ferenc Bogar, Tamas Janaky, Maria A. Deli, Zsofia Mezei
Summary: S1R ligands regulate cellular functions and local blood circulation by influencing arachidonic acid metabolism. Sub-chronic, in vivo administration of S1R ligands in streptozotocin-induced diabetic rats had different effects, some of which were protective and some promoted the recovery of diabetes.
Article
Biotechnology & Applied Microbiology
Gergely Erno Lakatos, Karolina Ranglova, Daniela Barcenas-Perez, Tomas Grivalsky, Joao Camara Manoel, Mykola Mylenko, Jose Cheel, Jozsef Nyari, Roland Wirth, Kornel L. Kovacs, Jiri Kopecky, Linda Nedbalova, Jiri Masojidek
Summary: Three cultivation regimes were tested for cold-adapted cultures of Monoraphidium in an outdoor pond: sunlight cultivation, combination of sunlight and supplementary illumination, and nitrogen depletion using both light sources. The highest productivity was achieved when sunlight was combined with supplementary illumination, with a specific growth rate of 0.191 d-1. Nitrogen depletion stress resulted in the highest total fatty acid content of 20.29% DW, with oleic acid accounting for 54% of the total fatty acid content. The cultivation under sunlight in the first trial yielded a significant amount of lutein, 26.39 mg lutein g-1 DW. In the second trial, 236 mLN g-1oTS of methane was generated from the harvested and fermented biomass.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Ecology
Roland Wirth, Zoltan Bagi, Prateek Shetty, Mark Szuhaj, Teur Teur Sally Cheung, Kornel L. Kovacs, Gergely Maroti
Summary: Multi-omics analysis was used to study inter-kingdom interactions in biogas-producing microbial communities. The microbiomes of three biogas digesters were analysed using a metagenomics framework, revealing the relationship between methanogenic communities and their bacterial partners. The study identified high-quality metagenome-assembled genomes and found that the abundance of microorganisms was independent of their activity. The most active metabolic pathway was found to be methanogenesis.
Article
Biochemistry & Molecular Biology
Alajos Berczi, Zsuzsanna Marton, Krisztina Laskay, Andras Toth, Gabor Rakhely, Agnes Duzs, Krisztina Sebok-Nagy, Tibor Pali, Laszlo Zimanyi
Summary: Cytochrome b561 proteins are integral membrane proteins that have ascorbate reducibility and trans-membrane electron transferring capability. They have been found in various animal and plant phyla and are localized in different membranes. Two homologous proteins in humans and rodents are thought to be involved in cancer pathology. This paper presents the physical-chemical properties of the homologues in mice and humans, providing insights into the CYB561 protein family.
Article
Biochemistry & Molecular Biology
Gyorgyi Varadi, Zoltan Kele, Andras Czajlik, Attila Borics, Gabor Bende, Csaba Papp, Gabor Rakhely, Gabor K. Toth, Gyula Batta, Laszlo Galgoczy
Summary: Due to the fast spread of drug resistance, only a limited number of drugs can be used to treat fungal infections. Therefore, there is an urgent need to develop new antifungal treatment strategies. The NFAP2 protein secreted by the mold Neosartorya shows promise as a template for future antifungal drug design, but it is difficult to determine its disulfide linkage pattern using traditional mass spectrometry-based methods.
Article
Multidisciplinary Sciences
Balazs Kakuk, Akos Dormo, Zsolt Csabai, Gabor Kemenesi, Jiri Holoubek, Daniel Ruzek, Istvan Prazsak, Virag eva Dani, Bela Denes, Gabor Torma, Ferenc Jakab, Gabor E. Toth, Fanni V. Foldes, Brigitta Zana, Zsofia Lanszki, Akos Harangozo, Adam Fulop, Gabor Gulyas, Mate Mizik, Andras Attila Kiss, Dora Tombacz, Zsolt Boldogkoi
Summary: The recent Monkeypox outbreak highlighted the importance of studying the basic biology of orthopoxviruses. However, there has been no investigation into the transcriptome of the causative agent using either short-read or long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap and allows for the comprehensive characterization of the monkeypox virus transcriptomic architecture and the potential annotation of novel host transcripts. Additionally, this study will provide insights into gene expression changes in both the virus and host cells during infection, leading to a deeper understanding of the alterations caused by viral infection at the transcriptome level.
Article
Pharmacology & Pharmacy
Balint Racz, Annamaria Kincses, Krisztian Laczi, Gabor Rakhely, Enrique Dominguez-alvarez, Gabriella Spengler
Summary: Recently, the anticancer activity of symmetrical selenoesters was investigated in sensitive and resistant human colon adenocarcinoma cells. The compounds showed synergistic interaction with doxorubicin and exhibited ABCB1 inhibitory and apoptosis-inducing effects. Transcriptome analysis revealed that symmetrical selenoesters may influence the redox state of cells and interfere with metastasis formation.
Review
Biology
Zoltan Marton Kohler, Agnes Szepesi
Summary: L-aminoguanidine (AG) is widely used as an inhibitor in studying plant abiotic stress responses, but its exact mode of action is not well understood. It has been used as a specific diamine oxidase inhibitor and for reducing nitric oxide production. Recent studies suggest its antiglycation effect, but this remains unclear in plants. This review provides a summary of current knowledge about the different targets of AG in plants and recommends using it as a modulator of polyamine-related mechanisms.
Article
Biotechnology & Applied Microbiology
Mark Szuhaj, Balazs Kakuk, Roland Wirth, Gabor Rakhely, Kornel Lajos Kovacs, Zoltan Bagi
Summary: This study investigated the biomethane formation from 4 H-2 + CO2 by two methanogens, Methanocaldococcus fervens and Methanobacterium thermophilum. The research aimed to understand the regulation of biomethane biosynthesis by H-2. It was found that the autotrophic methanogen M. fervens could be easily controlled by H-2/CO2, while the heterotrophic methanogen M. thermophilum was less sensitive to H-2/CO2, suggesting the importance of the biomethane-generating methanogenic community in renewable excess electricity conversion and storage systems.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nora Zsindely, Gabor Nagy, Fruzsina Siagi, Anita Farkas, Laszlo Bodai
Summary: Huntington's disease (HD) is a fatal neurodegenerative disorder caused by a repeat expansion in the Huntingtin gene. In a Drosophila model of HD, dysregulation of miRNA and mRNA expression was observed. Dysregulated miRNAs were found to impact similar molecular pathways as dysregulated mRNAs. Overexpression of certain misregulated miRNAs enhanced or ameliorated HD phenotypes in the model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Agnes Szepesi, Laszlo Bakacsy, Attila Feher, Henrietta Kovacs, Peter Palfi, Peter Poor, Reka Szollosi, Orsolya Kinga Gondor, Tibor Janda, Gabriella Szalai, Christian Lindermayr, Laszlo Szabados, Laura Zsigmond
Summary: Polyamine catabolism mediated by amine oxidases is important for regulating polyamine homeostasis and related mechanisms during salt stress. The effects of L-aminoguanidine (AG), an inhibitor of copper amine oxidases, on tomato roots exposed to short-term salt stress were studied. AG treatment alleviated the increase in reactive oxygen and nitrogen species caused by salt stress, suggesting its possible antioxidant effect.
Review
Biotechnology & Applied Microbiology
Noemi N. Horvath-Gonczi, Zoltan Bagi, Mark Szuhaj, Gabor Rakhely, Kornel L. Kovacs
Summary: Bioelectrochemical systems (BESs) have great potential in renewable energy production technologies, with the ability to generate electricity via Microbial Fuel Cell (MFC) or synthesize valuable commodities through Microbial Electrolysis Cells (MECs). Although industrial-scale operation still faces difficulties, various reactor configurations and operational protocols are rapidly increasing. This article reviews recent BES literature, focusing on electrosynthesis and promising reactor configurations, while attempting to clarify the numerous definitions proposed for BESs. The main components of BESs are highlighted, and useful and generally applicable operational parameters are collected for comparative studies.
FERMENTATION-BASEL
(2023)
