Article
Multidisciplinary Sciences
Qiang Zheng, Mingzeng Peng, Zhuo Liu, Shuyu Li, Rongcheng Han, Han Ouyang, Yubo Fan, Caofeng Pan, Weiguo Hu, Junyi Zhai, Zhou Li, Zhong Lin Wang
Summary: This study developed a force mapping method based on a light nano-antenna array with the use of piezo-phototronic effect, achieving high-resolution dynamic mapping of cell-generated force. The methodology can be applied for fundamental study of cardiomyocyte behavior and provide valuable information for heart-related research.
Editorial Material
Multidisciplinary Sciences
Jeffrey A. Farrell
Summary: CellOracle, a computational tool, can predict the interaction of gene networks in programming cell identity during embryonic development. This tool will contribute to a better understanding of the regulation of development.
Review
Cell Biology
Jonathan Kuhn, Yiyan Lin, Peter N. Devreotes
Summary: Dictyostelium discoideum has been a valuable tool for dissecting eukaryotic cell biology due to its short growth cycle and genetic tractability. Recent advances in imaging, synthetic biology, and computational analysis allow researchers to tune the activity of individual molecules in living cells and measure the effects on cellular motility and signaling. This enables a better understanding and control of cell migration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Multidisciplinary Sciences
Alain Chedotal
Summary: The GPC3-Unc5 protein complex is found to regulate cell migration in embryos and cancer, indicating a similarity in the migration mechanisms of developing neurons and metastatic cancer cells.
Article
Multidisciplinary Sciences
Zunming Zhang, Rui Tang, Xinyu Chen, Lauren Waller, Alston Kau, Anthony A. Fung, Bien Gutierrez, Cheolhong An, Sung Hwan Cho, Lingyan Shi, Yu-Hwa Lo
Summary: We have developed a high-throughput technique that can relate the positions of individual cells to their three-dimensional imaging features. This technique is particularly useful for nonadherent cells and overcomes the limitations of existing spatial biology methodologies. Our approach involves recording 3D cell images at high throughput using a custom 3D imaging flow cytometer and dispensing cells in a FIFO manner using a robotic cell placement platform. Experiments with human cancer cell lines have demonstrated the feasibility of mapping 3D cell images to their locations on a membrane filter for a large number of cells in a short time. This technology and method bridge the gap between single-cell image analysis and single-cell molecular analysis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Arthur Charles-Orszag, Samuel J. Lord, R. Dyche Mullins
Summary: The study overcame significant technical challenges in extremophile cell biology by using a newly developed imaging system that allowed observation and quantification of cell division dynamics in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius with unprecedented resolution. This system also revealed previously undescribed dynamic cell shape changes, cell motility, and cell-cell interactions, shedding new light on the high-temperature lifestyle of this organism.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Aleksandr Ianevski, Anil K. Giri, Tero Aittokallio
Summary: We developed a computational platform, ScType, for automated and fast cell-type identification using scRNA-seq data and a comprehensive cell marker database. ScType provides unbiased and accurate cell type annotations by ensuring the specificity of marker genes across cell clusters and types. It can also distinguish between healthy and malignant cell populations based on single-nucleotide variant calling.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Multidisciplinary Sciences
Silke Hauf
Summary: A chance observation has uncovered that cells produce multiple variants of a crucial protein essential for cell division, and the proportion of these protein isoforms determines the extent to which division can be postponed in the event of errors.
Review
Cell Biology
Huake Cao, Yuechen Wang, Ning Zhang, Siyuan Xia, Pengfei Tian, Li Lu, Juan Du, Yinan Du
Summary: This article reviews the progress in RNA imaging and mapping RNA-protein interactions, with a focus on the precise CRISPR-Cas13-mediated method. This method has potential for real-time RNA imaging and recognition of RNA-protein interactions, and provides new ideas for optimizing the method.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Jingruo Chen, Shufen Li, Di Ma, Lilan Li, Weihua Zhuang, Mao Chen
Summary: This study developed a LD-specific fluorescence probe TBI, which could precisely target and image lipid droplets in cells and atherosclerotic tissues. TBI exhibited excellent biocompatibility and imaging performance, making it a potential tool for investigating the biological functions and pathological roles of lipid droplets.
Article
Multidisciplinary Sciences
Masato Kojima, Takanori Harada, Takahiro Fukazawa, Sho Kurihara, Isamu Saeki, Shinya Takahashi, Eiso Hiyama
Summary: Single-cell sequencing can accurately represent tumor heterogeneity, with MDA-based WGA method showing higher amplification efficiency and coverage depth. The PCR-based WTA method accurately amplifies mRNA from a single cell, showing specific gene expression patterns.
SCIENTIFIC REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Daniel Castranova, Bakary Samasa, Marina Venero Galanternik, Hyun Min Jung, Van N Pham, Brant M. Weinstein
Summary: Research on zebrafish has revealed the presence of a meningeal lymphatic network similar to that found in mammals, establishing zebrafish as an important new model for experimental analysis of meningeal lymphatic development.
CIRCULATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Fengying Wu, Jue Fan, Yayi He, Anwen Xiong, Jia Yu, Yixin Li, Yan Zhang, Wencheng Zhao, Fei Zhou, Wei Li, Jie Zhang, Xiaosheng Zhang, Meng Qiao, Guanghui Gao, Shanhao Chen, Xiaoxia Chen, Xuefei Li, Likun Hou, Chunyan Wu, Chunxia Su, Shengxiang Ren, Margarete Odenthal, Reinhard Buettner, Nan Fang, Caicun Zhou
Summary: This study utilized single-cell RNA sequencing to analyze 42 late-stage NSCLC patients, revealing significant heterogeneity in tumors in terms of cellular composition, chromosomal structure, developmental trajectory, and the identification of rare cell types. The research also highlighted the correlation between tumor heterogeneity and tumor-associated neutrophils.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Chia-Lang Hsu, Chieh-Fan Yin, Yi-Wen Chang, Ya-Chih Fan, Shih-Han Lin, Yu-Ching Wu, Hsuan-Cheng Huang, Hsueh-Fen Juan
Summary: SNHG1 is an oncogenic long non-coding RNA that is aberrantly expressed in different tumor types. It plays a crucial role in maintaining the identity of neuroblastoma (NB) through chromatin regulation and interacts with core regulatory circuitry transcription factors.
CELL DEATH & DISEASE
(2022)
Article
Chemistry, Multidisciplinary
Federico Dona, Cagakan Ozbalci, Andrea Paquola, Federica Ferrentino, Stephen J. Terry, Elisabeth M. Storck, Gaoge Wang, Ulrike S. Eggert
Summary: The membrane protein stomatin is found to be involved in the cytokinesis step of cell division, even though it is not a lipid biosynthetic enzyme. Depletion of stomatin leads to changes in cellular lipidomes, and exogenous phosphatidylcholines can rescue these defects.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Cell Biology
Rajarshi Chakrabarti, Wei-Ke Ji, Radu V. Stan, Jaime de Juan-Sanz, Timothy A. Ryan, Henry N. Higgs
JOURNAL OF CELL BIOLOGY
(2018)
Article
Cell Biology
Wei-Ke Ji, Rajarshi Chakrabarti, Xintao Fan, Lori Schoenfeld, Stefan Strack, Henry N. Higgs
JOURNAL OF CELL BIOLOGY
(2017)
Article
Neurosciences
Alexa Kiss, Irmgard Fischer, Tatjana Kleele, Thomas Misgeld, Friedrich Propst
FRONTIERS IN CELLULAR NEUROSCIENCE
(2018)
Article
Cell Biology
A. Mu, Tak Shun Fung, Arminja N. Kettenbach, Rajarshi Chakrabarti, Henry N. Higgs
NATURE CELL BIOLOGY
(2019)
Article
Cell Biology
Tak Shun Fung, Wei-Ke Ji, Henry N. Higgs, Rajarshi Chakrabarti
JOURNAL OF CELL SCIENCE
(2019)
Article
Cell Biology
Mengzhe Wang, Tatjana Kleele, Yan Xiao, Gabriela Plucinska, Petros Avramopoulos, Stefan Engelhardt, Markus H. Schwab, Matthias Kneussel, Tim Czopka, Diane L. Sherman, Peter J. Brophy, Thomas Misgeld, Monika S. Brill
Summary: Neuronal remodeling and myelination are two crucial processes during neurodevelopment, their interplay remains largely unknown. Studies show that local axon remodeling delays myelination onset and node formation, while glial differentiation does not impact the outcome of axon remodeling. Delayed myelination is triggered by increased transport of signaling factors that initiate myelination, instead of a limited supply of structural components.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Tamutenda Chidawanyika, Rajarshi Chakrabarti, Kathryn S. Beauchemin, Henry N. Higgs, Surachai Supattapone
Summary: The study found that SEC24A plays an important role in regulating Ca2+ flux between the endoplasmic reticulum and mitochondria, which impacts processes dependent on mitochondrial Ca2+ levels such as autophagy and apoptosis.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Tatjana Kleele, Timo Rey, Julius Winter, Sofia Zaganelli, Dora Mahecic, Helene Perreten Lambert, Francesco Paolo Ruberto, Mohamed Nemir, Timothy Wai, Thierry Pedrazzini, Suliana Manley
Summary: Mitochondrial fission is a highly regulated process that can impact cell function and health. Depending on the location and manner of division, mitochondria can be divided into two types, one for clearing damaged material and undergoing mitophagy, and the other for mitochondrial proliferation.
Article
Cell Biology
Dora Mahecic, Lina Carlini, Tatjana Kleele, Adai Colom, Antoine Goujon, Stefan Matile, Aurelien Roux, Suliana Manley
Summary: This study utilized live-cell structured illumination microscopy to capture the dynamics of constricting mitochondria in mammalian cells. By analyzing the diameters of tubules emerging from mitochondria and implementing a fluorescence lifetime-based mitochondrial membrane tension sensor, it was discovered that mitochondria are indeed under tension during fission. Upon perturbations reducing mitochondrial tension, constrictions initiated at the same rate but were less likely to divide.
Article
Biochemistry & Molecular Biology
Tak Shun Fung, Rajarshi Chakrabarti, Jana Kollasser, Klemens Rottner, Theresia E. B. Stradal, Frieda Kage, Henry N. Higgs
Summary: Mitochondrial damage triggers metabolic changes and mitophagy in cells. This study reveals the mechanism of a rapid peri-mitochondrial actin polymerization called acute damage-induced actin (ADA). The activation of ADA involves multiple signaling pathways and proteins, and it inhibits the rapid mitochondrial shape changes that occur upon mitochondrial depolarization.
Article
Multidisciplinary Sciences
Shivaprasad H. Sathyanarayana, Jasmine A. Saunders, Jacob Slaughter, Kamran Tariq, Rajarshi Chakrabarti, Madhumala K. Sadanandappa, Bryan W. Luikart, Giovanni Bosco
Summary: This study investigated the interaction between PTEN and FMRP in the development of hippocampal granule neurons. The findings suggest that PTEN protein expression is negatively regulated by FMRP, and disruptions in this regulation may contribute to the pathogenesis of Fmr1-related fragile X neurodevelopmental disorders. The study also showed that heterozygosity of Pten can partially restore cellular phenotypes caused by Fmr1 knockout, while complete deletion of both Pten and Fmr1 results in severe abnormalities in neuron development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Rojapriyadharshini Gandhimathi, Dorothea Pinotsi, Mario Koehler, Jorg Mansfeld, Caroline Ashiono, Tatjana Kleele, Sumit Pawar, Ulrike Kutay
Summary: During mitotic entry, nuclear pore complexes (NPCs) disintegrate and so do the large nuclear and cytoplasmic rings formed by Y-complexes. Y-complex Nups dissociate slower than other Nups during in vitro NPC disassembly and remain associated with mitotic ER membranes. Super-resolution microscopy reveals two types of higher-order assemblies of mitotic Y-complexes: large clusters at kinetochores and small focal ER-associated structures, which refine current models of nuclear reassembly.
Review
Biochemistry & Molecular Biology
Rajarshi Chakrabarti, Miriam Lee, Henry N. Higgs
Summary: Actin filaments play multiple roles in the secretory pathway and endosome dynamics in mammals, affecting Golgi structure maintenance, membrane cargo release, endocytosis, and endosomal sorting. The specific mechanisms and functions of actin in these processes require further investigation to fully understand membrane dynamics and related pathways like autophagy.
