Journal
MOLECULAR BIOTECHNOLOGY
Volume 63, Issue 8, Pages 651-675Publisher
SPRINGERNATURE
DOI: 10.1007/s12033-021-00337-4
Keywords
DNA barcode; Bamboo; Taxonomy; Phylogeny; Molecular technology
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Bamboo, with its diverse species, benefits from the utilization of DNA barcoding technology for classification and identification, overcoming challenges of traditional methods. Different barcode regions such as matK, rbcL, and rpoB are used to distinguish bamboo species from different genera.
Bamboo, a gramineous plant belonging to the family Poaceae, comprises of 1575 species from 116 genera across the globe. It has the ability to grow and evolve on degraded land and hence, can be utilized in the various applications as an alternative for plastic and wood. DNA barcoding, a long genomic sequence, identifies barcode region which shows species-specific nucleotide differences. This technology is considered as advanced molecular technique utilized for characterization and classification of the various species by applying distinctive molecular markers. Recent investigations revealed the potential application of various barcode regions such as matK, rbcL, rpoB, rpoC1, psbA-trnH, and ITS2, in identification of many bamboo species from different genus. In this review we comprehensively discussed the relevance of DNA barcoding as a tool in classification/identification of various bamboo species. We highlighted the methodology, how this advance technology overcomes the challenges associated with traditional methods along with prospects for future research.
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