Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 126, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2021.112178
Keywords
Chitosan scaffolds; Microchannel; Cell infiltration; 3D printing microfiber templates
Categories
Funding
- National Natural Science Foundation of China (NSFC) [82072491, 81972063, 31900967]
- Natural Science Foundation of Tianjin [19JCQNJC09300]
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Chitosan scaffolds with adjustable microchannels were successfully fabricated using a combination of 3D printing and freeze-drying methods, allowing precise control of microchannel arrangement, diameter, and density. The scaffolds promoted cell growth and distribution in vitro, and tissue ingrowth and vascular formation in vivo. This study opens up new possibilities for the development of chitosan scaffolds in tissue engineering and regenerative medicine.
Microchannels are effective means of enabling the functional performance of tissue engineering scaffolds. Chitosan, a partial deacetylation derivative of chitin, exhibiting excellent biocompatibility, has been widely used in clinical practice. However, development of chitosan scaffolds with controllable microchannels architecture remains an engineering challenge. Here, we generated chitosan scaffolds with adjustable microchannel by combining a 3D printing microfiber templates-leaching method and a freeze-drying method. We can precisely control the arrangement, diameter and density of microchannel within chitosan scaffolds. Moreover, the integrated bilayer scaffolds with the desired structural parameters in each layer were fabricated and exhibited no delamination. The flow rate and volume of the simulated fluid can be modulated by diverse channels architecture. Additionally, the microchannel structure promoted cell survival, proliferation and distribution in vitro, and improved cell and tissue ingrowth and vascular formation in vivo. This study opens a new road for constructing chitosan scaffolds, and can further extend their application scope across tissue engineering and regenerative medicine.
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