Associations between IVIM histogram parameters and histopathology in rectal cancer

Purpose: Histogram analysis can better reflect tumor heterogeneity than conventional imaging analysis. The present study analyzed possible correlations between histogram analysis parameters derived from Intravoxelincoherent imaging (IVIM) and histopathological features in rectal cancer (RC). Methods: Seventeen patients with histopathologically proven rectal adenocarcinomas were retrospectively acquired. In all cases, pelvic MRI was performed. Diffusion weighted imaging was obtained using a multi-slice single-shot echo-planar imaging sequence with b values of 0, 50, 200, 500 and 1000 s/mm(2). Simplified IVIM analysis was performed using the IntelliSpace portal, version 10 and the following images were generated: f (perfusion fraction) map, D (true diffusion coefficient) map, and ADC map utilizing all b-values. Histogram based analysis of signal intensities was performed for every IVIM map using an in-house matlab tool. Histopathology was investigated using Ki 67 specimens with calculation of Ki 67-index and cellularity. CD31 stained specimens were used for calculation of microvessel density (MVD). Results: There were statistically significant correlations between Ki 67 index and mode derived from ADC as well as entropy from f, r=-0.50, p=.04 and r=-0.55, p=.02, respectively. MVD correlated well with parameters derived from f. Conclusion: IVIM histogram analysis parameters can reflect histopathology in RC. ADC and D values are associated with proliferation potential. Perfusion fraction f is associated with MVD.

Automated summary

Histogram analysis from IVIM imaging can provide insights into tumor heterogeneity in rectal cancer. There are significant correlations between Ki 67 index and parameters from ADC and f maps, as well as between MVD and parameters derived from the f map. IVIM analysis parameters can reflect histopathological features in rectal cancer, with ADC and D values associated with proliferation potential and perfusion fraction f associated with microvessel density.