Article
Chemistry, Analytical
Madisson Chabas, Olivier Pible, Jean Armengaud, Beatrice Alpha-Bazin
Summary: To accurately and rapidly identify microorganisms from complex microbiota for clinical diagnostic purposes, an innovative label-free multiplexing method for the identification of microorganisms using tandem mass spectrometry is proposed in this paper. The method involves offline reversed-phase fractionation of individual peptidomes and achieves multiplexing by mixing fractions of staged hydrophobicity. This approach enables the identification of up to 21 microorganisms in a single 60-minute run without the use of labeling reagents, providing new perspectives for high-throughput proteotyping of bacteria in large culturomics projects.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Annunziata Lapolla, Pietro Traldi
Summary: Gestational diabetes mellitus (GDM) leads to complications for both mothers and infants, and is associated with changes in placenta structure and function. Various mass spectrometric approaches can be used to study the protein profile of placenta. These techniques have shown differences in protein expression between healthy and GDM placenta samples.
Article
Chemistry, Analytical
Yuming Jiang, Alexandre Hutton, Caleb W. Cranney, Jesse G. Meyer
Summary: This article introduces an improved direct infusion and ion mobility method for proteome analysis, and proposes a label-free quantification method DISPA-LFQ, which can rapidly identify and quantify proteins in complex mixtures. This technology has important applications in drug screening, biomarker discovery, and clinical analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Khushman Taunk, Debasish Paul, Raju Dabhi, Chanukuppa Venkatesh, Saikiran Jajula, Venkateshwarlu Naik, Anup Tamhankar, Tufan Naiya, Manas Kumar Santra, Srikanth Rapole
Summary: Proteins play a crucial role in modern biology, and the study of proteomics is important for understanding the protein content in biological samples. This study developed a simple single step method for efficient protein extraction, applicable to a wide range of biological samples.
Review
Spectroscopy
Marco Roverso, Raghav Dogra, Silvia Visentin, Silvia Pettenuzzo, Luca Cappellin, Paolo Pastore, Sara Bogialli
Summary: This article focuses on the different analytical methods and mass spectrometry-based instrumental platforms used in the study of biomarkers for gestational diabetes mellitus (GDM). Current studies only report preliminary findings, and further efforts are required to determine definitive GDM biomarkers.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Medical Laboratory Technology
Yuqi Yang, Zhaoping Pan, Fang Guo, Huihui Wang, Wei Long, Huiyan Wang, Bin Yu
Summary: This study identified differentially expressed metabolites in placenta of GDM patients and their correlation with clinical parameters of mothers and newborns. Lipids and lipid-like molecules, especially unsaturated fatty acids, were found to be the main differential metabolites. Lipid metabolites such as linoleic acid and alpha-linolenic acid appear to be potential biomarkers for predicting and diagnosing GDM.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Review
Chemistry, Medicinal
Jichao Sun, Nayana Prabhu, Jun Tang, Fan Yang, Lin Jia, Jinan Guo, Kefeng Xiao, Wai Leong Tam, Par Nordlund, Lingyun Dai
Summary: Assessing drug-target engagement on a proteome-wide scale is crucial for understanding molecular mechanisms and developing more effective drugs. Label-free target deconvolution approaches have gained attention as they avoid chemical modification and offer insights into drug action mechanisms.
MEDICINAL RESEARCH REVIEWS
(2021)
Article
Immunology
Jiangbei Yuan, Zhangfu Li, Zewei Lin, Siyu Yao, Yuewen Han, Qihuan Fu, Jikui Liu
Summary: The Steap3-Gm2a axis restricts the phagosomal escape of L. monocytogenes and may serve as a potential molecular drug target for antibacterial treatment.
MICROBES AND INFECTION
(2022)
Article
Agriculture, Dairy & Animal Science
Andrzej Gawor, Anna Ruszczynska, Anna Konopka, Grzegorz Wryk, Marian Czauderna, Ewa Bulska
Summary: This study investigated the effects of different dietary supplements, including selenium, on protein expression in animal tissues using global differential proteomic experiments and protein-protein interaction network analysis. Selenium was found to play a significant role in regulating important biological functions such as cell adhesion and heart development.
Article
Biochemistry & Molecular Biology
Diego Julian Vesga-Jimenez, Cynthia A. Martin-Jimenez, Adriana Grismaldo Rodriguez, Andres Felipe Aristizabal-Pachon, Andres Pinzon, George E. Barreto, David Ramirez, Janneth Gonzalez
Summary: This study used a proteomic approach to investigate the protective effects of tibolone on normal human astrocytes exposed to supraphysiological levels of palmitic acid. The results showed that tibolone restored the changes in processes such as translation, transport, autophagy, and apoptosis caused by palmitic acid and increased the expression of proteins related to cell survival. Interestingly, the proteins ARF3 and IPO7 were identified as important proteins related to transport and translation processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Fredrick J. Rosario, Sammy Pardo, Trond M. Michelsen, Kathryn Erickson, Lorna Moore, Theresa L. Powell, Susan T. Weintraub, Thomas Jansson
Summary: By studying the secreted proteins of PHT cells isolated from normal term placental villus tissue, a total of 1,344 previously unknown proteins were identified, involving processes such as energy metabolism, carbon metabolism, and glycolysis. Some proteins have slow synthesis rates, and there is significant overlap with proteins actually secreted by the placenta.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Anna Papadopoulou, Eirini Thymara, Eirini Maratou, George Kanellopoulos, Vasiliki Papaevangelou, Sophia Kalantaridou, Spyridon Kanellakis, Pinelopi Triantafyllidou, George Valsamakis, George Mastorakos
Summary: This study evaluated the expression of placental LRP5 and sclerostin in pregnancies with gestational diabetes mellitus (GDM) and investigated their associations with umbilical sclerostin concentrations and clinical outcomes. The results showed that placental sclerostin and LRP5 were significantly higher in GDM mothers compared with non-GDM mothers, and placental sclerostin expression was positively correlated with maternal pregestational body mass index and fasting glucose concentrations.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Reham Atallah, Juergen Gindlhuber, Wolfgang Platzer, Thomas Baernthaler, Eva Tatzl, Wolfgang Toller, Jasmin Strutz, Sonja Rittchen, Petra Luschnig, Ruth Birner-Gruenberger, Christian Wadsack, Akos Heinemann
Summary: Placental hypervascularization has been associated with gestational diabetes mellitus (GDM), and the succinate-SUCNR1 axis may play a role in this process. Succinate levels were higher in placental tissue from women with GDM, along with upregulated SUCNR1 expression. In vitro experiments showed that succinate promoted angiogenesis in endothelial cells, while knockdown of SUCNR1 suppressed VEGF expression and migration in response to succinate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Elena G. Bernea, Viorel Suica, Elena Uyy, Aurel Cerveanu-Hogas, Raluca M. Boteanu, Luminita Ivan, Iuliana Ceausu, Doina A. Mihai, Constantin Ionescu-Tirgoviste, Felicia Antohe
Summary: This study found that the protein composition of serum exosomes in gestational diabetes mellitus (GDM) patients is associated with inflammation and vascular dysfunction. Pathways related to complement and coagulation cascades, platelet activation, prothrombotic factors, and cholesterol metabolism may play important roles in the pathophysiology of GDM.
Article
Biochemistry & Molecular Biology
Valeria C. Nogueira, Valzimeire do N. de Oliveira, Maria I. F. Guedes, Bradley J. Smith, Jose E. da C. Freire, Nidyedja G. G. Gonsalves, Ana C. de O. M. Moreira, Renato de A. Moreira
Summary: This study identified differentially expressed proteins in the serum of T1D patients using LC-MS/MS analysis, and found that these proteins are associated with the immune system, inflammation, and growth hormone stimulus response. The results suggest that these proteins could be potential targets for future investigations and validation of protein biomarkers in T1D.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Spectroscopy
Beatrice Zoanni, Maura Brioschi, Alice Mallia, Erica Gianazza, Sonia Eligini, Marina Carini, Giancarlo Aldini, Cristina Banfi
Summary: This review summarizes the important roles of human serum albumin (HSA) in cardiovascular diseases (CVD) and heart failure, emphasizing the physiological functions of highly abundant plasma proteins should not be neglected.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Spectroscopy
Erica Gianazza, Beatrice Zoanni, Alice Mallia, Maura Brioschi, Gualtiero Colombo, Cristina Banfi
Summary: This narrative review highlights the potential of lipoprotein-associated proteins in cardiovascular risk assessment and emphasizes the importance of high-throughput proteomic analysis.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Sonia Eligini, Erica Gianazza, Alice Mallia, Stefania Ghilardi, Cristina Banfi
Summary: Macrophages are versatile cells that can adapt their characteristics and functions to different environments. They play important roles in maintaining homeostasis and are also involved in various human diseases, including atherosclerosis. Proteomics has been widely used to study macrophages, and analyzing the proteome of different macrophage phenotypes can provide insights into their roles in atherosclerosis and identify potential therapeutic targets. In this review, we discuss different proteomic techniques used to profile macrophage phenotypes and summarize findings that contribute to understanding macrophage functions and developing strategies to combat atherosclerosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Marcos Leal Brioschi, Carlos Dalmaso Neto, Marcos de Toledo, Eduardo Borba Neves, Jose Viriato Coelho Vargas, Manoel Jacobsen Teixeira
Summary: This study proposed an infrared image-based method for febrile and subfebrile people screening to comply with the society need for alternative, quick response, and effective methods for COVID-19 contagious people screening.
JOURNAL OF THERMAL BIOLOGY
(2023)
Article
Imaging Science & Photographic Technology
Flavio Leme Ferrari, Marcos Leal Brioschi, Carlos Dalmaso Neto, Carlos Roberto de Medeiros
Summary: The authors developed a new non-invasive dermatological infrared macroimaging analysis technique (MacroIR) that evaluates microvascular, inflammatory, and metabolic changes in skin and mucosal lesions. The technique combines naked eye observation, polarized light dermatoscopy (PLD), and MacroIR to compare results. Ten cases were evaluated using a smartphone coupled with a dermatoscope and a macro lens integrated far-infrared transducer into specific software. MacroIR imaging showed promise in improving dermatological diseases diagnosis.
JOURNAL OF IMAGING
(2023)
Review
Biochemistry & Molecular Biology
Erica Gianazza, Maura Brioschi, Ada Iezzi, Giuseppe Paglia, Cristina Banfi
Summary: Lipid-lowering therapies are widely used to prevent ASCVD and related mortality. Omics technologies have been employed to study the mechanisms and effects of these drugs, aiming to improve treatment efficacy and safety. Pharmacometabolomics focuses on studying the effects of drugs on metabolic pathways and the variation of treatment response. This review summarizes significant metabolomic studies on lipid-lowering therapies, including statins, fibrates, and novel drugs and nutraceutical approaches. Integrating pharmacometabolomics data with other omics approaches can help understand the biological mechanisms of lipid-lowering drugs and improve treatment efficacy while reducing side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Cristina Banfi, Alice Mallia, Stefania Ghilardi, Maura Brioschi, Erica Gianazza, Sonia Eligini, Pelin Sahlen, Roberta Baetta
Summary: PCYOX1 is a regulator of adipogenesis, acting through multiple key factors. Its absence reduces adipogenesis and inflammatory markers, making it a potential adipogenic factor with therapeutic potential.
Article
Biochemistry & Molecular Biology
Marta Zara, Andrea Baggiano, Patrizia Amadio, Jeness Campodonico, Sebastiano Gili, Andrea Annoni, Gianluca De Dona, Maria Ludovica Carerj, Francesco Cilia, Alberto Formenti, Laura Fusini, Cristina Banfi, Paola Gripari, Calogero Claudio Tedesco, Maria Elisabetta Mancini, Mattia Chiesa, Riccardo Maragna, Francesca Marchetti, Marco Penso, Luigi Tassetti, Alessandra Volpe, Alice Bonomi, Giancarlo Marenzi, Gianluca Pontone, Silvia Stella Barbieri
Summary: This study found that the expression of CD41-CD61 in sEVs can reflect the CMR-assessed ischemic damage after STEMI, providing a new strategy for the timely identification of high-risk patients and their treatment optimization.
Article
Chemistry, Multidisciplinary
Raghav Dogra, Marco Roverso, Giuseppe Di Bernardo, Alessandra Zanut, Fazel A. Monikh, Silvia Pettenuzzo, Paolo Pastore, Sara Bogialli
Summary: Glyphosate (GLY), a widely used herbicide, has raised concerns due to its toxicity and mobility in water. The simultaneous spread of similar herbicides, such as glufosinate (GLUF) and aminomethylphosphonic acid (AMPA), has also caused environmental concerns. Magnetic nanoparticles (MNPs) coated with polydopamine and functionalized with metal ions (Ti(iv), Zr(iv), and Cu(ii)) showed promise in selectively removing GLY, AMPA, and GLUF from water, and were effective even in the presence of competitive species. MNPs functionalized with Ti(iv) and Zr(iv) performed more efficiently than Cu(ii)-functionalized MNPs.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Inorganic & Nuclear
Mattia Benedet, Davide Barreca, Ettore Fois, Roberta Seraglia, Gloria Tabacchi, Marco Roverso, Gioele Pagot, Cristiano Invernizzi, Alberto Gasparotto, Alexandra A. Heidecker, Alexander Poehig, Emanuela Callone, Sandra Dire, Sara Bogialli, Vito Di Noto, Chiara Maccato
Summary: The coordination sphere engineering of three heteroleptic Ni(ii) beta-diketonate-diamine adducts was studied, and it was found that the chemico-physical characteristics of the compounds directly depend on the functional groups in the diketonate ligands. Preliminary thermal CVD experiments demonstrated the suitability of the precursors for the obtainment of NiO films with flat and homogeneous surfaces.
DALTON TRANSACTIONS
(2023)
Article
Medicine, General & Internal
Marcelo Pastor Almada Davalos, Marcos Leal Brioschi, Samir Ezequiel da Rosa, Gabriel Carneiro Brioschi, Eduardo Borba Neves
Summary: This study explored the potential of dual infrared-visual thermography (IRVT) to identify perforator veins (PV) in asymptomatic veins. The results showed that IRVT effectively distinguishes varying degrees of vascular reflux severity. IRVT shows promise as a non-invasive, radiation-free tool to enhance PV identification.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Guglielmina Froldi, Francesco Benetti, Andrea Mondin, Marco Roverso, Elisa Pangrazzi, Francine Medjiofack Djeujo, Paolo Pastore
Summary: The study of medicinal plants and their active compounds is essential for maintaining traditional medicine knowledge and developing natural drugs with lower environmental impact. This study focused on the seeds of Pterodon emarginatus from Brazil, and different preparations were obtained and characterized chemically. The study also evaluated their activity and potential as drug candidates.
Article
Medicine, General & Internal
Bruno Veloso Fracasso, Renato Bender Castro, Marcos Leal Brioschi, Tais Malysz
Summary: This study used infrared point-of-care thermography to analyze the facial temperature distribution of 24 female participants with chronic migraine. The findings revealed significant temperature asymmetry in women with right-sided unilateral headaches, particularly in the right frontal and temporal regions. However, caution is advised in interpreting these results, as further research with larger samples is needed.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Luca Morgan, Giulio Pavan, Nicola Demitri, Chiara Alberoni, Thomas Scattolin, Marco Roverso, Sara Bogialli, Alessandro Aliprandi
Summary: A unified metal-free procedure was used to synthesize and characterize a selection of Thermally Activated Delayed Fluorescence (TADF) emitters. A promising compound with terephthalonitrile as the acceptor and diphenylamines as donors showed significantly higher electrochemiluminescence efficiency than the standard [Ru(bpy)3](PF6)2. A novel water-soluble TADF emitter (Na4[4DPASO3TPN]) was also synthesized and characterized for electrochemiluminescence in an aqueous medium.