Cell-Free Biosynthesis to Evaluate Lasso Peptide Formation and Enzyme–Substrate Tolerance

Heterologous Production of Lasso Peptide Capistruin in a Burkholderia Host

(2020) Sylvia Kunakom et al.   ACS Synthetic Biology

Identification, Cloning and Heterologous Expression of the Gene Cluster Directing RES-701-3, -4 Lasso Peptides Biosynthesis from a Marine Streptomyces Strain

(2020) Daniel Oves-Costales et al.   Marine Drugs

Minimal lactazole scaffold for in vitro thiopeptide bioengineering

(2020) Alexander A. Vinogradov et al.   Nature Communications

How to harness biosynthetic gene clusters of lasso peptides

(2020) Shinya Kodani et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

A Cell‐Free Platform Based on Nisin Biosynthesis for Discovering Novel Lanthipeptides and Guiding their Overproduction In Vivo

(2020) Ran Liu et al.   Advanced Science

Class IV Lasso Peptides Synergistically Induce Proliferation of Cancer Cells and Sensitize Them to Doxorubicin

(2020) Jaime Felipe Guerrero-Garzón et al.   iScience

Flexizyme-Enabled Benchtop Biosynthesis of Thiopeptides

(2019) Steven R. Fleming et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin

(2019) Nathaniel R. Braffman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural signatures of the class III lasso peptide BI-32169 and the branched-cyclic topoisomers using trapped ion mobility spectrometry–mass spectrometry and tandem mass spectrometry

(2019) Kevin Jeanne Dit Fouque et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

The Lasso Peptide Siamycin-I Targets Lipid II at the Gram-Positive Cell Surface

(2019) Stephanie Tan et al.   ACS Chemical Biology

Factors Governing the Thermal Stability of Lasso Peptides

(2019) Julian D. Hegemann   CHEMBIOCHEM

Cell-free gene expression: an expanded repertoire of applications

(2019) Adam D. Silverman et al.   NATURE REVIEWS GENETICS

Structure of ribosome-bound azole-modified peptide phazolicin rationalizes its species-specific mode of bacterial translation inhibition

(2019) Dmitrii Y. Travin et al.   Nature Communications

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

(2019) Weston Kightlinger et al.   Nature Communications

Genome Mining and Heterologous Expression Reveal Two Distinct Families of Lasso Peptides Highly Conserved in Endofungal Bacteria

(2019) Evgeni V. Bratovanov et al.   ACS Chemical Biology

Albusnodin: an acetylated lasso peptide from Streptomyces albus

(2018) Chuhan Zong et al.   CHEMICAL COMMUNICATIONS

An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts

(2018) Jimmy Mevaere et al.   Scientific Reports

Investigation of the Biosynthesis of the Lasso Peptide Chaxapeptin Using an E. coli-Based Production System

(2018) Helena Martin-Gómez et al.   JOURNAL OF NATURAL PRODUCTS

Enzymatic Reconstitution and Biosynthetic Investigation of the Lasso Peptide Fusilassin

(2018) Adam J. DiCaprio et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Heterologous and in Vitro Reconstitution of Fuscanodin, a Lasso Peptide from Thermobifida fusca

(2018) Joseph D. Koos et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A new genome-mining tool redefines the lasso peptide biosynthetic landscape

(2017) Jonathan I Tietz et al.   Nature Chemical Biology

Dissection of goadsporin biosynthesis by in vitro reconstitution leading to designer analogues expressed in vivo

(2017) Taro Ozaki et al.   Nature Communications

Chimeric Leader Peptides for the Generation of Non-Natural Hybrid RiPP Products

(2017) Brandon J. Burkhart et al.   ACS Central Science

ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules

(2016) Haim Ashkenazy et al.   NUCLEIC ACIDS RESEARCH

Cell-Free Synthetic Biology: Engineering Beyond the Cell

(2016) Jessica G. Perez et al.   Cold Spring Harbor Perspectives in Biology

In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis

(2016) Anthony W. Goering et al.   ACS Synthetic Biology

Lasso Peptides: An Intriguing Class of Bacterial Natural Products

(2015) Julian D. Hegemann et al.   ACCOUNTS OF CHEMICAL RESEARCH

Construction of Lasso Peptide Fusion Proteins

(2015) Chuhan Zong et al.   ACS Chemical Biology

Characterization of Sviceucin from Streptomyces Provides Insight into Enzyme Exchangeability and Disulfide Bond Formation in Lasso Peptides

(2015) Yanyan Li et al.   ACS Chemical Biology

Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

(2015) Frank J. Piscotta et al.   CHEMICAL COMMUNICATIONS

Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol

(2015) Jennifer E. Kay et al.   METABOLIC ENGINEERING

A prevalent peptide-binding domain guides ribosomal natural product biosynthesis

(2015) Brandon J Burkhart et al.   Nature Chemical Biology

Biomathematical Description of Synthetic Peptide Libraries

(2015) Timo Sieber et al.   PLoS One

Site-Directed and Global Incorporation of Orthogonal and Isostructural Noncanonical Amino Acids into the Ribosomal Lasso Peptide Capistruin

(2014) Rashed S. Al Toma et al.   CHEMBIOCHEM

Lassomycin, a Ribosomally Synthesized Cyclic Peptide, Kills Mycobacterium tuberculosis by Targeting the ATP-Dependent Protease ClpC1P1P2

(2014) Ekaterina Gavrish et al.   CHEMISTRY & BIOLOGY

Rational Improvement of the Affinity and Selectivity of Integrin Binding of Grafted Lasso Peptides

(2014) Julian D. Hegemann et al.   JOURNAL OF MEDICINAL CHEMISTRY

Amicoumacin A Inhibits Translation by Stabilizing mRNA Interaction with the Ribosome

(2014) Yury S. Polikanov et al.   MOLECULAR CELL

Lasso peptides from proteobacteria: Genome mining employing heterologous expression and mass spectrometry

(2013) Julian D. Hegemann et al.   BIOPOLYMERS

Sungsanpin, a Lasso Peptide from a Deep-Sea Streptomycete

(2013) Soohyun Um et al.   JOURNAL OF NATURAL PRODUCTS

Discovery and Characterization of an Isopeptidase That Linearizes Lasso Peptides

(2013) Mikhail O. Maksimov et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System

(2013) Zachary Z. Sun et al.   ACS Synthetic Biology

Sequence Determinants Governing the Topology and Biological Activity of a Lasso Peptide, Microcin J25

(2012) Rémi Ducasse et al.   CHEMBIOCHEM

Dissecting the Maturation Steps of the Lasso Peptide Microcin J25 in vitro

(2012) Kok-Phen Yan et al.   CHEMBIOCHEM

Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature

(2012) Paul G. Arnison et al.   NATURAL PRODUCT REPORTS

Sequence Diversity in the Lasso Peptide Framework: Discovery of Functional Microcin J25 Variants with Multiple Amino Acid Substitutions

(2011) Si Jia Pan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Insights into the Biosynthesis and Stability of the Lasso Peptide Capistruin

(2009) Thomas A. Knappe et al.   CHEMISTRY & BIOLOGY

Systematic Structure-Activity Analysis of Microcin J25

(2008) Olga Pavlova et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Isolation and Structural Characterization of Capistruin, a Lasso Peptide Predicted from the Genome Sequence of Burkholderia thailandensis E264

(2008) Thomas A. Knappe et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

NCBI BLAST: a better web interface

(2008) M. Johnson et al.   NUCLEIC ACIDS RESEARCH