Proteomic and transcriptomic analyses of saliva and salivary glands from the Asian citrus psyllid, Diaphorina citri

Salivary secretions play critical roles in interactions among insects, insect-vectored pathogens, and host plants. The Asian citrus psyllid Diaphorina citri is a sap-sucking Hemipteran that serves as a vector for Candidatus Liberibacter asiaticus, the causal agent of citrus greening disease (Huanglongbing or HLB). D. citri continuously injects saliva into host plants using specialized stylets so as to feed and transmit the HLB pathogen. Knowledge on the composition and function of salivary proteins of this pest is very limited. In this study, proteomic and transcriptomic approaches were adopted to characterize the protein composition of the saliva and salivary glands in D. citri. A total of 246 and 483 proteins were identified in saliva and dissected salivary glands, respectively, via LC-MS/MS analyses. Comparative analyses of the identified proteins were performed between D. citri and other reported Hemipteran insect species. Transcription levels of the genes coding for the identified proteins were determined via RNA-sequencing among different tissues including salivary glands and other digestive tissues. Identification of putative effectors that are expressed exclusively or abundantly in salivary glands provides the foundation for future functional studies towards the understanding of their roles in interactions among D. citri, HLB pathogen, and their citrus host. Biological significance: This is a systematic analysis on proteins in saliva and dissected salivary glands. A high percentage of novel proteins have been identified due to the large amounts of samples collected. This report gives a more comprehensive repertoire of potential effector proteins that may be possibly involved in modulating host defense, altering nutrient metabolism, and facilitating Ca. L. asiaticus transmission.

Automated summary

This study characterized the protein composition of saliva and dissected salivary glands in the Asian citrus psyllid through proteomic and transcriptomic approaches, providing insights into the roles of salivary proteins in interactions among the psyllid, the HLB pathogen, and citrus host. The identification of putative effectors expressed in salivary glands offers potential targets for future functional studies to understand mechanisms of host-pathogen interactions.