Article
Fisheries
Joseph P. Nowlan, John S. Lumsden, Spencer Russell
Summary: Quantitative PCR assays were developed for detecting Tenacibaculum species, showing low detection limits and suitable amplification efficiencies, but further investigation into other genes is needed. These assays may help elucidate the pathogenesis of tenacibaculosis and aid management practices in the aquaculture industry.
JOURNAL OF FISH DISEASES
(2021)
Article
Microbiology
Quentin Jehanne, Lucie Benejat, Astrid Ducournau, Emilie Bessede, Philippe Lehoursa
Summary: Aliarcobacter butzleri is an emerging gastrointestinal pathogen. This study analyzed the genomes of 30 resistant A. butzleri isolates to propose specific epidemiological cut-off values for certain antibiotics. The findings contribute to the development of a consensus for antimicrobial susceptibility testing for this pathogen.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Joseph P. Nowlan, Scott R. Britney, John S. Lumsden, Spencer Russell
Summary: There is limited understanding of the pathogenesis of tenacibaculosis in Atlantic salmon and few reproducible exposure models for comparison. Different species of Tenacibaculum were used to expose Atlantic salmon, showing varied mortality rates and clinical signs. This experimental infection model will be helpful for further research on the pathogenesis, treatment, and immunity of tenacibaculosis.
Article
Microbiology
Joseph P. Nowlan, Scott R. Britney, John S. Lumsden, Spencer Russell
Summary: Tenacibaculum species, including T. maritimum and T. dicentrarchi, were frequently detected in commercial Atlantic salmon netpen sites, showing a ubiquitous distribution. T. dicentrarchi was found before outbreaks and T. maritimum was rarely identified. Increases in bacterial load were recorded during outbreaks, with more bacteria in summer months, suggesting a seasonal influence on tenacibaculosis. Fish mortalities were related to antimicrobial use and water quality parameters, indicating the need for further research to understand the dynamics of tenacibaculosis outbreaks.
Article
Fisheries
Monica Saldarriaga-Cordoba, Rute Irgang, Ruben Avendano-Herrera
Summary: Tenacibaculum dicentrarchi is an emerging pathogen that causes high economic losses in the salmon industry in Chile. Comparative analysis of the genome sequences of different strains provides insights into the virulence process of this bacterium.
JOURNAL OF FISH DISEASES
(2021)
Article
Rheumatology
Oh Chan Kwon, Min-Chan Park
Summary: This study determined the cut-off values of BASDAI for determining disease activity states in ankylosing spondylitis patients. The study found that BASDAI values of 1.9, 3.5, and 4.9 corresponded to ASDAS-CRP values of 1.3, 2.1, and 3.5, respectively. The agreement between disease activity states based on BASDAI and ASDAS-CRP cut-off values was good.
Article
Fisheries
Rute Irgang, Ruben Avendano-Herrera
Summary: This study analyzes the susceptibility patterns of Tenacibaculum dicentrarchi to tiamulin and suggests that tiamulin is a viable alternative to florfenicol for the treatment of tenacibaculosis in the Chilean salmon industry.
JOURNAL OF FISH DISEASES
(2022)
Article
Microbiology
Yaoyao Lin, Jicheng Yang, Zhenbing Wu, Qianqian Zhang, Shuyi Wang, Jingwen Hao, Lijian Ouyang, Aihua Li
Summary: The abuse of antibiotics in aquaculture has led to an increasing threat to environmental and human health due to the rising antibiotic resistance of aquatic bacteria. This study determined the minimum inhibitory concentration (MIC) of eight antimicrobial agents against Aeromonas strains, and established tentative cut-off values (COWT) for this bacteria species. The obtained COWT values can contribute to the establishment of international COWT for Aeromonas.
Article
Infectious Diseases
O. Karatuna, D. A. B. Dance, E. Matuschek, J. Ahman, P. Turner, J. Hopkins, P. Amornchai, V Wuthiekanun, T-P Cusack, R. Baird, J. Hennessy, R. Norton, M. Armstrong, S. Zange, L. Zoeller, T. Wahab, D. Jacob, R. Grunow, G. Kahlmeter
Summary: This study established MIC and zone diameter distributions for Burkholderia pseudomallei using standard EUCAST methodology, and determined ECOFF values for different antimicrobials. The results showed that the use of standard BMD and disc diffusion methodology for AST of B. pseudomallei has been validated.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Infectious Diseases
Qiwen Yang, Xue Li, Peiyao Jia, Christian Giske, Gunnar Kahlmeter, John Turnidge, Yunsong Yu, Yuan Lv, Minggui Wang, Ziyong Sun, Jie Lin, Yun Li, Bo Zheng, Fupin Hu, Yan Guo, Zhongju Chen, Haixia Li, Ge Zhang, Jingjia Zhang, Wei Kang, Simeng Duan, Tong Wang, Ran Jing, Yingchun Xu
Summary: Epidemiological cut-off values (ECOFFs) of norvancomycin for four Staphylococcus species were determined through a study involving 1199 clinical isolates. The correlation between MICs and zone diameters of norvancomycin was found to be weak in the Staphylococcus species. Additionally, no correlation was observed between ECOFFs of norvancomycin and the mecA gene carriage in Staphylococcus species.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Soo Ji Woo, Myoung Sug Kim, Mi Young Do, Na Young Kim, Min Soo Joo
Summary: This study aimed to determine the antimicrobial susceptibility of aquatic Strep. parauberis strains and establish laboratory-specific epidemiological cut-off (COWT) values to distinguish wild-type (WT) and nonwild-type (NWT) strains. Using 220 Strep. parauberis isolates obtained from diseased fish over 6 years in Korea, COWT values for eight common antimicrobial agents were established. Nine NWT isolates with decreased susceptibility to at least two antimicrobials were identified using COWT values based on NRI.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Infectious Diseases
Victor Mercier, Valerie Letscher-Bru, Marie-Elisabeth Bougnoux, Laurence Delhaes, Francoise Botterel, Daniele Maubon, Frederic Dalle, Alexandre Alanio, Sandrine Houze, Eric Dannaoui, Carole Cassagne, Sophie Cassaing, Marie-Fleur Durieux, Arnaud Fekkar, Jean-Philippe Bouchara, Jean-Pierre Gangneux, Julie Bonhomme, Damien Dupont, Damien Costa, Boualem Sendid, Taieb Chouaki, Nathalie Bourgeois, Antoine Huguenin, Sophie Brun, Caroline Mahinc, Lilia Hasseine, Solene Le Gal, Anne-Pauline Bellanger, Eric Bailly, Florent Morio, Celine Nourrisson, Nicole Desbois-Nogard, Estelle Perraud-Cateau, Anne Debourgogne, Helene Yera, Laurence Lachaud, Milene Sasso
Summary: The study aimed to determine the epidemiological cut-off values (ECVs) of ten antifungal agents in a wide range of yeasts and Aspergillus spp. using gradient concentration strips. Retrospective collection of minimum inhibitory concentrations for various antifungal agents was done from 35 French microbiology laboratories between 2002 and 2020. The calculated ECVs can aid in interpreting minimum inhibitory concentrations and identifying non-wild type isolates.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Respiratory System
Philip W. Fowler, Ivan Barilar, Simone Battaglia, Emanuele Borroni, Angela Pires Brandao, Alice Brankin, Andrea Maurizio Cabibbe, Joshua Carter, Daniela Maria Cirillo, Pauline Claxton, David A. Clifton, Ted Cohen, Jorge Coronel, Derrick W. Crook, Viola Dreyer, Sarah G. Earle, Vincent Escuyer, Lucilaine Ferrazoli, George Fu Gao, Jennifer Gardy, Saheer Gharbia, Kelen Teixeira Ghisi, Arash Ghodousi, Ana Luiza Gibertoni Cruz, Louis Grandjean, Clara Grazian, Ramona Groenheit, Jennifer L. Guthrie, Wencong He, Harald Hoffmann, Sarah J. Hoosdally, Martin Hunt, Zamin Iqbal, Nazir Ahmed Ismail, Lisa Jarrett, Lavania Joseph, Ruwen Jou, Priti Kambli, Rukhsar Khot, Jeff Knaggs, Anastasia Koch, Donna Kohlerschmidt, Samaneh Kouchaki, Alexander S. Lachapelle, Ajit Lalvani, Simon Grandjean Lapierre, Ian F. Laurenson, Brice Letcher, Wan-Hsuan Lin, Chunfa Liu, Dongxin Liu, Kerri M. Malone, Ayan Mandal, Mikael Mansjo, Daniela Matias, Graeme Meintjes, Flavia de Freitas Mendes, Matthias Merker, Marina Mihalic, James Millard, Paolo Miotto, Nerges Mistry, David Moore, Kimberlee A. Musser, Dumisani Ngcamu, Hoang Ngoc Nhung, Stefan Niemann, Kayzad Soli Nilgiriwala, Camus Nimmo, Nana Okozi, Rosangela Siqueira Oliveira, Shaheed Vally Omar, Nicholas Paton, Timothy E. A. Peto, Juliana Maira Watanabe Pinhata, Sara Plesnik, Zully M. Puyen, Marie Sylvianne Rabodoarivelo, Niaina Rakotosamimanana, Paola M. Rancoita, Priti Rathod, Esther Robinson, Gillian Rodger, Camilla Rodrigues, Timothy C. Rodwell, Aysha Roohi, David Santos-Lazaro, Sanchi Shah, Thomas Andreas Kohl, Grace Smith, Walter Solano, Andrea Spitaleri, Philip Supply, Utkarsha Surve, Sabira Tahseen, Nguyen Thuy Thuong, Guy Thwaites, Katharina Todt, Alberto Trovato, Christian Utpatel, Annelies Van Rie, Srinivasan Vijay, Timothy M. Walker, A. Sarah Walker, Robin Warren, Jim Werngren, Maria Wijkander, Robert J. Wilkinson, Daniel J. Wilson, Penelope Wintringer, Yu-Xin Xiao, Yang, Zhao Yanlin, Shen-Yuan Yao, Baoli Zhu
Summary: This study determines the epidemiological cut-off values (ECOFF/ECVs) for 13 anti-tuberculosis compounds, which will facilitate the measurement of drug susceptibility in Mycobacterium tuberculosis. These findings can contribute to personalized tuberculosis treatment.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Dermatology
Bertrand Nyuykonge, Emmanuel E. Siddig, Najwa Adam Mhmoud, Borna A. Nyaoke, Eduard E. Zijlstra, Annelies Verbon, Sahar Bakhiet, Ahmed H. Fahal, Wendy W. J. van de Sande
Summary: The study determined tentative epidemiological cut-off values (ECVs) for itraconazole and ravuconazole in Madurella mycetomatis. The ECVs were found to be 1 mg/L for itraconazole and 0.064 mg/L for ravuconazole. Two CYP51A variants were identified in the wild-type population of M. mycetomatis, with similar MIC distributions for itraconazole and ravuconazole between the two variants. No mutations associated with decreased susceptibility were found.
Article
Infectious Diseases
Yan Guo, Renru Han, Ge Zhang, Qiwen Yang, Feng Xue, Yun Li, Demei Zhu, Fupin Hu
Summary: The epidemiological cut-off values (TECOFFs) of contezolid for various Gram-positive bacteria such as Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, Streptococcus pneumoniae, and Streptococcus agalactiae were determined based on MIC and zone diameter distributions.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Fisheries
Maritza Grandon, Rute Irgang, Jose Saavedra, Marcos Mancilla, Ruben Avendano-Herrera
Summary: Surveillance of antibiotic resistance in fish pathogens is crucial, particularly for Renibacterium salmoninarum in salmon farming. A study in southern Chile revealed an evolving subpopulation of non-wild-type isolates for erythromycin, while isolates were most susceptible to florfenicol and oxytetracycline. This information can be useful for antibiotic surveillance programs and future revisions in MIC values determination methods.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Hector A. Levipan, Ruben Avendano-Herrera
Summary: This study investigated the growth and cytotoxic effect of P. salmonis after exposure to salmonid skin mucus. The results showed that while skin mucus did not significantly affect bacterial growth, it delayed the cytotoxic response of the bacteria towards cells.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Macarena Echeverria-Bugueno, Cristobal Balada, Rute Irgang, Ruben Avendano-Herrera
Summary: The study found that Renibacterium salmoninarum is able to produce extracellular vesicles (EVs), which play a crucial role in host invasion and exhibit toxic effects. This information lays the foundation for potential applications of R. salmoninarum EVs in salmon aquaculture, inspiring further research and development against bacterial kidney disease.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Sara Valdes, Rute Irgang, Maria C. Barros, Pedro Ilardi, Monica Saldarriaga-Cordoba, Javier Rivera-Bohle, Enrique Madrid, Johana Gajardo-Cordova, Ruben Avendano-Herrera
Summary: The study reported the first isolation of Tenacibaculum maritimum in rainbow trout farmed in Chile, confirming its pathogenic potential in Atlantic salmon and expanding the known host distribution. The research highlighted the need for further studies to evaluate the risk of T. maritimum for the O. mykiss farming industry.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Ruben Avendano-Herrera, Pablo Saldivia, Jorn Bethke, Cristian Vargas, Mauricio Hernandez
Summary: The study revealed that under iron-limited conditions, the H-2 strain of R. salmoninarum demonstrated better adaptation and had a significantly enriched iron ion homeostasis pathway and key virulence factors. Iron deficiency favored the expression of the 57-kDa protein in strain H-2, the principal virulence factor for R. salmoninarum.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Maria F. Morales-Rivera, Diego Valenzuela-Miranda, Valentina Valenzuela-Munoz, Gustavo Nunez-Acuna, Ruben Avendano-Herrera, Cristian Gallardo-Escarate
Summary: This study aimed to characterize the bacterial community associated with Caligus rogercresseyi in Chilean salmon aquaculture. It found that sea lice can act as potential reservoirs for fish diseases in the aquatic environment.
Review
Fisheries
Ruben Avendano-Herrera, Marcos Mancilla, Claudio D. Miranda
Summary: Chile, the second largest producer of salmon, is facing concerns over the intensive use of antimicrobials in its salmon farming industry. This review provides a comprehensive analysis of the historical and current use of antimicrobials in Chilean salmonid farming, as well as the impacts on the environment and antibiotic resistance. It aims to clarify outdated or poorly supported information in existing literature and offers recommendations for reducing antimicrobial use and controlling bacterial fish pathogens.
REVIEWS IN AQUACULTURE
(2022)
Editorial Material
Immunology
Hector A. Levipan, Luis Reyes-Garcia, Ruben Avendano-Herrera
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Ruben Avendano-Herrera, Anne Berit Olsen, Monica Saldarriaga-Cordoba, Duncan J. Colquhoun, Victor Reyes, Javier Rivera-Bohle, Eric Duchaud, Rute Irgang
Summary: This study confirms the presence of Tenacibaculum piscium in Chile and Coho salmon, expanding the geographical and species distribution of this bacterium. The pathogenicity of the Chilean isolates was found to be low in Atlantic salmon, with observed scale loss and fin damage in dead fish. The whole-genome analysis revealed various genes associated with iron acquisition, secretion systems, antibiotic resistance, and stress responses.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Fisheries
Carla Fernandez-Espinel, Marco Medina-Morillo, Rute Irgang, Giovanna Sotil, Henry Araya-Leon, Violeta Flores-Dominick, Jesus L. Romalde, Ruben Avendano-Herrera, Jefferson Yunis-Aguinaga
Summary: This study aimed to identify and classify Yersinia ruckeri isolates from the Puno Region in Peru. Two biotypes were found among the isolates, but they shared identical fingerprints and serotypes. This information is important for the epidemiological research and vaccine development against Y. ruckeri.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Monica Saldarriaga-Cordoba, Henry Araya-Leon, Rute Irgang, Ruben Avendano-Herrera
Summary: This study developed a novel PCR assay for the specific detection of Tenacibaculum piscium, an emerging disease that severely affects salmonid farming in Chile. The PCR method showed high sensitivity for T. piscium in seeded fish samples, but lower sensitivity for DNA extracted from mucus samples. Compared to other existing methodologies, the PCR approach allowed for the easiest detection of T. piscium in mucus samples obtained from challenged fish.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Claudio D. Miranda, Rute Irgang, Christopher Concha, Rodrigo Rojas, Ruben Avendano-Herrera
Summary: This study identified the species Epilithonimonas ginsengisoli associated with fish farming for the first time. The isolate carried genes for antibiotic resistance but showed no pathogenic effects in challenge trials.
JOURNAL OF FISH DISEASES
(2023)
