Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 176, Issue -, Pages 244-252Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.02.006
Keywords
Polyhydroxyalkanaotes; PHBV; Fibroblasts; Biocompatibility; Hemocompatibility
Funding
- Centre for Marine Living Resources and Ecology, Ministry of Earth Sciences, Government of India [MoES/CMLRE 10-MLR-TD/09/2012]
- Department of Science and Technology (DST)
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PHBV films produced from Bacillus cereus MCCB 281 exhibit moderate surface roughness and hydrophilicity, showing good cell attachment and proliferation of L929 fibroblasts, as well as excellent blood compatibility for potential use in tissue engineering and medical applications.
Polyhydroxyalkanoates (PHAs) are natural polyesters produced by microorganisms as a source of intracellular energy reserves. These polymers have been extensively studied for tissue engineering and drug delivery applications due to their desirable material properties. Solvent-cast film of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), produced by Bacillus cereus MCCB 281 was characterized to study the surface morphology, roughness, thermal and mechanical properties. PHBV films were slightly hydrophilic with an average surface roughness of 43.66 nm. In vitro cell viability and proliferation studies on PHBV film surface investigated using L929 fibroblasts showed good cell attachment and proliferation. Hemocompatibility of PHBV evaluated by hemolysis assay, in vitro platelet adhesion and coagulation assays demonstrated good blood compatibility for use as blood contact graft materials. Therefore, PHBV produced from the marine bacterium favoured cellular growth of L929 fibroblasts indicating its potential to be used as a biomaterial substrate for cell adhesion in tissue engineering and medical applications. (c) 2021 Elsevier B.V. All rights reserved.
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