Journal
HUMAN MOLECULAR GENETICS
Volume 30, Issue 11, Pages 1006-1019Publisher
OXFORD UNIV PRESS
DOI: 10.1093/hmg/ddab100
Keywords
-
Funding
- Japan Agency for Medical AQ7 Research and Development (AMED) [19bm0804005-h0103, 20lm0203086h0002]
- Neurological and Psychiatric Disorders of the National Center of Neurology and Psychiatry intramural research grants [28-6, 30-9]
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [19K075190001]
Ask authors/readers for more resources
The study demonstrates that using SERCA activator CDN1163 can improve symptoms in dystrophin-deficient mice, prevent muscular damage, and enhance muscular strength. Continuous treatment for 7 weeks can reduce muscular degeneration and fibrosis. This research provides preliminary evidence for treating DMD by pharmacologically activating SERCA, and CDN1163 surprisingly improves muscular strength in wild-type mice.
Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder characterized by progressive muscular weakness because of the loss of dystrophin. Extracellular Ca2+ flows into the cytoplasm through membrane tears in dystrophin-deficient myofibers, which leads to muscle contracture and necrosis. Sarco/endoplasmic reticulum Ca2+ -ATPase (SERCA) takes up cytosolic Ca2+ into the sarcoplasmic reticulum, but its activity is decreased in dystrophic muscle. Here, we show that an allosteric SERCA activator, CDN1163, ameliorates dystrophic phenotypes in dystrophin-deficient mdx mice. The administration of CDN1163 prevented exercise-induced muscular damage and restored mitochondrial function. In addition, treatment with CDN1163 for 7 weeks enhanced muscular strength and reduced muscular degeneration and fibrosis in mdx mice. Our findings provide preclinical proof-of-concept evidence that pharmacological activation of SERCA could be a promising therapeutic strategy for DMD. Moreover, CDN1163 improved muscular strength surprisingly in wild-type mice, which may pave the new way for the treatment of muscular dysfunction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available